抗烟毒素基因的分子克隆及其序列分析

烟草野火病是烟草生产上的主要病害之一,在我国云南等主要烟草栽培地区常年发生,一般发病率为40—50%,重病田发病率高达90%以上,危害十分严重;而且收获后在烘烤过程中病斑还会明显增大,损失惨重,为此探索有效的防治途径是烟草生产上的当务之急.野火病菌侵染烟草叶片后,产生一种细菌毒素即烟毒素(tabtoxin),干扰寄主细胞代谢,并间接影响叶绿素的合成,形成野火病症.该毒素是非专一性的,除烟草之外,对多种植物、     

一种病毒蛋白B细胞表位预测方法的建立

病毒蛋白抗原表位的预测对于设计免疫原性多肽、新型疫苗分子及诊断试剂均有帮助.但是,既往的预测方法并不多,且至少存在以下两方面的缺陷:(1)既往方法均基于一般蛋白质的实验结果,因此,到目前为止还没有1种专用于病毒蛋白的表位预测方法;(2)80年代后期以来,多肽固相自动合成技术和Geysen氏pin ELISA技术在免疫学的广泛应用、模拟位     

辣椒细菌性斑点病病原的鉴定及药剂防治试验

１９８８～１９９０年从石河子辣椒病叶上分离到１７个菌株，经形态学特征、染色反应、培养性状、生理生化特性、致病性等测定，确定这１７个菌株为油菜黄单胞菌疱斑致病变种即Ｘａｎｔｈｏｍｏｎａｓｃａｍｐｅｓｔｒｉｓｐｖ．ｖｅｓｉｃａｔｏｒｉａ（Ｄｏｉｄｇｅ）Ｄｏｗｓｏｎ。选用５种药剂分１０个浓度，经室内药效测定和田间药剂喷洒试验，其中以硫酸链霉素和ＣＴ效果最好。     

中华鳖水泡病病原病理学研究

１９９３年８月信阳师院生物系特种动物养殖研究所的试验鳖场孵化出一批小鳖。同年１０月有一只鳖得了一种新的疾病，病鳖颈根部和右下肢基部各有一大水泡，暂名“水泡病”。本文对其分离菌进行了研究，鉴定为嗜水气单胞菌。中华鳖水泡病的致病机理，可能与病原菌的毒力与传染途径有关，而鳖的抗病能力是能否引起该病的关键，外界环境因素是引起该病的诱因。     

毛竹基腐病病原的研究

毛竹基腐病是于１９７５年在江苏省常熟市首次发现的新病害，现已知浙江，安徽，江西、湖北和四川均有分布，国外至今未见报道，经多年的分离培养试验，从病株组织内获得４种真菌和１种细菌的纯培养，通过竹笋期的室内，室外和有伤，无伤的多次反复接种和再分离试验，结果表明：这种细菌对毛竹不有任何致病力，茁芽短梗霉和交链孢对毛竹只有很轻微的致病力，仅造成局部组织的坏死，暗孢节菱孢和异孢镰刀菌对毛竹有极强的致病力，并能     

Overinhibition of Mitogen-Activated Protein Kinase Inducing Tau Hyperphosphorylation

To reveal the relationship between mitogen-activated protein kinase (MAPK) and tau phosphorylation, we used different concentration of PD98059, an inhibitor of MEK (MAPK kinase), to treat mice neuroblastma (N2a) cell line for 6 h. It showed that the activity of MAPK decreased in a dose-dependent manner. But Western blot and immunofluorescence revealed that just when the cells were treated with 16μmol/L PD98059, tau was hyperphosphorylated at Ser396/404 and Ser199/202 sites. We obtained the conclusion that overinhibited MAPK induced tau hyperphosphorylation at Ser396/404 and Ser199/202 sites.     

Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus

Functional analysis for gene silencing suppressor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- infiltration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP)expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by homologous sequence was strongly suppressed by the immixture infiltration of either the P14 or the $6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results suggested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors.     

双向电泳联用质谱技术研究水稻明恢63对细菌性条斑病侵染的应答

水稻细菌性条斑病是由细条病菌Xanthomonas oryzae pv. oryzicola (Xooc)引起的一种细菌性病害,是我国南方稻区的主要病害.为了探明水稻对细条病的应答机制,我们应用双向电泳联用质谱技术,研究了水稻品种明恢63在水稻细条病病菌侵染后不同时期的全蛋白变化,并对差异蛋白进行了鉴定以及分析归类.共鉴定出17种表达上调的蛋白和10种表达下调的蛋白,这些蛋白参与了水稻对细条病侵染的信号识别及防卫应答,其中包括信号转导类蛋白、防卫相关蛋白、代谢相关蛋白和参与蛋白质合成的蛋白等.