Effect of leptin on oocyte maturation and subsequent pregnancy rate of cloned embryos reconstructed by somatic cell nuclear transfer in pigs

Cloning pigs by somatic cell nuclear transfer （SCNT） has wide applications in basic research, human medicine and agricultural production. To improve cloning efficiency, the effect of two basic maturation media, NCSU-23 and TCM199, was compared, and TCM199 was selected for the following experiments with leptin. We systematically studied the effects of leptin supplementation on oocytes in vitro maturation （IVM）, in vitro development of parthenogenetically activated （PA） and SCNT embryos and in vivo development of SCNT embryos after embryo transfer （ET）. The results showed that supplementation of 100 or 200 ng/ml leptin into the maturation medium did not greatly affect nuclear maturation of oocytes, or cleavage rates of PA and SCNT （P 〉 0.05）. Blastocyst rates of PA and SCNT embryos were significantly improved when 100 or 200 ng/ml leptin was added to maturation medium, and the number of cells in PA blastocysts was also improved （P 〈 0.05）. The number of cells in blastocyst of SCNT was improved, when 100 ng/ml leptin was added （P 〈 0.05）. Furthermore, supplementation of 100 or 200 ng/ml leptin to the IVM medium may improve pregnancy rate and the delivery rate inpig cloning.     

单侧唇裂和鼻畸形同期矫治的临床研究

目的：探讨单侧唇裂及鼻畸形同期矫治的有效方法。方法：用Mmard法及改良术式整复84例深Ⅱ度及Ⅲ度唇裂,全层切开裂隙缘切口,其余切至口轮匝肌表面,脱套式潜行剥离口轮匝肌,分离鼻小柱和鼻翼的异常附着,采用肌层功能复位,鼻翼软骨悬吊,使鼻畸形得到明显改善。结果：84例患者随访2周～6年,除了1例伴发严重的腭裂和牙槽嵴裂致患侧鼻翼仍有塌陷,其余均满意。结论：单侧唇裂和鼻畸形同期矫治不增加附加切口,效果肯定,能一次让鼻唇畸形得到较满意的恢复。     

“古屋”的倒塌——《万延元年足球队》中的“暴力”解构

日本文化中多有对武力的崇尚和赞誉,也正是在这样的崇尚中,他们的民族性格中充满了扩张和掠夺的欲望。这是一种原始人性的暴露,但更是被欲望扭曲的人性的表现。本文通过对大江健三郎作品《万延元年足球队》中暴力描述的分析,试图揭示出暴力与荣耀、自杀的关系,以及暴力背后人的畸形生存体验,最终在完成对日本文化暴力信仰解构的同时,提出了如何在暴力之后的废墟之上重构人性的问题。     

世界十大恐怖之地

1．美国费城马特博物馆刚建立时，该博物馆是用来供医学院的学生做人体懈剖和研究畸形人的地方。如今这里已经成为一个展出各种疾病、伤势、畸形案例，以及古代医疗器械和生物学发展的大展厅，展品逾20000件，其中包括战争中伤者的照片、连体人的遗体、侏儒的骸骨以及人体病变结肠等。     

故意杀害孕妇的若干问题探讨

目前,故意杀害孕妇致使胎儿在孕育生长过程中遭受第三人不法侵害的案件频繁发生,我国关于胎儿保护立法不完善,因此在审理故意杀害孕妇胎儿案件时定罪量刑问题存在很大争议。通过对故意杀害孕妇人数计算问题、法学界关于故意杀害孕妇行为认定的评析和对故意杀害孕妇量刑适用的探讨,并借鉴国外立法,结合我国司法实践,进而探讨对我国故意杀害孕妇立法的量刑界定。     

日本公司推出3D胎儿打印

一些准妈妈常迫不及待地想让亲友知道胎儿的模样,现在她们不用发愁了,一家日本公司推出3D胚胎模型服务满足了她们的愿望。日本一家名为FASOTEC的公司推出的这项服务有个好听的名字一一"雕刻天使"。孕妇接受核磁共振检查后,该公司可以通过3D打印技术制作胚胎模型。胎儿的样子会被     

阑尾可以随意舍弃吗

许多人得了急性阑尾炎,医生就会把阑尾切除,那么人的阑尾真的可以随意舍弃吗?     

Rapid diagnosis with FISH for chromosomal abnormality of fetal pyelectasia

Fluorescence in situ Hybridization （FISH） was used to investigate whether the chromosome of the fetus prenatally diagnosed as pyelectasis was normal or not. Amniotic fluid was taken from the pregnant woman whose fetus was detected with pyelectasia by prenatal examination. The chromosome of the amniotic fluid cell without culture was examined with FISH. The result shows that compared with the traditional amniotic fluid cell culture, FISH has the advantages of more rapid, higher sensitivity and specificity, and was 10-12 days earlier to complete the diagnosing than the traditional method. The fetuses detected chromosomal abnormality in each groups were induced during the middle and late trimester, while those fetuses with normal chromosome continued pregnancy, the rate of spontaneous disappearance of pyelectasia decreased as the severity of pyelectasia increased. FISH can satisfy the urgent need in the clinical prenatal diagnosis due to its rapidity to determine whether fetus with pyelectasia was accompanied with chromosomal.     

幼儿消费观的培养

目前音乐胎教的功效、方法等在我国还没有出台严格的标；隹，尽管某些方面研究证明，音乐对刺激胎儿的发育有一定好处，但胎教时音乐、音量的选择有较大的随意性，加之整个胎儿期大脑、神经发育都非常的娇嫩，因此，准妈妈们在胎教时不要过于盲目。     

1715例蒙汉胎儿产前超声检查结果分析

对内蒙古医科大学附属医院2011年9月至2012年5月的1715例孕17-34周胎儿进行规范化产前超声检查结果分析研究,得知在1715例胎儿中,超声检查诊断胎儿畸形65例(3.79%),其中蒙族10例(0.58%),汉族54例(3.15%),其他少数民族1例(0.06%).超声软指标阳性检出96例(5.6%),蒙族16例(0.93%),汉族76例(4.43%),其他少数民族4例(0.23%).现有数据显示蒙古族胎儿畸形检出率以及软指标阳性率均较汉族胎儿低,但无统计学差异.中枢神经系统畸形、骨骼系统畸形、胃肠道系统畸形分列胎儿结构畸形前三位.心室内斑点样强回声和肾盂轻度扩张是最常见的软指标阳性,在产前诊断中需加强咨询和随访.