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961.
城市地理信息三维可视化研究 总被引:13,自引:1,他引:12
讨论的城市地理信息系统三维可视化技术致力于将传统的地理信息系统和虚拟现实技术结合起来,通过对图形数据和属性数据库的共同管理、分析及操作,实现数据可视化,为城市管理和决策提供准确、及时、全面的信息、依据和手段,促进城市管理的规范和科学化。本文建立的三维可视化城市地理信息系统使用了细节层次(Level of Detail,LOD)显示技术和视景分块调度技术来提高实时性。 相似文献
962.
利用矩阵这一工具,研究了到3NF保持函数依赖的模式分解,文中基于矩阵给出了判定3NF的若干定理,并提出矩阵行相容,矩阵包含和矩阵合并等新的定义,给出了基于矩阵到3NF保持函数依赖的模式分解算法。 相似文献
963.
对—氯片呐酮与1,2,4—三唑缩合为1—(1,2,4—三唑基—1) —片呐酮时的分子比、溶剂、缚酸剂、反应时间等条件进行了研究,得到一种适合放大应用于工业生产的以乙醇为溶剂的最佳缩合条件。 相似文献
964.
运用Mip-map技术分析了纹理走样产生的主要原因,着重探讨了Mip-map技术在屏幕象素绘制过程中对纹理走样的抑制潜力,并针对"福大校园三维实时景观漫游"的实现,给出了应用结果. 相似文献
965.
DENGZhaoxiang QIUWenfeng LIWeijia LIYadong 《科学通报(英文版)》2004,49(2):127-130
Nitriles were unexpectedly trimerized into s-triazines or amino-pyrimidines in high yields in the presence of catalytic amount of Li3N, resulting in a simple, solvent-free and easy-to-scale-up one-pot way to synthesize s-triazines and 4-amino-pyrimidines with high yield. 相似文献
966.
967.
Isolation of human epidermal stem cells by adherence and the reconstruction of skin equivalents 总被引:47,自引:0,他引:47
Kim DS Cho HJ Choi HR Kwon SB Park KC 《Cellular and molecular life sciences : CMLS》2004,61(21):2774-2781
The isolation of human epidermal stem cells is critical for their clinical applications. In the present study, we isolated three populations of epidermal keratinocytes according to their ability to adhere to collagen type IV: i.e., rapidly adhering (RA), slowly adhering (SA), and non-adhering (NA) cells. The aim of this study was to characterize RA cells and to investigate the possibility of using these cells for epidermis reconstruction. To identify RA cells, flow cytometric analysis was performed using anti-6 integrin and anti-CD71 antibodies. RA cells express high levels of 6 integrin and low levels of CD71, which are considered as markers of an epidermal stem cell nature. Furthermore, electron microscopy showed that RA cells are small and have a high nuclear to cytoplasmic ratio, whereas SA and NA cells have well-developed cellular organelles and abundant tonofilaments. Western blot analysis showed that RA cells are slow cycling and express p63, a putative epidermal stem cell marker, whereas SA and NA cells express c-Myc, which is known to regulate stem cell fate. To compare epidermal regenerative abilities, skin equivalents (SEs) were made using RA, SA, and NA cells. The epidermis constructed from RA cells was well formed compared to those formed from SA or NA cells. In addition, only SEs with RA cells expressed 6 integrin and 1 integrin at the basal layer. These results indicate that RA cells represent epidermal stem cells and are predominately comprised of stem cells. Therefore, the isolation of RA cells using a simple technique offers a potential route to their clinical application, because they are easily isolated and provide a high yield of epidermal stem cells.Received 2 July 2004; received after revision 20 August 2004; accepted 10 September 2004 相似文献
968.
969.
Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines 总被引:3,自引:0,他引:3
Wang P Mariman E Keijer J Bouwman F Noben JP Robben J Renes J 《Cellular and molecular life sciences : CMLS》2004,61(18):2405-2417
Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of
adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to
profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation
was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment
were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as
secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25
are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an
actively remodeling extracellular matrix and several factors involved in growth regulation.Received 15 June 2004; received after revision 26 July 2004; accepted 2 August 2004 相似文献
970.
Apoptotic cell death in the lactating mammary gland is enhanced by a folding variant of α-lactalbumin 总被引:1,自引:0,他引:1
Apoptosis is essential to eliminate secretory epithelial cells during the involution of the mammary gland. The environmental regulation of this process is however, poorly understood. This study tested the effect of HAMLET (human -lactalbumin made lethal to tumor cells) on mammary cells. Plastic pellets containing HAMLET were implanted into the fourth inguinal mammary gland of lactating mice for 3 days. Exposure of mammary tissue to HAMLET resulted in morphological changes typical for apoptosis and in a stimulation of caspase-3 activity in alveolar epithelial cells near the HAMLET pellets but not more distant to the pellet or in contralateral glands. The effect was specific for HAMLET and no effects were observed when mammary glands were exposed to native a-lactalbumin or fatty acid alone. HAMLET also induced cell death in vitro in a mouse mammary epithelial cell line. The results suggest that HAMLET can mediate apoptotic cell death in mammary gland tissue.Received 30 January 2004; received after revision 5 March 2004; accepted 16 March 2004 相似文献