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91.
张东向 《齐齐哈尔大学学报(自然科学版)》2003,19(1):92-95
就各种因素对齿瓣延胡索悬浮细胞生长和延胡索乙素形成的影响做进一步的研究。结果表明:随着接种量的提高,细胞生长加速,培养液浊度增大,齿瓣延胡索细胞悬浮液培养中最适接种量为2g/30ml;pH5.6最有利于细胞生物量的积累;2,4—D与2种细胞分裂素的组合,对于生长量积累和THP含量的作用最显著;在培养基中添加甘露糖和半乳糖,对于提高培养物THP含量是有效的;G1y Phe Lys的作用效果最显著,细胞鲜质量、干质量和THP含量均最高。 相似文献
92.
新疆杨植株再生体系的建立 总被引:16,自引:1,他引:16
从培养基筛选、激素调控等方面探索建立新疆杨高效植株再生体系。结果表明,不同种类的细胞分裂素影响叶盘不定芽分化频率,其中较低浓度的TDZ(Thidiazuron)可显提高叶盘不定芽分化频率,生长素的种类与浓度对叶盘不定芽分化影响不显。较适合新疆杨不定芽分化的培养基为:1/2MS TDZ0.005mg/L BA0.25mg/L IAA0.5mg/L;壮苗培养基为:MS BA0.2mg/L IAA0.2mg/L;生根培养基为:1/2MS。在此条件下,不定芽分化频率可达100%。 相似文献
93.
A primitive orthorhombic crystal form of acylamino acid releasing enzyme‘lipase(APE1547)from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291K.The diffraction pattern of the crystal extends to 0.27nm resolution at 100K using Cu Kαradiation.The crystal belongs to the space group P212121 with unit cell dimensions of b=6.399,b=10.439and c=16.953nm.The presence of two molecules per asymmetric unit gives a crystal volume per protein mass(Vm)of 0.0022nm^3 Da^-1 and a solvent content of 43% by volume.A full set of X-ray diffraction data were collected to 0.3nm from the native crystal. 相似文献
94.
GUOHonglian CAOQinhong RENDongtao LIUGuoqing DUANJianfa LIZhaolin ZHANGDaozhong HANXuehai 《科学通报(英文版)》2003,48(5):503-508
A 1-μm in diameter polystyrene bead coated with lectin is trapped by optical tweezers whics is formed by a focused laser beam (740nm).A leucocyte adhered to the bottom of sample cell is chosen to close to the trapped bead.When their stable combination is confirmed,the leucocyte is displaced by moving the sample stage.A“tether”is then formed between the trapped bead and the membrane.The force acting on the tether is measured by a detector equipped on the optical tweezers system.The deformation of the membrane is diverse in different contact conditions.When the contact area is small,the tether is very thin and the force on it is 7.4pN(10^-12N),while the tether is much thicker under a larger contact area and its corresponding force is about 14pN.It is presumed that the latter is related to the deformation of cytoskeleton. 相似文献
95.
研究铂(II)与三种胺类配合物的合成、晶体结构和体外抗肿瘤活性,化合物1的X-射线单晶结构分析表明:这是一个很少见的具有四方锥几何构型的铂(II)配合物,顺式平面四方形配位的铂的轴向上有第5个配位原子。其抗肿瘤活性实验显示3个配合物对几种研究的癌细胞都有较强的抑制作用。 相似文献
96.
Fractals and cellular automata are both significant areas of research in nonlinear analysis. This paper studies a class of fractals generated by cellular automata. The patterns produced by cellular automata give a special sequence of sets in Euclidean space. The corresponding limit set is shown to be a fractal and the dimension is independent of the choice of the finite initial seed. As opposed to previous works, the fractals here do not depend on the time parameter. 相似文献
97.
MAOJinshu WANGXianghui CUIYuanlu YAOKanade 《科学通报(英文版)》2003,48(17):1807-1810
With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials. 相似文献
98.
WANGNing CHENXiaoguang CHENLi YAOJihua CHENHaoming SHENQi XUEJinglun 《科学通报(英文版)》2003,48(19):2061-2063
Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor. 相似文献
99.
100.
彗星电泳检测细胞DNA损伤应用新进展 总被引:3,自引:0,他引:3
彗星电泳是近年发展起来的一种测定单个细胞DNA损伤的方法 ,因其操作简单、快速及灵敏等特点 ,应用范围十分广泛 .着重介绍了这一技术的发展、图形分析及应用进展 相似文献