细胞内信号转导的发现

20年前，当史图尔特·施利柏（StuartSchreiber）28岁时，他获得了合成美洲大螃性诱剂PeriPlanon－b自然形态第一位化学家的殊荣。施利柏说．他所以对这种性诱剂感兴趣，是由于它分子结构上的几何图形美。在耶鲁大学实验室合成了这种性诱剂分子后，他判断还能继续完成显而易见的试验，于是他搬到化学大厦的底层继续工作。一我有一车鉴别各种蟑郁的图谱．”他解释道，“但我不会区别雌雄。书上说，蟑螂腿上有标志可区别雌雄，但我从未干过。我找到一些美洲大腿，——大个儿的蟑螂，——不知其是雌是雄。但只有雄蟑螂才对美洲大螂性诱剂起反…     

三种细胞质雄性不育水稻小孢子发育过程的研究

运用ＤＮＡ荧光探针结合显微荧光术，系统地研究了３种水稻细胞质雄性不育系的败育过程，时期和特点。结果表明，珍汕９７不育系不能完成小孢子第一次有丝分裂；马协不育系小孢子可完成第一镒有丝分裂，但不能进入第二次有丝分裂；广丛４１不育系部分小孢子可完成第二次有丝分裂，因此可看到许多三核花粉，但因核行为异常而败育。     

光周期敏感细胞质雄性不育小麦的初步研究

选用属Ｄ＾２型细胞质粗厚山羊草（Ａｅｇｉｌｏｐｓｃｒａｓｓａ）、牡山羊草（Ａｅ．ｊｕｖｅｎａｌｉｓ）和瓦维洛夫山羊草（Ａｅ．ｖａｖｉｌｏｖｉｉ）分别与普通小麦（Ｔｒ．ａｅｓｔｉｖｕｍ）品种鄂恩１号、ＮＰＦＰ和白皮２２４连续回交进行核转换组配的９个异质系（即核质杂种）和核供体品种鄂恩１号、ＮＰＦＰ和白皮２２４，于分蘖始期（叶原基期）和拔节始期（小花分化期）分别置于１５．５－１６ｈ和２４ｈ长光照下，以     

Surface plasmon resonance analysis to evaluate the importance of heparin sulfate groups＇ binding with human aFGF and bFGF

Human acidic and basic fibroblast growth factors (aFGF and bFGF) are classic and well characterized members of the heparin-binding growth factor family. Heparin is generally thought to play an extremely important role in regulating aFGF and bFGF bioactivities through its strong binding with them. In order to unravel the mechanism of the interactions between heparin and FGFs, and evaluate the importance of heparin sulfate groups‘ binding with FGFs, surface plasmon resonance analyses were performed using IAsys Cuvettes System. Heparin and its regioselectively desulfated derivatives were immobilized on the cuvettes, aFGF and bFGF solutions with different concentrations were pipetted into the cuvettes and the progress of the interaction was monitored in real-time by Windows-based software, yielding kinetic and equilibrium constants for these interactions. In addition, in order to reduce the delicate difference among the cuvettes, inhibition analyses of mixture of FGFs and immobilized native heparin by modified heparins were also done. The data from these two methods were similar, indicating that all sulfate groups at 2-0, 6-0 and N- in heparin were required for the binding to aFGF; and that their contribution to the binding was in the order 2-O, N- and 6-O-sulfate group.In contrast, definite contribution of the 6-O-sulfate group to the binding with bFGF was most apparent, while the other two sulfate groups appeared to be necessary in the order 2-O and N-sulfate group. These methods established here can be used for analysing the effect of sulfate groups in heparin on the binding with other human FGF members or other heparin-binding proteins.     

小麦及其近缘种的细胞质分类

以小麦及其近缘种的３９个同核异质量换系为材料，选取３３个性状进行了Ｑ分析，结果表明：这些细胞质材料有着明显的遗传差异性，可将它们分为８个不同类型，这些细胞的遗传分化主要发生在二倍体水平上，并且在种内也存在着细胞质的分化。     

三系杂交棉新组合——浙杂2号

浙棉2号是通过分子标记辅助选配技术和质核互作不育系制种技术,于2002年配制出高优势、优质三系杂交棉新组合。2002年参加品种比较试验和浙江省棉花品种多点试验,2003～2004参加浙江省棉花品种区域试验和生产试验,2005年通过浙江省审定。该组合的细胞质不育基因和恢复基因均来源于陆地棉,克服传统三系杂交棉因来源于哈克尼西野生棉的不育基因和恢复基因而导致恢复力低和杂种优势弱等问题,使三系杂交棉产量水平有显著突破。     

利用酵母双杂交系统筛选油菜ATP6的相互作用因子

甘蓝型油菜波利马(Brassica napus pol.)细胞质雄性不育系是一种很有实用价值的油菜不育类型.目前关于其分子机制的研究已证实,线粒体ATP合成酶的一个亚基ATP6与其雄性不育相关.本实验以ATP6作为诱饵蛋白,应用酵母双杂交共转化的方法筛选与ATP6相互作用的蛋白质.在获得的阳性克隆中,发现了一个与拟南芥中未知基因AT3G47550所编码的蛋白质具有较高同源性的蛋白质.它含有RING HC的结构,可能在泛素-蛋白质降解途径与细胞质雄性不育间建立起了某种联系.