慢性充血性心力衰竭过程中的肿瘤坏死因子及其受体

慢性充血性心力衰竭病人血清中TNFα水平升高，当其≥10^-8mol/L时，可能作为内分泌激素，通过与其受体(膜结合型及可溶型)结合，直接损伤心肌纤维，影响心肌细胞凋亡过程，引起或促进心功能不全的发生。TNF途径成为充血心衰发病机制中的新内容。可考虑应用TNFr拮杭剂治疗心衰。     

活菌作为抗肿瘤制剂和肿瘤基因治疗载体的研究

随着细菌生物学和分子生物学的发展，细菌抗癌治疗的应用更为广泛，其可增加化疗药物的敏感性，增强抗癌药物作用，并作为基因治疗的载体，细菌毒素本身就可以用来攻克肿瘤，而最有应用前景的是，经遗传修饰的细菌作为自杀基因载体选择性地破坏肿瘤细胞，保护正常组织不受损坏。文章就活菌作为抗肿瘤制剂和肿瘤基因治疗栽体的研究进展进行综述。     

透明质酸在肿瘤新血管生成中的作用

阐述了透明质酸寡聚糖对上皮细胞作用的过程，即从与细胞表面受体结合到激活信号传导途径，以及表达成蛋白质进行细胞增生和转移。     

连续小波变换在中医脉象信号处理中的应用

小波变换具有良好的时频局域化性质，适合于非平稳信号的处理。脉诊是中医诊察疾病的重要手段，脉象反映的是人体的生理与病理信息，脉象信号具有随机性和非线性等特点。应用连续小波变换分析了15例海洛因吸毒者和15例正常人的脉象信号，提取了吸毒者脉象信号中的异常信息，为戒毒治疗的评估与改进提供客观依据。在尺度0.1、0.2、0.3和0.4下，对每一例脉象信号分别进行了处理，发现在0.2-0.4s时间间隔内，海洛因吸毒者和正常人脉象信号的连续小波变换系数间存在显著差异，并提出了用于划分海洛因吸毒者一正常人的临界参数。研究结果表明，连续小波变换是处理脉象信号的有效方法。     

循证医学对肿瘤临床实践的指导意义

介绍循证医学产生的背景及其对肿瘤临床的实践意义，肿瘤学循证医学文献检索的几种主要的数据库。     

Advances in identification and application of tumor antigen inducing anti-cancer responses

Tumor antigen is one of the important bases of tumor immunotherapy . With the discovery of novel tumor antigens, interest in specific immunotherapy for treatment of malignancies has increased substantially. Nowadays more and more scientists paid close attention to various tumor antigens with their roles or/and applications in anti-cancer immune responses, immune tolerance, tumor markers,     

Classification of Medical Brain Images

Since brain tumors endanger people‘s living quality and even their lives, the accuracy of classification becomes more important. Conventional classifying techniques are used to deal with thosedatasets with characters and numbers. It is difficult, however, to apply them to datasets that include brain images and medical history (alphanumeric data), especially to guarantee the accuracy.For these datasets, this paper combines the knowledge of medical field and improves the traditional decision tree. The new classification algorithm with the direction of the medical knowledge not only adds the interaction with the doctors, but also enhances the quality of classification. The algorithm has been used on real brain CT images and a precious rule has been gained from the experiments. This oaoer shows that the ahzorithm works well for real CT data.     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

海洋生物活性物质在肿瘤治疗及临床检测中的应用

海洋是地球生命的摇篮。浩瀚的海洋是一个巨大的资源宝库,又是一个潜力巨大的天然药源。由于海洋对人类生存和可持续发展具有重要意义,21世纪将成为海洋的世纪。 在5.1亿平方公里地球总面积中,海洋占71％。     

人肾癌TIL细胞抗原受体Vβ基因的优势表达

定量聚合酶链反应(qPCR)技术的应用有力地推动了对T细胞抗原受体谱(TCR reper- toire)的研究,已能够以此分析TCR α和β链编码基因V区和J区片段限制性取用(Re-stricted usage)的格局,确定抗原驱动下发生寡克隆扩增的T细胞及其特点,有可能为肿瘤特异性免疫干预开拓新的前景.有关尝试已在人体黑色素瘤、肝细胞癌中获得结果.由于T细胞识别的是抗原肽和MHC分子构成的复合物,以往的研究往往忽略了肿瘤浸润淋巴细胞(TIL)供者的HLA背景,致使结果不一致.本研究采用qPCR技术分析人体肾细胞癌取用TCR Vβ格局的同时,测定肿瘤细胞表面HLA Ⅰ类抗原表达状况,并作HLA分型.现报告对3例患者的分析结果.