加强辅酶循环再生实现不对称合成(S)-1-苯基-1,2-乙二醇

以β-羟基苯乙酮为底物,利用羰基还原酶不对称合成(S)-1-苯基-1,2-乙二醇(PED)过程中,葡萄糖脱氢酶和6-磷酸葡萄糖脱氢酶催化NADPH再生反应与还原反应形成耦联,合成(S)PED的浓度分别达到0.78 g·L-1和0.75 g·L-1,对映体过量值(e.e.%)分别为91.84％和63.96％;连续分批还原反应后其总转化数(TTN)为26和58;经硫酸铵盐析、离子交换层析DEAE Sepharose、苯基疏水层析Phenyl Sepharose和亲和层析Blue Sepharose后得到电泳纯     

Ultrafast polarization optical switch constructed from one-dimensional photonic crystal and its performance analysis

All-optical switch with the ultrafast optical switching rate is a key device in the next generation optical network. In this article, we propose a polarization switch with ps switching time, which is constructed from one-dimensional resonant photonic crystal （1D RPC）. The model of switch operating at 1.5 μm is established based on the optical stark effect （OSE）. We calculate the performance indices of the switch and the influences of errors of periods and refractive index on the performance characteristics.     

Windows 2000环境下的一种SCSI冗余路径驱动程序

为提高存储系统的可用性 ,在 Windows2 0 0 0系统中实现了SCSI冗余路径驱动程序 RDP。在路径发生故障时由 RDP将 I/ O切换到其他路径。通过将 I/ O负载均衡分布在各 SCSI路径 ,能增加系统 I/ O处理能力     

Emodin inhibits tumor cell migration through suppression of the phosphatidylinositol 3-kinase-Cdc42/Rac1 pathway

Enhanced cell migration is one of the underlying mechanisms in cancer invasion and metastasis. Therefore, inhibition of cell migration is considered to be an effective strategy for prevention of cancer metastasis. We found that emodin (3-methyl-1,6,8-trihydroxyanthraquinone), an active component from the rhizome of Rheum palmatum, significantly inhibited epidermal growth factor (EGF)- induced migration in various human cancer cell lines. In the search for the underlying molecular mechanisms, we demonstrated that phosphatidylinositol 3-kinase (PI3K) serves as the molecular target for emodin. In addition, emodin markedly suppressed EGF-induced activation of Cdc42 and Rac1 and the corresponding cytoskeleton changes. Moreover, emodin, but not LY294002, was able to block cell migration in cells transfected with constitutively active (CA)-Cdc42 and CA-Rac1 by interference with the formation of Cdc42/Rac1 and the p21-activated kinase complex. Taken together, data from this study suggest that emodin inhibits human cancer cell migration by suppressing the PI3K-Cdc42/Rac1 signaling pathway.Received 7 February 2005; received after revision 11 March 2005; accepted 18 March 2005     

A novel pattern of fast calcium oscillations points to calcium and electrical activity cross-talk in rat chromaffin cells

Slow oscillations of cytosolic calcium ion concentration – – typically originate from release by intracellular stores, but in some cell types can be triggered and sustained by Ca²⁺ influx as well. In this study we simultaneously monitored changes in and in the electrical activity of the cell membrane by combining indo-1 and patch-clamp measurements in single rat chromaffin cells. By this approach we observed a novel type of spontaneous oscillations, much faster than those previously described in these cells. These oscillations are triggered and sustained by complex electrical activity (slow action potentials and spike bursts), require Ca²⁺ influx and do not involve release from intracellular stores. The possible physiological implications of this new pathway of intracellular signalling are discussed.Received 30 July 2004; received after revision 14 October 2004; accepted 1 November 2004     

Compensatory caspase activation in MPP+-induced cell death in dopaminergic neurons

Many have hypothesized that cell death in Parkinsons disease is via apoptosis and, specifically, by the mitochondrial-mediated apoptotic pathway. We tested this hypothesis using a mouse dopaminergic cell line of mesencephalic origin, MN9D, challenged with the Parkinsonism-causing neurotoxin MPP⁺ (1-methyl-4-phenylpyridinium ion). Apoptosis was the main mode of cell death when the cells were subjected to MPP⁺ treatment under serum-free conditions for 24 h. Caspase-3 and caspase-9, however, were not activated, thus indicating the existence of alternate or compensatory cell death pathway(s) in dopaminergic neuronal cells. Using caspase inhibitors, we demonstrated that these pathways involve caspase-2, –8, –6 and –7. A time-course study indicated that activation of caspase-2 and –8 occurred upstream of caspase-6 and caspase-7. Upon MPP⁺ challenge, the apoptosis-inducing factor was translocated from the mitochondria into the MN9D cytosol and nucleus. These results suggest the existence of alternative apoptotic pathways in dopaminergic neurons.Received 20 September 2004; received after revision 5 November 2004; accepted 22 November 2004     

分子电子学

作为纳米电子学的一个重要分支,分子电子学在近年来得到了巨大的发展,并成为国际上研究的热点。本文介绍了各种分子器件的制作技术及基本工作原理,回顾了近年来分子电子学的最新进展,展望了分子电子学的未来发展。     

基于改进自抗扰控制的微电网混合储能控制策略

储能系统在微电网保证电力系统运行稳定运行中起到了关键的作用.因可再生能源发电具有许多不确定因素,从而引发的随机性以及波动性对微电网并网运行产生恶劣的影响.针对以上问题,为提高微电网并网的电能质量,减小直流(direct current,DC)母线电压的波动和冲击,提出一种由超级电容与蓄电池组成的改进自抗扰控制(acti...     

浅析1-甲基环丙烯对秋番茄果实保鲜的应用

1-MCP（1-甲基环丙烯）是一种新型乙烯作用抑制剂,它能不可逆地作用于乙烯受体,阻断与乙烯的正常结合,能有效地抑制植物对内源或外源乙烯的敏感性,在园艺商业上具有很大的潜在应用价值.本文综述1-MCP在水果、花卉、蔬菜贮运保鲜应用研究方面的进展及应注意的问题。