量子点荧光探针的合成

量子点具有宽的激发光谱，窄的发射光谱，高的荧光量子产率，比有机染料寿命更长等优越的荧光特性，是一种理想的荧光探针，本文评述了量子点荧光探针合成的最新进展。     

水稻精细胞基因RSSG58启动子的克隆分析及表达载体构建

根据分布的水稻基因组测序的比较和水稻精细胞优势表达的RSSG58基因cDNA序列，以水稻品种“桂朝2号”黄化苗组DNA为模板，用PCR方法克隆出RSSG58在起始密码子以前的上游调控序列Pr58，经过Pr58启动子进行的鉴定和分析表明，具备大多数高等植物启动子的保守元件，预计它的RSSG58基因在特异表达方面具有一定的作用。为了鉴定RSSG58基因的基本启动子元件，将RSSG58基因5′侧翼序列做缺失片段分析，由PCR从Pr58中得以3个不同大小两端带有HindⅢ，BamHⅠ酶切位点的片段Pr58Ⅰ，Pr58Ⅱ和Pr58Ⅲ，定向插入载体pMGFP4(pBI221改建，报告基因为GFP）中，取代原有的CaMV35S启动子，构建了由驱动报造基因GFP的植物表达载体pRGFPⅠ，pRGFPⅡ和pRGFPⅢ，用于农杆菌介导法水稻遗传转化。其目的是为进一步在模式植物中研究其表达功能奠定基础。     

二苯并四氧二氮环醚及其稀土配合物合成与荧光性质研究

合成了二十员大环双氮杂冠醚：2，3，l6，l7—二苯—l，8，ll，18—四氧—5，14—二氮杂环二十烷及其7个与稀土硝酸盐(RE^3 ＝La^3 ，Sm^3 ，Eu^3 ，Gd^3 ，Tb^3 ，Dy^3 。Yb^3 )所形成的1：2(M：L)型固体配合物，并经过红外光谱、元素分析、摩尔电导及热分析等方法对配合物进行了表征．结果表明，配位是通过配体中的C—O-C进行的，而Ar—O-C未参与配位，同时含2分子配位水．对Sm(Ⅲ)，Eu(Ⅲ)，Tb(Ⅲ)，Dy(Ⅲ)的配合物进行了荧光光谱测定，所有配合物均有较强的荧光强度，其中Tb(Ⅲ)的配合物荧光强度最高，表明该新双氮杂冠醚配体三重态的能量与Tb(Ⅲ)的振动能级最为匹配．     

低温荧光摄影技术的进一步探索

低温荧光摄影技术在物证检验中的应用具有非常广阔的前景。本文进一步探索了物质的荧光特性及其研究方法，低温荧光摄影的类型及其吸收滤光技术。     

Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes

IntroductionPhotosystem II ( PSII) is a pigment- proteincomplex in the thylakoid membrane. Its reactioncenter( PSII- RC) ,which is composed of D1 andD2 proteins,generates the highly positive oxidantrequired for the oxidation of water by light- drivencharge separation. Water oxidation occurs at theMn4cluster positioned atthe center of the oxygen-evolving complex on the lumenal surface of PSII.In green plants,the highly reactive Mn4cluster isshielded by a number of extrinsic proteins( 33…     

氧氟沙星荧光光度法测定痕量铁

研究了在HAc-NaAc缓冲溶液中氧氟沙星与Fe(Ⅲ)的络合反应，结果表明：在pH=4．0的缓冲溶液中，氧氟沙星有稳定而较强的荧光；与Fe(Ⅲ)络合后，荧光强度减弱．最大激发及发射波长为324nm和498．6nm，Fe(Ⅲ)的质量浓度在0．56～3．08mg／L范围内，荧光强度与浓度呈良好的线性关系．检出限为0，076mg／L，常见的共存离子不干扰其测定．本方法用于水中痕量铁的测定，得到了满意的结果。     

苦杏仁酸-EDTA-CTMAB体系荧光法测定铽

研究了铽-苦杏仁酸-EDTA-CTMAB体系的荧光性质,探讨了荧光体系的组成及影响因素,建立了在该体系中测定铽的新方法.在pH值为12.6时,由于表面活性剂CTMAB的加入,使体系的相对荧光强度有较大的增强.在激发波长245 nm和荧光波长547 nm处测定铽,检测限达10 ng/ml.此法应用于合成样品中微量铽的测定,结果令人满意.     

N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Y^3＋,La^3＋对CaSO4：Eu^2＋发光性质的影响

用沉淀法合成了掺杂Y~(3 ),La~(3 )的CaSO_4:Eu~(2 )稀土发光材料,研究了材料的晶体结构和荧光光谱,根据实验结果,所制样品基本上属正交晶系、C_(2v)空间群,Eu离子在CaSO_4中的激发峰为基态到4f~65d(E_g),(T_(2g))激发态的跃迁;样品发射主峰位于383um,属于Eu~(2 )的~5P_(7/2)→~8S_(2/7)跃迁,说明样品中Eu离子主要以Eu~(2 )的形式存在。CaSO_4:RE(La,Y)本身没有荧光,Y~(3 ),La~(3 )掺入CaSO_4:Eu中未改变基质的晶型结构,但可增强CaSO_4:Eu中Eu~(2 )的荧光强度。