Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)

Severe Acute Respiratory Syndrome (SARS) is a newly identified infectious disease[1—5]. The global outbreak of SARS has been threatening the health of people worldwide and has killed 353 people and infected more than 5462 in 27 countries, as reported by WHO on April 29, 2003 (http://www.who.int/csr/sarscountry/en). Although it has been recognized that a variant of virus from the family of coronavirus might be the candidate pathogen of SARS[1—5], its identity as the unique pathogen sti…     

新型Cu(II)双核配合物的合成、结构表征和量化计算

以二(2-苯并咪唑亚甲基)胺(N3)为配体,合成了一种新型Cu(II)双核配合物作为超氧化物歧化酶(SOD)的模型化合物.采用元素分析、UV和IR光谱进行了表征,应用X射线衍射方法测定了晶体结构,并利用G98W程序在HF/LANL2DZ基组水平上对该双核配合物进行了量子化学计算.晶体结构分析表明,Cu(II)双核配合物的化学式量分子(C42H40N12O17Cl4Cu2)是以4,4′-联吡啶为桥的Cu(II)双核配合物单元,Cu(II)与配位原子形成变形八面体构型,轴向配位氧原子来源于高氯酸根.量化计算所得原子轨道贡献和原子净电荷布居分析结果与晶体结构中的配位情况相符.     

铂(II)与胺类配合物的合成、晶体结构和抗肿瘤活性研究

研究铂(II)与三种胺类配合物的合成、晶体结构和体外抗肿瘤活性,化合物1的X-射线单晶结构分析表明:这是一个很少见的具有四方锥几何构型的铂(II)配合物,顺式平面四方形配位的铂的轴向上有第5个配位原子。其抗肿瘤活性实验显示3个配合物对几种研究的癌细胞都有较强的抑制作用。     

呼气未正压通气治疗急性肺水肿

目的:评价呼气末正压通气(PEEP)治疗急性肺水肿的临床疗效。方法:30例急性肺水肿患者,在药物治疗基础上,加用PEEP(3～8cmH_2O)机械通气治疗,对比治疗前后血压(BP)、呼吸(R)、心率(HR)、氧饱和度(SaO_2)等动脉血气指标及胸片变化,同时与30例常规药物治疗组进行对比。结果:经PEEP机械通气治疗0.5～1h后,29例呼吸频率减慢、心率减慢,氧饱和度上升,血气指标好转,心功能改善,仅一例无效死亡。总有效率96.67％。结论:呼气末正压通气是治疗急性肺水肿快速、有效的方法。     

南极伊利莎白公主地250年来NO-3浓度变化特征研究

利用1996～1997年中国首次南极内陆冰盖考察获得的50m雪芯资料,详细研究了南极伊利莎白公主地250年来NO -3 浓度变化特征.结果表明:在250年尺度上,本地的NO -3 浓度具有明显的季节变化特征.250年来,伊利莎白公主地NO -3 浓度与太阳活动没有相关关系,认为本地的NO -3 可能主要来源于中低纬度地区的闪电和极地高空中的各种大气过程,雪冰中记录的NO -3 浓度可能是其来源、传输路径和沉积过程的综合反映.     

pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes

IntroductionPhotosystem II ( PSII) is a pigment- proteincomplex in the thylakoid membrane. Its reactioncenter( PSII- RC) ,which is composed of D1 andD2 proteins,generates the highly positive oxidantrequired for the oxidation of water by light- drivencharge separation. Water oxidation occurs at theMn4cluster positioned atthe center of the oxygen-evolving complex on the lumenal surface of PSII.In green plants,the highly reactive Mn4cluster isshielded by a number of extrinsic proteins( 33…     

Shakedown Analysis of 3-D Structures Using the Boundary Element Method Based on the Static Theorem

The static shakedown theorem was reformulated for the boundary element method (BEM) rather than the finite element method with Melan‘s theorem, then used to develop a numerical solution procedure for shakedown analysis. The self-equilibrium stress field was constructed by a linear combination of several basis self-equilibrium stress fields with undetermined parameters. These basis self-equilibrium stress fields were expressed as elastic responses of the body to imposed permanent strains obtained using a 3-D BEM elastic-plastic incremental analysis. The lower bound for the shakedown load was obtained from a series of nonlinear mathematical programming problems solved using the Complex method. Numerical examples verified the precision of the present method.     

光滑流形在(Z2)2作用下信息为{(2,2,0),(2,0,2),(0,2,2)}的上协边类

设(Z2)^2作用于光滑闭流形M^n，其不动点集的法丛的信息为P＝{(2，2，0)，(2，0，2)，(0，2，2)}，Jn^4，2(P)是有代表元M^n且具有上述性质的n维上协边类[M^n]构成的集合．作者通过构造上协边环M0n的一组生成元决定了Jn^4，2(P)的群结构．     

2-氨基-3-氰基-4-(2’,4’-二氯苯基)-4H-萘并[1,2-b]吡喃的合成和晶体结构

标题化合物C22H17Cl2NO3的晶体由α-乙氧羰基-2,4-二氯肉桂腈、β-萘酚在TEBA存在下合成并经DMF与水的混合溶剂重结晶而得.其结构通过元素分析、IR和1HNMR进行了表征,用X射线单晶衍射法测定了该化合物的晶体结构.结果表明,晶体属于单斜晶系,P21/n空间群。a=1.450 5(2)nm,b=0.903 7(1)nm,c=1.491 3(2)nm,α=90°,β=101.21(1)°,γ=90°,Mr=414.27,V=1.917 5(4)nm3,Dc=1.435 g·cm-3,Z=4,μ(MoKα)=0.362 mm-1,F(000)=856.晶体结构用直接法解出,经全矩阵最小二乘法修正,最终偏离因子R=0.044 44,Rw=0.117 7.化合物分子中,原子C(1),C(2),C(3),C(4),C(5)和O(1)形成一个六元环,该六元环中C(1)-C(2)键长为0.134 6(3)nm,表明为双键.此六元环为船式构象.