酶水解麦糟蛋白制备可溶性肽工艺研究

对碱性蛋白酶水解麦糟蛋白制备多肽的工艺条件进行了研究。通过单因素实验和正交实验,确定了较佳工艺条件：酶解pH10,加酶量3500u/g,酶解温度50℃,酶解时间20min。在此条件下,麦糟蛋白的水解度（DH）达22.18%,氮溶指数（NSI）达23.68%。     

不同能量水平对蓝孔雀生长速度及繁殖性能的影响

采用单因素3水平设计方法对蓝孔雀进行饲养试验,研究不同能量水平对蓝孔雀生长速度及繁殖性能的影响。试验选取2月龄蓝孔雀肉鸟90只,种鸟45只,各分为3个组,每组设3个重复,以能量水平为试验因子,肉鸟日粮代谢能水平为：11．09MJ／kg、11．53MJ／kg和12．24MJ／kg,种鸟日粮代谢能水平为11．33MJ／kg、12．21MJ／kg和12．85MJ／kg,饲养期为5个月。结果表明,肉鸟的日粮代谢能为11．53MJ／kg时其生长性能表现最佳,种鸟日粮代谢能为11．21MJ／kg时其产蛋性能最好。     

巨大芽孢杆菌AP25内切葡聚糖酶基因的克隆及序列测定

从土壤中分离到一株产纤维素酶的巨大芽孢杆菌AP25,经羧甲基纤维素平板检测,该菌可产生葡聚糖内切酶。根据GenBank登录的β-1,4内切葡聚糖酶基因（DQ782954．1,M28332．1,AY859492．1）的同源性序列,利用PCR方法克隆到该酶的基因,并对其进行测序。测序结果显示其全长为1500bp,推测其含499个氨基酸。与GenBank中的已知葡聚糖内切酶相比较,发现该酶的氨基酸序列与Bacillus subtilis的β-1,4内切葡聚糖酶基因同源性达到达94％。     

Conformation-activity studies on the interaction of berberine with acetylcholinesterase:Physical chemistry approach

Berberine has been reported as an acetylcholinesterase (AChE) inhibitor.With significantly low cytotoxicity,berberine will be developed for the clinical treatment of Alzheimer disease (AD) with higher efficacy and fewer side effects.This work investigated the structure change events of AChE that occur during the interaction with berberine by isothermal titration calorimetry (ITC),fluorescence titration,and circular dichroism (CD).The results show that the binding of berberine to AChE is mainly driven by a favorable entropy increase with a less weak affinity.Berberine causes a loss in enzymatic activity at a concentration much below the concentration which gradually exposed the tryptophan residues to a more hydrophilic environment and unfolded the protein,which indicates that the inhibition of AChE with berberine includes the main contributions of interaction and minor conformation change of the protein induced by the alkaloid.     

Comparative proteomic analysis of the response in resistant and susceptible maize inbred lines to infection by Curvularia lunata

Proteins differentially expressed from maize leaves in response to the infection by Curvularia lunata strain CX-3 were identified through a high-resolution two-dimensional gel electrophoresis (2-DE) method. Two inbred lines, 78599-1 and E28, were used, respectively, as resistant and susceptible lines to CX-3 infection. Proteins were extracted from the fourth leaves of six- or seven-leaf stage plants sampled at 24, 36, 48, 60, and 72 h after inoculation with CX-3. Twenty-seven differentially expressed protein spots resolved on the 2-DE gels were identified by MALDI-TOF MS/MS. The results showed that these proteins are associated with photosynthesis, respiration,oxidative and drought stress tolerance as well as signal transduction in maize. Among stress-related proteins, the 22 kDa drought-inducible protein, putative glutathione peroxidase (GPX), and translation initiation factor (eIF-5A) were up-regulated in the resistant inbred line and were implicated in host defense response to C. lunata infection. It suggests that drought-inducible and oxidation stress-related proteins might directly contribute to maize resistance to C. lunata.     

珙桐与光叶珙桐过氧化氢酶活性及酶谱研究

采用铁氢化钾染色法对珙桐和光叶珙桐进行CAT酶谱分析;用碘量法进行CAT酶活性的研究.研究结果表明:珙桐有酶带两条,Rf值分别为0.74和0.90,CAT酶活性为0.40824mg·(g·min)-1;光叶珙桐也有两条酶带,Rf值分别为0.76和0.93,CAT酶活性为1.3608mg·(g·min)-1;表明珙桐和光叶珙桐在遗传特性、生物进化等方面存在差异性;光叶珙桐代谢比珙桐旺盛,先叶珙桐是珙桐的变种,抗逆性更强,为珙桐种质生化分析、植物学分类及品种选育奠定了理论基础.     

江苏省实验动物笼器具 代谢笼地方标准编制说明

药物代谢动力学实验主要研究药物在动物体内动态变化的规律及特点,为临床用药提供合理剂量,在进行动物动物体内药物代谢试验时,均需应用实验动物笼器具代谢笼。代谢笼质量将会直接影响动物实验的准确性,该产品国家未颁布相关标准,江苏省是该产品生产大省,约占国内药物代谢动力学研究领域试验80％以上,因该产品至今无标准,药物代谢动力学研究领域反映强烈,迫切要求相关标准尽快制定。按江苏省质量技术监督局苏质技监标发[2007]202号“关于下达2007年度江苏省工业地方标准制定项目计划的通知”,     

隔离器与代谢笼地方标准通过国家质监总局备案审查

2008年以江苏省实验动物质量检测二站为主,联合江苏省实验动物管理委员会办公室、江苏省实验动物协会、江苏省食品药品检验所起草的实验动物笼器具代谢笼及隔离器两个地方标准,近日通过了国家质量监督检验检疫总局备案审查（审查备案号：23434—2008,23433—2008）,现由江苏省质量技术监督局正式批准实施。     

镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析

以4种辣椒为材料,分析在不同胁迫浓度和胁迫时间镉离子胁迫下,4种辣椒超氧化物歧化酶(SOD)活性及同工酶酶谱的变化.结果表明,镉离子能显著影响酶活性的变化,不同镉离子浓度胁迫下不同材料酶活性变化不同, SOD酶活性最高为对照组的1.4倍(1号品种,100 mg/L胁迫3 d),酶活性最低的为对照组的60%(2号品种,150 mg/L胁迫6 d);镉离子还能影响同工酶酶谱,不同胁迫时间和浓度对同工酶的影响不同,对1号材料的影响最为显著,能诱导出2条SOD同工酶新谱带.     

硼、钼、硅对草坪草海滨雀稗CAT和POD活性的影响

以暖季型草坪草海滨雀稗（Paspalum vaginatum Swarfz）为实验材料,通过硼、钼、硅及其配合施用,测定冬季和春季过氧化氢酶（CAT）和过氧化物酶（POD）的活性,探讨硼、钼、硅对草坪草抗寒性的作用．在冬季低温时草坪草海滨雀稗CAT活性下降．施钼和硼钼配施显著提高了草坪草海滨雀稗CAT活性．春季施钼和施硅草坪草海滨雀稗活性显著高于其它处理．在冬季低温时草坪草海滨雀稗POD活性显著升高．在春季和冬季施硅均显著提高草坪草海滨雀稗POD活性．且冬季和春季没有差异．研究结果表明：Mo和Si促进了细胞保护酶活性提高,能有效提高对生物体活性氧的清除能力．减少活性氧对生物膜的破坏作用,从而提高草坪草抗寒性．