Recombinant PsbF from Synechococcus sp. PCC 7002 forms β:β homodimeric cytochrome b559

All organisms with oxygenic photosynthesis contain two photosystems:photosystem Ⅰ(PSⅠ)and photo-systemⅡ(PSⅡ),The minimal photosystem Ⅱ particles which are photochemically active contain three subunits:D1,D2 and cytochrome b559 (Cyt b559),The function of Cyt b559 remains unclear,We have successfully overxpressed the psbF gene,encoding the β subunit of Cyt b559,from a marine cyanobacterium Synechoccous sp.PCC 7002 as a fusion gene and obtained a redox-active from of Cyt b559,When the N-terminal GST protein of the fusion gene product was removed with thrombin ,the PsbF protein was still redox-active,suggesting that the recombinant PsbF can form dimer in Escherichia coli.The absorption spectra of either the oxidized from or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference Spectra between the two forms are the same as that of the Cyt b559 isolated from the higher plants .Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559 ,The results are helpful to the understanding of locatlization and function of Cyt b559 on thylakoid membranes.Ⅰ     

Assignment of Soret MLCT band of reduced form of copper binuclear cluster in cytochrome c oxidase film

Low concentration of dithionite results in the reduction of Cu-Cn binuclear and heine a active sites of the cytochrome c oxidase thin solid film immersed in the acidic phosphate buffer, but Fe-Cu binuclear center keeps in the oxidation state. It manifests as a negative peak at 426 nm and a positive one at -408 nln in the difference spectra induced by dithionite. The former implies decrease of the oxidized form of heme a center, that is, Fea^3 →Fea^2 . And the latter results from the contribution of metal-ligand charge transfer (MLCT) transition in the reduced binuclear Cu-Cu cluster, rather than from that of heine a center. This stronger Soret MLCT band must be helpful to overcoming the difficulty in distinguishing the weaker copper sign from the stronger one of iron when studying copper-iron protein.     

Regional patterns of particulate spectral absorption in the Pearl River estuary

Knowledge about the spectral absorption properties of marine particulate matters is of important signification for various goals of optical oceanography, among them the most concerns are the bio-optical algorithms for ocean color remote sensing[1], basic theory of light radiative transfer in water column[2], bio-optical models for estima-tion of primary productivity and simulation of the upper ocean ecological process[3,4]. The spectral absorption by marine particulate matter is combined influe…     

催化动力学光度法测定痕量锰

实验研究了在NaOH介质中锰催化过氧化氢桑色素褪色反应及其动力学条件,测定了反应级数和表观活化能,建立了测定痕量锰的新方法。该方法检出限为9.34×10-7g/L,线性范围为3.2×10-5~1.44×10-4g/L。可用于水中痕量锰的测定。     

和田玫瑰花红色素的提取及性质研究

文章介绍了和田玫瑰花红色素的提取,研究了光,温度,酸碱性,氧化剂,还原剂,防腐剂,添加剂,金属离子对和田玫瑰花红色素稳定性的影响与理化性质进行了初步研究。玫瑰花红色素的耐光性较好,耐热性很好,可做食品的着色剂,但在使用过程中应尽量避免长时间光照。添加剂对红色素的影响不大,防腐剂对红色素影响不大。Mg2 ,Ba2 ,Na ,K ,Cd2 ,Ca2 对色素的稳定性无影响,Al3 ,Cu2 ,Fe3 对色素有一定影响,在提取和保存过程中应避免使用铁器。     

楔叶茶蔗（Ribes diacanthum Pall．）果实营养成分及其食用价值评价

对楔叶茶藤果实的营养成分进行测定。结果表明，其阴干果实富含糖、粗蛋白、粗脂肪、粗纤维、维生素C、维生素B1、维生素B2、维生素PP、维生素A、维生素E、胡萝卜素、10种矿质元素和18种氨基酸等丰富的营养物质,具有很高的食用价值，可以将其开发为内蒙古兼有经济和生态双重功能的乡土资源植物．     

Linear and third-order nonlinear optical properties for the heptamethine cyanine chromophore H-aggregates thin film

The thin film of a heptamethine cyanine chromophore HC was prepared by spin-coating technique. Its surface morphology and linear optical property were characterized by atomic force microscopy (AFM) and UV-visible absorption spectroscopy. The results show that HC molecules are arranged in a well-ordered H-aggregate type. The third-order nonlinear optical properties of the spin-coating film were also measured by degenerate four-wave mixing (DFWM) measurement. Enhanced third-order nonlinear susceptibility can be attributed to molecular aggregation effects, and the corresponding mechanism was dealt with by collective electronic oscillator (CEO) approach.     

沙棘黄色素的提取及性质实验

对从沙棘果实中提取纯天然植物黄色素的工艺进行了探讨，并通过实验，测试了该色素的性质。     

刍议食用香精香料的现状及发展趋势

随着生活水平的提高,人们对食品安全的关注度越来越高.而食品的风味对于人们的选择以及消费也起着至关重要的作用,并且在食品的生产过程中,企业为了提高食品的香气以及特有的风味,往往会加入天然或人工合成的食用香精香料来达到目的.文章就食用香精香料的现状展开探讨,进一步分析其在工业文明以及人们的日常生活中的应用,并对其未来的发展趋势进行了合理的展望.     

用水萃取法提取仙人果中的玫瑰红色素

以水为萃取剂,加入L-抗坏血酸作抗氧化剂、果胶酶降解果胶、改性甲壳胺吸附蛋白质.提取仙人果中的玫瑰红色素,此法萃取率高、工艺简单、无污染.