ICP-AES比较临床分离药物敏感和耐药结核分枝杆菌的金属离子

利用现在多元素痕量分析最重要的方法——电感耦合等离子体原子发射光谱(ICP—AES)分析结核分枝杆菌中的金属离子的种类和含量,结果表明,Cr^3 ,Al^3 ,Ca^2 ,Fe^2 ,Mn^2 ,Co^2 ,Cu^2 ,Ni^2 的含量较高,并发现药物敏感菌株和耐药菌株之间在金属离子种类和含量上有明显区别．这种差异在耐药中的意义有待进一步研究．     

Degradation of pyrene and characterization of<Emphasis Type="Italic">Saccharothrix</Emphasis> sp. PYX-6 from the oligotrophic Tianchi Lake in Xinjiang Uygur Autonomous Region,China

Bacterial strain PYX-6 that utilizes anthracene,phenanthrene, or pyrene for carbon and energy sources for growth was isolated from a non-polluted lake (Tianchi Lake) in Xinjiang Uygur Autonomous Region of China. Its morphology, physiological and biochemical properties, cell wall pattern and G C mol% content of DNA molecules were characterized. The 16S rRNA gene of strain PYX-6 was sequenced and analyzed for similarities to related bacterial species. Results indicated that strain PYX-6 is a member of the Genus Saccharothrix, and the strain was named Saccha-rothrix sp. PYX-6. When pyrene was the sole carbon source in cultural medium, the strain PYX-6 assimilated pyrene for growth and 0.005% of yeast extract stimulated pyrene degradation and assimilation. The optimal pH of cultural medium and the optimal shaking frequency during cultivation were 6-8 and 200 r/m, respectively. It was found that the disappearance of pyrene in medium occurred before significant growth of strain PYX-6 took place. Phthalic acid, benzylacetic acid, and benzylpropenoic acid were detected as catabolic intermediates during pyrene degradation with mass spectroscopy and this result indicated that Saccharothrix sp.PYX-6 adopted a pathway that is different from the pathway of the previously reported pyrene-degrading Mycobacterium sp. PYR-1.     

嗜热硫氧化硫化杆菌一新菌株的分离与鉴定

从云南腾冲酸性热泉富集物中分离到一株适度嗜热喜酸菌YN22.该菌革兰氏染色阳性,直杆状或微弯,长约1.6~2.8μm,直径0.4~0.7μm.该菌能在25~60℃下生长,最适生长温度为53℃;生长pH为1.0~5.0,最适pH为1.5;化能自养型,0.025%(w/V)的酵母提取物对其生长有明显的促进作用,在酵母提取物存在的情况下能快速氧化Fe2 ,但对S0和还原型硫化物的氧化能力较低.16s rDNA系统发育分析表明,该菌与嗜热硫氧化硫化杆菌(Sulfobacillus thermosulfidooxidans)的16s rDNA序列相似性达99%.YN22基因组DNA的G C含量为47.3 mol%,与嗜热硫氧化硫化杆菌模式菌株VKM B-1 269非常接近,后者基因组DNA的G C含量为47.5 mol%.基于形态特征、生理生化特性、系统发育学和G C含量的分析结果,YN22应归于硫化杆菌属(Sulfobacillus),为嗜热硫氧化硫化杆菌(Sb.thermosulfidooxidans)的一新菌株.这是国内首次分离,并经多种方法鉴定、确认的嗜热硫氧化硫化杆菌,从而为我国浸矿微生物的基础和应用研究提供了最典型的适度嗜热菌种.     

杂交油菜冷害原因及预防

油菜是河南省第三大油料作物.油菜作为秋冬播作物,由于收获较小麦、豌豆早,所以,对下茬夏播作物的早播、高产提供了有利条件.然而,近些年来,由于诸多原因,河南省油菜常受冷害侵袭,造成减产.     

M-海因水解酶系统菌株的筛选及发酵条件优化

以M-海因为唯一碳源、氮源筛选出7株具有M-海因水解酶系统活性的菌株,其中一个菌株M003的M-海因水解酶活性较高,能将M-海因转化为L-Met。对其发酵条件及培养基进行了优化,结果表明最适培养基为(g/L):M-海因5,蔗糖10,NH4Cl1,K2HPO43,KH2PO41,MgSO40.5,FeSO40.1,MnSO40.01,37℃,pH7.5,在优化条件下,L-甲硫氨酸产量达到1.1g/L。     

苏州市农业科技创新巡礼之三"常优1号"与端木银熙

早就听闻常熟有个“袁隆平”——常熟市农科所高级研究员端木银熙,这位农民出身的水稻专家从事水稻研究40多年,先后带领着他的团队承担了省、市“八五”、“九五”、“十五”农业科技重大攻关项目——优质高产多抗水稻新品（组合）的“常优1号”选育工作。“常优1号”是江苏第一个通过国家级审定的杂交晚粳稻品种，创下了实收亩产808．6公斤的高记录，     

无公害农药与生物防治技术

农产品质量安全,关系到广大人民群众的身体健康,关系到经济社会协调发展,关系到构建社会主义和谐社会,深受政府及社会各界高度关注。1992年,我国政府作出了发展高产优质高效农业的决定,农产品质量安全工作     

提高综采面设备及系统可靠性的主要途径

针对高产高效综采面配套设备的技术特征，适应开采煤层的特点及目前我国高产高效综采面配套设备存在的有关问题进行分析，探讨我国高产高效综采面设备及系统的生产能力和可靠性。