Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus

Functional analysis for gene silencing suppressor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- infiltration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP)expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by homologous sequence was strongly suppressed by the immixture infiltration of either the P14 or the $6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results suggested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors.     

RNAi-mediated knockingdown of rlpk2 gene retarded soybean leaf senescence

Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll.     

Functional identification of gene cluster for the aniline metabolic pathway mediated by transposable element

A convenient and widely applicable method has been developed to clone aniline metabolic gene cluster in this study. Three positive recombinant plasmids pDA1, pDB2 and pDB11 were cloned from genomic library of aniline degradation strain AD9. The result of aniline dioxygenase （AD） activity and catechol 2,3-oxygenase （C230） activity assay showed that pDA1 and pDB11 contain aniline dioxygenase genes and catechol 2,3-dioxygenase genes, respectively. The sequence analysis of the total 24.7-kb region revealed that this region contains 25 ORFs, of which 17 genes involve metabolism of aniline. In the gene cluster, the first five genes （tadQTA1A2B） and the subsequent gene （tadR1） were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others （tadD1C1D2C2EFGIJKL） were expected to encode metacleavage pathway enzymes for catechol degradation. The gene cluster was surrounded by two IS1071 sequences.     

太原市污染物浓度时空分布特征及影响因子分析

对2003年太原市污染物浓度逐日数据和气象资料进行了分析，阐述了污染物浓度的时空变化和污染物浓度与气象条件的关系，为减轻和防止大气污染提供了参考。     

用计算机对人类TSPYl基因P53结合位点的鉴定

根据p53下游基因在其调节区域（启动子或内含子）含有与P53蛋白特异性结合的一致性序列5’-RRRCWWGYYYN（0-13）RRRCWWGYYY-3’，R—G或A，W—T或A，Y—C或T，N—A，C，T，G。用计算机对人类基因组中P53结合位点进行了研究，发现Y染色体上的TSPY1基因内含子中含有这样的一致性序列5’-GGGCTAGTTTtgGAGCTAGCCT-3’，意味着TSPY1基因有可能是一个p53下游基因。     

科学发展观与"十一五"规划的制定 ——学习党的十六届五中全会精神的体会

本文论述了科学发展观的基本内涵,阐明了科学发展观是马克思主义发展理论与当代中国实际和时代特征相结合的最新成果。“十一五”规划是科学发展观的行动纲领。推进社会主义和谐社会建设是全面贯彻落实科学发展观的体现,也是用科学发展观统领经济社会发展全局的体现。     

(C0)类等度连续半群的无穷小生成元的特征

主要讨论在局部凸线性拓扑空间上的(C0)类等度连续半群{T(t):t≥0}诱导的C0-半群拓扑意义下,{T(t):t≥0}的一些基本性质,以及(C0)类等度连续半群{T(t):t≥0}在C0-半群拓扑意义下以及原拓扑意义下的无穷小生成元之间的关系.     

一致凸Banach空间的一个新的特征性质

给出了Banach空间一致凸的一个新的充要条件:设λ,μ∈(0,1),λ μ=1,f:R R 是单调递增且可微的严格凸函数,X是Banach空间,则X是一致凸的当且仅当对任意ε>0,存在δ>0,使得当‖x‖≤1,‖x-y‖≥ε时,有f(‖λx μy‖)<λf(‖x‖) μf(‖y‖)-δ     

西藏冬虫夏草无性型5.8S rDNA,ITS间区系统发育分析

 采用相对较低的培养温度(5~12℃),从西藏那曲当年产风干冬虫夏草[Cordyceps sinensis(Berk.)Sacc.]分离得到菌株C21,综合其菌丝形态学特征及5.8S rDNA和ITS间区的系统发育分析结果,证实菌株C21为冬虫夏草菌的无性型,即中国被毛孢(Hirsutella sinensis).     

超凸度量空间中的变分不等式和极大极小不等式

文章给出了超凸度量空间中的一些变分不等式定理和极大极小不等式定理。