六种中草药添加剂对离体小肠葡萄糖吸收影响的比较

就催乳保健I号、催乳保健Ⅲ号、增蛋散、止痢散、止囊散、促孕散对小白鼠离体小肠葡萄糖吸收的影响进行了比较研究。结果表明：催乳保健I号、催乳保健Ⅲ号和增蛋散对小肠葡萄糖吸收有显著的增强作用(P＜0．01),止痢散、止囊散和促孕散对小肠葡萄糖的吸收均有所增加,但未达到显著水平(P＞0．05)。催乳保健I号对小肠葡萄糖吸收的增强作用明显高于止囊散、止痢散和促孕散(P＜0．05),催乳保健Ⅲ号明显高于止痢散和促孕散,催乳保健I号、催乳保健Ⅲ号和增蛋散之间没有显著差异。     

Effects of breakfast with different calorigenic amounts on blood glucose, insulin and glucagon levels

This study was aimed to investigate the relationship between breakfast and serum glucose, insulin and glucagon concentrations in order to establish a model breakfast appropriate for Chinese. Twenty-four volunteers were randomly assigned to four study groups: high carbohydrate breakfast, high fat and protein breakfast, the typical breakfast and fasting. Each subject had serum and urine samples collected while fasting and at 1,2 and 3.5 hours following the meal. The concentration of serum glucose, insulin and glucagon was measured. The levels of serum glucose in group A, B and C differed significantly at 1 and 2 hour after meal compared to those at fasting (P<0.05). The serum glucose in group A increased insignificantly after meal. The serum insulin levels were in group A, B and C significant different compared with control group(P<0.05).Those peaked at 1 hour after meal, with group C rising the furthest. Compared with the fasting group, the serum glucagons rose and maintained the increase after breakfast in group A, B and C (P<0.05). The data suggested that various diets with different calorigenic amounts increased hormone concentration to various extents. We found that a breakfast rich in carbohydrates could maintain proper blood glucose level.     

封闭式光生物反应器集胞藻6803光自养和

采用封闭式光生物反应器进行了蓝藻基因工程常用宿主系统集胞藻Synechocystissp.PCC6803的混合营养培养,并与光自养培养进行了比较.在两种培养方式下,集胞藻6803的饱和光强基本相同,都为5000lx.当入射光强为5000lx、初始葡萄糖浓度为1.74g/L时,混合营养生长在葡萄糖消耗完(69.5h)时的藻细胞密度为1.36g/L、叶绿素浓度为20.08mg/L、能量得率为16.7%,分别为同期光自养生长的3.8倍、2.3倍和2.6倍.这表明封闭式光生物反应器混合营养培养方式在促进集胞藻6803生长和光合色素合成及提高培养过程能量得率等方面都有显著作用.     

三氯化钛活化氧化铝为载体固定化根霉葡萄糖淀粉酶的研究

用TiCl_3活化氧化铝为载体固定根霉葡萄糖淀粉酶,固定化酶的活力回收达35％、淀粉转化为右旋糖的DE值达97％以上,半衰期为433.6小时。其风干粉剂在4℃可贮存三个月以上、酵活保持不变。同时,固定化酶的最适pH范围,热稳定性和抗蛋白变性剂的能力均优于溶液酶。     

葡萄糖酶电极在酶法生产葡萄糖工业中的应用

本实验用葡萄糖酶电极测定了淀粉糖化过程中葡萄糖含量的变化，该方法快速，准确，对酶法淀粉制糖工业具有重要的意义。     

茵陈等六种中药对肝葡萄糖醛酸转移酶的体外诱导作用

为了解中药治疗新生儿黄疸是否通过酶的诱导作用，用洋地黄皂甙作为体外肝葡萄糖醛酸转移酶（ＵＧＴ）诱导的阳性对照物。用Ｂｌａｃｋ法测定茵陈（Ａｒｔｅｍｉｓｉａｃａｐｉｌｌａｒｉｓ）、黄连（Ｃｏｐｔｉｓｃｈｉｎｅｎｓｉｓ）、大黄（Ｒｈｅｕｍｏｆｆｉｃｉｎａｌｅ）、黄芩（Ｓｃｕｔｅｌｌａｒｉａｂａｉｃａｌｅｎｓｉｓ）、栀子（Ｆｒｕｃｔｕｓｇａｒｄｅｎｉａｅ）及甘草（Ｇｌｙｃｙｒｒｈｉｚａｇｌａｂｒａ）的水提物对Ｗｉｓｔａｒ大鼠肝匀浆在体外ＵＧＴ酶诱导作用。结果表明，在体外实验，这６种中药水提取物不能增加大鼠肝细胞中ＵＧＴ的活性。     

维生素C对葡萄糖磷酸化反应的影响

糖的酵解过程中,首先要经过一系列磷酸化反应,生成各种磷酸酯。其磷酸化反应的速率和量的变化,是能影响着整个酵解的进程。过去有关学者对糖酵解过程中一些关键性的酶催化机理,对于脱氢氧化反应以及辅酶I参与传递氢的作用等,均有深入细致的研究。但是,维生素C作为一种因素,能对糖的磷酸化反应有影响,以及它在糖的脱氢氧化过程中参与传递氢的作用等问题,国内外对这方面的报导较鲜见。本文现就这方面进行初步探讨,对了解维生素C在糖代谢中的作用问题,有重要意义。