振动切削过程中参数选择的研究

系统地研究了低频轴向振动切削过程,指出由于所加振动的影响,在切削过程中刀刃运动轨迹及切削参数都随时间发生变化。通过理论分析,建立了刀刃运动参数方程,并对切屑形成过程进行了充分的讨论,在此基础上,给出了振动切削时参数选择的原则。     

棒状杆菌亮氨酸操縱子在大肠杆菌中的克隆和表达

以质粒pTZ22为载体,用HindШ限制酶切割谷氨酸棒状杆菌染色体DNA进行克隆。分离到带有亮氨酸操纵子的4.5kb片段。用Southern切口移位分子杂交检测,证明在4.5kb的片段中既包含有leuB基因,而且对leuA基因也表现同源性。     

模型NEPE推进剂燃烧转爆轰实验研究

建立了推进剂燃烧转爆轰（ＤＤＴ）的实验系统，分别利用弱约束和强约束对ＮＥＰＥ推进剂的ＤＤＴ过程进行测试，得到了其速度增长规律和诱导爆轰距离，并对强弱约束的ＤＤＴ数据进行了比较．实验表明，在弱约束条件下由于侧向稀疏作用，推进剂较难产生由燃烧到爆轰的转变，一旦形成爆轰，稳定爆速将不受约束强度的影响．     

通用变频器差频控制系统设计

提出了利用通用变频器组成转差频率控制变频调速系统的设计方法，并提供了系统的构成与实验结果     

液晶高分子流体拉伸粘度的研究

利用液晶高分子共转Oldroyd流体B模型,研究了拉伸粘度的变化规律.作出了拉伸粘度随其它参数变化曲线.结论与实验结果一致.     

转抗除草剂基因油菜与5种十字花科杂草的基因流研究

通过荧光显微镜和人工杂交授粉结实情况的观察，研究了抗草甘膦甘蓝型油菜Q3（Brassica. napus L.）与5种十字花科杂草的杂交亲和性.结果表明，遏蓝菜（Thlaspi arvense L.）、荠菜［Capsella bursa-pastoris （L.） Medic］、碎米荠（Cardamine hirsuta L.）、风花菜［Rorippa palustris （L.） Besser］（作母本）与Q3（作父本）杂交高度不亲和.荧光显微镜观察发现花粉管生长受阻，分别停滞在上述杂草的柱头表面或花柱上部1/3处；而野芥菜×Q3则表现出一定程度的亲和性，亲和指数为1.65.在大田邻近间隔种植和自然授粉条件下，Q3与遏蓝菜、荠菜、碎米荠和风花菜间的基因流频率为0，与野芥菜间的频率为0.86%.上述结果表明转抗除草剂基因油菜与十字花科杂草间存在不同程度的基因流，基因流频率与杂交亲和性高度相关.     

Functional characterization of cadmium-responsive garlic gene AsMT2b： A new member of metallothionein family

METALLOTHIONEINS(MT)ARE LOW-MOLECULAR-WEIGHT AND CYSTEINE-RICH PROTEINS.THE FIRST MT PROTEIN WAS ISOLATED FROM EQUINE RENAL CORTEX IN1957[1].GENES EN-CODING MTS OCCUR IN DIVERSE ORGANISMS SUCH AS ANIMALS,HIGHER PLANTS,EUKARYOTIC MICROORGANISMS AND IN SOME…     

Rational design of glycerol dehydratase： Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties

1,3-propanediol (1,3-PD) is an important material for chemical industry,and there has been always much interest in the production of 1,3-PD using all possible routes. The genes encoding glyc-erol dehydratase (GDHt) from Citrobacter freundii,Klebsiella pneumoniae and metagenome were cloned and expressed in E. coli. All glycerol dehy-dratases but the one from metagenome could be detected to show enzyme activities. In order to im-prove the enzymatic properties of GDHts,the genes encoding α and β-γ subunits were cloned,and the enzyme characteristics were evolved by rational de-sign based on their 3D structures which were con-structed by homology modeling. Six heteroenzymes were obtained by swapping the α subunit genes of these three different-source-derived GDHts. The pH,thermal stability and Vmax of some heteroenzymes were dramatically improved by 2―5 times compared with the wild one (GDHtKP). The GDHt cloned from metagenome,originally proved to be with no enzyme activity,was converted into active enzyme by swap-ping its subunits with other different GDHts. In addi-tion,the effect of subtle 3D structural changes on the properties of the enzyme was also observed.     

背向滑步推铅球"过早转肩"的控制和纠正

背向滑步推铅球左侧技术中的“过早转肩”是公认的易犯错误动作之一。就多年来田径教学和业余田径训练的经验，阐述和分析了“过早转肩”的界定和危害，并提出了几点控制和纠正背向滑步推铅球“欲速则不迭”的转肩动作的教学方法及注意事项。