知识型员工沉默行为问题探析

随着知识经济的发展,知识型员工沉默行为已成为提升现代组织绩效的绊脚石。针对知识型员工的特征,以群体结构理论为基础,从知识型员工个体因素、角色知觉、角色期待等角度对员工沉默的原因进行探讨,并提出改善员工沉默的对策。     

英语专业专科生精读课沉默现象的调查与分析

本文针对当前大学英语精读课堂存在的沉默现象,对萍乡高等专科学校英语专业一年级专科生进行了抽样调查。通过问卷调查、课堂观察和课后访谈的方式,从学生、教师以及课堂环境因素的角度分析了造成学生课堂沉默的原因,并试图提出一些打破这种课堂沉默的策略。     

2010年十大科普事件

由中国科协牵头组织,热心科普事业的两院院士和科普界人士共同评选的2010年中国十大科普事件日前公布,张悟本事件和转基因食品安全等十大事件入选。这十大事件分别为:胡锦涛等党和国家领导人来到中国科技馆,同少年儿童一起参加体验科学活动;电视片《钱学森》热播,科学家题材首次进入重大理论文献片行列;"低碳"成为年度流行语,节能减排深入人心;上海世博会展示"科技改变城市生活",公众尽享科普盛宴;探月工程嫦娥二号发射成功,传媒直播推动航天科普升级;张悟本等退去光环,"科学养生"成传媒热点;     

转基因技术及其在水稻育种中的研究进展

以载体介导、基因直接导入和种质系统3类基因转化体系分别介绍了常用的几种转基因技术—农杆菌转化法、基因枪法、PEG介导法、电击法、花粉管通道法和浸泡转化法的原理及其在水稻育种上的最新研究进展,比较了各种技术的特点。最后对转基因技术在水稻育种上的前景作了展望。     

人类有望2013年接受动物器官移植

<正>据英国媒体报道,人类器官短缺迫使科学家们不断探索如何使用人类基因培育转基因猪,并将这些长在猪身上的器官移植到人类身上,同时避免免疫系统排斥。现在科学家们宣称,人——猪器官移植有望在2013年实现。美国匹兹堡大学一个研究团队在《柳叶刀》杂志上发表论文预测":新一代转基因猪的培育,可能改善细胞和角膜异种移植的效果。我们认为,今后两到三年内就可以投入临床实验。"     

敲除和过表达miRNA446以研究其靶基因APP1的表达模式

MicroRNA是一类小的非编码RNA,参与植物生长发育和胁迫应答等多种生理过程。在本课题组前期研究中,通过基因芯片数据发现了一系列在胁迫应答中表达量有变化的microRNA。其中以miRNA446的变化尤为显著。为进一步探求miRNA446在胁迫应答中的变化与功能,本实验通过在水稻中分别敲除和过表达miR446,检测干旱和冷胁迫下,miR446及其靶基因APP1的表达模式。结果表明,过表达株系中,miR446的表达量与野生型的持续升高不同,是先降后升的,而APP1基因由于受到高水平miR446的剪切作用而一直降低。敲除株系中,miR446的表达量被完全抑制,在整个胁迫过程中均保持接近于零的水平,而APP1在胁迫中也仅有微弱的先降后升趋势。     

Review and prospect of transgenic rice research

Rice is one of the most important crops as the staple food for more than half of the world＇s population. Rice improvement has achieved remarkable success in the past half-century, with the yield doubled in most parts of the world and even tripled in certain regions, which has contributed greatly to food security globally. Rapid population growth and economic development pose a constantly increased food requirement. However, rice yield has been hovering in the past decade, which is mainly caused by the absence of novel breeding technologies, reduction of genetic diversity of rice cultivars, and serious yield loss due to increasingly severe occurrences of insects, diseases, and abiotic stresses. To address these challenges, Chinese scientists proposed a novel rice breeding goal of developing Green Super Rice to improve rice varieties and realize the sustainable development of agriculture, by focusing on the following 5 classes of traits： insect and disease resistance, drought-tolerance, nutrient-use effi- ciency, quality and yield potential. As a modern breeding approach, transgenic strategy will play an important role in realizing the goal of Green Super Rice. Presently, many transgenic studies of rice have been conducted, and most of target traits are consistent with the goal of Green Super Rice. In this paper, we firstly review technical advances of rice transformation, and then outline the main progress in transgenic rice research with respect to the most important traits： insect and disease-resistance, drought-tolerance, nutrient-use efficiency, quality, yield potential and herbicide-tolerance. The prospects of developing transgenic rice are also discussed.     

Overexpression of OsRAA1 promotes flowering and hypocotyls elongation in Arabidopsis

Previously, OsRAA1, an AtFPF1 homologue gene, was found to play an important role in modulating rice root development. In the current study, OsRAA1 was overexpressed in Arabidopsis, and the transgenic plants showed early flowering and elongated hypocotyl phenotypes as compared with the wild-type under white-light conditions. The hypocotyls of transgenic lines were twice as long as those of wild-type plants under red-light conditions but were indistinguishable from those of the wild-type under blue and far-red light and darkness. In addition, the phenotypes of AtFPF1 transgenic lines were similar to those of OsRAA1 transgenic lines. These results suggested that OsRAAI/AtFPF1 protein is involved in regulating flowering time and plays an important role in the inhibition of hypocotyl elongation under continuous red light. The functions were preserved during the evolution.     

中国文化中“沉默”之意义的内涵与外延

沉默是人丰富情感的一种重要的表达方式。讨论了“沉默”在文化、艺术、法律、教育、书法、音乐,乃至建筑、数学方面的体现,简述了沉默在这些领域内表现出的内涵与外延。     

转betA/als基因棉花生存竞争力和基因漂流的调查

对导入乙酰乳酸合成酶突变基因als及来自大肠杆菌的胆碱脱氢酶基因betA的棉花纯合株系T3、T4代植株的田间农艺学性状和基因漂流进行了研究。 结果表明,与野生型对照鲁棉研19相比,转betA/als基因棉花涉及生存竞争力的一些农艺学性状,如种子繁殖能力、贮存后活力以及盐碱地条件下的经济产量等显著提高,转基因棉花的棉花纤维马克隆值下降,其它农艺学性状无明显差别;获得的转基因性状之一除草剂抗性遗传稳定,并在田间表达良好;在转基因棉花释放区面积为6?m×6?m的条件下,als基因通过花粉介导进入野生型棉花的频率随着非转基因棉花种植区与释放区之间的距离增大而迅速降低,采取适当的隔离距离（大于200?m）,可以避免外源基因逃逸事件的发生。但设置隔离区时应考虑昆虫对花粉传播等不确定因素的影响,适当加大隔离距离。