提高灰黄霉素产生菌Penicillium patulumF—208孢子萌发率的生化…

添加２３种不同的生化单因子及由其组合成的复合生化因子于种子培养液中进行摇瓶培养，甘氨酸等生化单因子对Ｐｅｎｉｃｉｌｌｉｕｍ ｐａｔｕｌｕｍＦ２０８孢子的萌发有促进作用，而以甘氨酸＋Ｌ－组氨酸＋Ｌ－赖氨酸＋嘌呤＋－Ｌ－酷氨酸组成的复合因子的作用更为显著，孢子萌发率由的５５．５％提高到９３．５％，复合生化因子的添加对移种期蛋白酶和淀粉酶的酶活有一定的影响，而对发酵过程中的上述酶活性无明显影响。     

艾维菌肉鸡在石河子垦区的饲养观察

<正>垦区饲养肉鸡已有八个年头,在品种上从塔特姆、明星、罗曼、罗斯、AA到艾维菌,养得得心应手的属艾维菌,养得最好、最成功的是1993年和1994年,现将艾维菌肉鸡在我区的饲养管理,疫病防治、生产性能等方面的实验观察作一总结,供同行参考.鸡舍的清洁卫生鸡舍的清洁卫生是进雏前的一项重要工作,一定要做好做细.1、把鸡只由鸡舍内全部清出去.2、可移动的设备(漏粪竹架板和塑料网、料槽、饮水设备、产蛋箱等)从鸡舍移出.     

紫膜Langmuir-Blodgett膜中菌紫质的光电压

80年代生物分子电子学和生物计算机的研究蓬勃兴起,嗜盐菌紫膜蛋白菌紫质由于其结构稳定,具有光驱动质子泵功能、双稳态特性以及皮秒到毫秒级的光电响应特性,使其成为目前国内外关注的研究生物分子电子器件的理想材料之一,应用前途是发展生物分子器件和生物芯片等.由于紫膜具有不对称性而菌紫质的光驱动质子泵具有方向性,因而必须有序组装而且可以有序组装.紫膜Langmuir-Blodgett(LB)膜是这种有序     

白粉菌分类中无性型特征的研究

探讨了白粉菌无性型特征,尤其是分生孢子形态、纤维体的显著与否、芽管形态及着生部位等形状在白粉菌分类中的作用.描述了 Arthrocladiella mougeotii(Lev.)Vassik.等6属7种白粉菌的无性型特征.结果表明,在缺乏有性型或完全采用有性型易与其它种混淆时,综合无性型特征进行白粉菌分类是完全可行的.     

34株生孢囊放线菌的分类研究

本文对从云南地区分离到的34株生抱囊放线菌进行了形态、培养特征,全细胞壁化学组分的分析,并据其孢囊着生的位置、形状;细胞壁的化学组分将它们初步归于游动放线菌属(Actinoplanes)和链孢囊菌属(Streptosporangium)。依其在五种培养基上的培养特征将其作了初步的分组。     

Cloning and analysis of the antagonistic related genes of <Emphasis Type="Italic">Enterobacter cloacae</Emphasis> B8

To understand the antagonistic mechanism of the broad spectrum antagonistic Enterobacter cloacae B8,Tn5 transposon-mediated mutagenesis is performed using suicide plasmid pZJ25. Two mutant strains that lost antagonistic character are isolated. Tagging with kanr gene on Tn5,an antagonistic related DNA fragment, the F fragment, right of the Tn5 insertion site is cloned in a plasmid named pTLF,from one of the mutant strains B8F. The 733 bp F fragment is then sequenced after subcloning. Genomic DNA of the original B8 strain is isolated, digested with Pst I and ligated to Pst I cassette. DNA fragments left and right of the F fragment are amplified from the Pst I cassette library using cassette primer and specific primers designed according to known sequence. 1106 bp sequence left of the F fragment and 664bp sequence right of the F fragment are finally obtained. Bioinformatics analysis shows that the contig assembled from the sequences of the cloned antagonistic related DNA fragments of B8 encodes three ORFs and is homogeneous to admM,admN and admO genes of Pantoea agglomerans andrimid biosynthetic gene cluster (AY192157). The ORF, named anrF gene which encodes a polyketide synthase, knocked out by Tn5 insertion, is a homology of admM and the insertion site of Tn5 is at 214 bp upstream of the stop codon. It is concluded that the anrF gene is a gene related to the antagonistic activity of E. cloacae B8, and speculated that the antagonistic substance produced by B8 is an andrimid.     

Ovicidal activity of two fungal pathogens （Hyphomycetes）against Tetranychus cinnabarinus （Acarina：Tetranichidae）

The carmine spider mite, Tetranychus cinna-barinus, is an economically important pest that devastates varieties of crops worldwide and develops significant resistance to common chemical pesticides, most of which lack ovicidal activity. In the present study, two isolates of entomopathogenic fungi, Beuaveria bassiana SG8702 and Pae-cilomyces fumosoroseus Pfrl53, were bioassayed against T. cinnabarinus eggs at 25 ℃ under a photophase of 12 : 12 (L:D). Infected eggs on Vicia faba var. minor leaves failed to hatch due to distortion and shrinkage and had fungal outgrowths when maintained under moist conditions. Sprays of B. bassiana conidia to T. cinnabarinus eggs (on leaves) at the concentrations of 58, 298 and 1306 conidia/mm2 (3 replicates per concentration and 35-65 fresh mite eggs per replicate) resulted in corrected egg mortalities of 20.4±4.2%, 36.0±7.6% and 64.6±12.5% (F=43.14, P <0.01), respectively; sprays of P. fumosoroseus at 129, 402 and 2328 conidia/mm2 caused egg mortalities of 16.1±11.1%,     

TiO_2/GAC光催化剂对中水杀菌效果的研究

探讨了利用太阳光作光源,固定在颗粒活性炭上的二氧化钛光催化剂(T iO2/GAC)对中水除菌效果的影响因素.实验表明细菌的浓度范围在5.7×107~5.7×102cfu/mL时,光催化除菌率为86.1%~99.5%.光照强度在1.35 mW/cm2时,除菌率达96.6%.光照加T iO2/GAC 3 h时,99%以上的菌体死亡.仅光照无T iO2/GAC时除菌效果甚微,只有15.4%的菌体失活;仅有T iO2/GAC无光照,除菌率为42.3%.     

酒色着色菌Chromatium vinosum膜结合态氢酶基因hupSL的克隆及分析

光合细菌酒色着色菌(Chromatiumvinosum)含有Hup膜结合态氢酶.根据同科的桃红荚硫菌(Thiocapsaroseopersicina)Hup氢酶同源序列,在结构基因小亚基hups和hupC的保守框上设计1对简并引物:Ps:5’CCGACCAC(C/G)TACAACGCCTG3’和Pc:5’CG(G/C)GACATGATGTC(T/C)TCGCG3’.通过PCR扩增获得2.6kb的扩增片段,克隆后进行序列分析.结果表明,该片段包含部分的小亚基hupS序列、大亚基hupL的全序列及hupC部分序列.从已知的hupS序列选取适当位点合成引物AS2:5’CTACGACCATGTCACCGACA3’,并利用接头寡核苷酸序列P6:5’CCTTGTGAAATTGTTATCCGCT3’,通过PCR扩增获得1.2kb的扩增片段,克隆后进行序列分析.结果表明,该片段包含完整的膜结合态氢酶的小亚基基因hupS.