荧光猝灭法测定铕

研究了不同条件下Eu^3 与色氨酸的荧光光谱性质，实验发现Eu^3 对色氨酸的自体荧光具有显著的猝灭作用，结果表明，在pH 10．6的三酸缓冲介质中，色氨酸的荧光猝灭程度与Eu^3 浓度呈良好的线性关系，据此建立了荧光猝灭法测定Eu^3 的新方法．该方法用于样品测定，操作简便、重现性好、灵敏度高．     

一个特殊的顺式发夹核酶对感染烟草原生质体的影响

研究一个烟草花叶病毒(TMV)cDNA3′末端被特异切割的发夹核酶对感染原生质体的作用。选用一个外源基因绿色水母荧光蛋白(GFPuv)作为报道基因，取代了大部分的CP编码区域，以研究在烟草原生质体中发夹核酶对TMV载体的复制，通过GFP的表达来确定表达量。顺式发夹核酶对TMV载体感染效率的影响通过体外转录来观察。TMV GFPRIB体外转录感染原生质体的效率比与其不含该核酶的对应物的感染效率高3～5倍。Northern杂交结果表明，包括核酶在内的3’末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。当直接用载体DNA pSTMVGFPRIB感染原生质体时，感染原生质体的数量比与之相对应的pSTMVGFP NOS感染原生质体数多50％～80％。     

二氯邻菲咯啉合钴与DNA的相互作用研究

合成并研究了二氯邻菲咯啉合钴化合物与 DNA的相互作用 .通过元素分析 ,红外 ,紫外对其进行表征 ,应用荧光光谱、紫外光谱研究了配合物与小牛胸腺 DNA的主要结合方式 ,得出该配合物与 DNA的键合常数为2 .4× 10 4 ,凝胶电泳实验表明该配合物是一种简单高效的 DNA氧化切割试剂     

2,2′-环氧-1,1′-联萘-6,6′-二磺酸的合成及其光学性质

1,1′-联-2-萘酚与浓硫酸共热,以61%的产率合成了2,2,-环氧-1,1,-联萘-6,6,-二磺酸,并测定了其在不同条件下的光学性质,Stokes位移和量子效率.结果表明,荧光强度随溶剂极性的增大而增大;在pH＞6时,荧光强度受酸度影响不大,但pH＜6时,荧光强度受酸度影响较大;还研究了各种金属离子对其荧光性质的影响,其中Fe3 能产生荧光猝灭,而且在一定的浓度范围内荧光强度与Fe3 浓度成线性关系.     

用AAO模板及脉冲激光溅射制备量子点阵

 利用多孔阳极氧化铝(anodic aluminum oxide,AAO)作为模板,采用准分子脉冲激光溅射方法(pulsed laser deposition,PLD)成功制备了光荧光材料La_0.95Eu_0.05BaB₉O₁₆的量子点阵,用扫描电镜表征了AAO模板和制备的量子点阵,测量了靶材和量子点阵的光荧光谱.讨论了用此方法制备的量子点阵的结构和发光特性.证明此方法可为未来制备其它材料的量子点阵开辟了一条途径.     

Approaches to prediction of impact of Qinghai-Tibet Railway construction on alpine ecosystems alongside and its recovery

With the aid of the Remote Sensing (RS) and Geographic Information System (GIS) technology, the ecosystem pattern and fragility distribution maps of the 50-km-wide zone along the Qinghai-Tibet Railway were compiled and by using the superimposition method, range, area and indexes of the impact of various engineering activities on the ecosystems alongside the railway were studied. By making reference to the ecosystem recovery process of the Qinghai-Tibet Highway, mechanisms of recovery of the alpine ecosystems alongside the Qinghai-Tibet Railway were studied and extents and rates of the recovery were predicted.The results indicate that the impact of the railway engineering on the Alpine ecosystem depends mainly on how much the original surface soil in the zone has been disturbed and how fragile of the ecosystem per se. Restoration of vegetation coverage and species abundance shows a significantly reverse relationship with disturbance of the original surface soil but an extremely positive one with the length of the restoration period and mean annual precipitation and annual mean relative humidity in the period and no obvious bearings with altitude and temperature. In sections with an annual precipitation over 200mm, as long as a certain percentage of original soil is left in situ, it takes only 30 years or so for biodiversity to get basically restored to the original level after the construction is completed but at least 45-60 years or more for vegetation coverage.     

Changes in permafrost ecosystem under the influences of human engineering activities and its enlightenment to railway construction

~~disturbedhigh-coldmeadowarea,theoriginalvegetationisverydifficulttorestorealthoughitssurfacelandscapecanberestoredtoacertaindegree.Borrowpitsandpilinggroundshavegreatdestructiontothehigh-coldmeadow,thehigh-coldmeadowecosystemalmostcannotbere-storedbynaturalprocesses,therefore,greateffortsshouldbemadetoavoidsettingupborrowgroundinthehigh-coldmeadowregion.AcknowledgementsThisworkwassupportedbytheNationalNaturalScienceFoundationofChina(GrantNos.90102001and30270255)andtheKeyProjectionofKnowl…     

掺Pr3+钛酸盐红色长余辉发光性质比较

通过荧光光谱和磷光衰减曲线比较了CaTiO3∶Pr3+(CaTP),Ca0.8Zn0.2TiO3∶Pr3+ (CaZnTP)和Ca2Zn4Ti15O36Pr3+(Ca2Zn4TP)掺Pr3+钛酸盐的红色长余辉发光性质.测定了它们的热释光曲线.CaTP,CaZnTP和Ca2Zn4TP的热释光峰值分别是49 ℃,62 ℃和75 ℃,热释峰相对强度分别是220,186和1 254,这表明掺Pr3+钛酸盐晶体中的缺陷陷阱能级深度为Ca2Zn4TP>CaZnTP>CaTP,陷阱中电子的逸出速率是CaZnTP>CTP>Ca2Zn4TP.从理论上解释了余辉时间顺序为Ca2Zn4TP > CaZnTP > CaTP,起始发光强度顺序为CaZnTP>CTP>Ca2Zn4TP的变化规律.     

堵塞恢复时间随机的在线加拿大旅行者问题

针对旅行者在行走过程中遇到的某一或一系列无法预知堵塞事件的加拿大旅行者问题,考虑每个堵塞恢复时间是一个相互独立随机变量的情形,从在线问题与竞争策略的角度,给出了每个堵塞恢复时间都为均匀分布下的等待策略和贪婪策略以及相应策略下的竞争比,并对两种策略的执行效果进行了分析和比较.     

济南市南部山区采石废弃地的生态恢复探讨

在分析了济南南部山区废弃地现状及对环境的负面影响的基础上,在生态恢复理论的指导下,讨论了采石废弃地生态恢复技术途径与模式.指出应以植被重建为恢复目标,重点是改良基质,选择耐旱、耐瘠、速生、生物量大的灌木与草本为先锋植物种类,并结合当地条件,种植不同特性的植物.