闭合性松解术加解锁法为主治疗老年膝关节骨性关节炎的临床研究

目的应用闭合性松解术加手法整骨对老年膝关节骨性关节炎患侧髌骨应用解锁法,在进行治疗同时设立对照组对比,探索此疗法的临床疗效.方法 试验组121例,应用闭合性忪解术加解锁法为主,对照组共123例,应用针灸加解锁法进行治疗,同时在试验中间点及试验终点进行观察记录有关指标.结果对照组治疗前,试验中间点及试验终点t检验p＜0.05,试验组疗效明显,优于对照组.结论针对老年膝关节骨性关节炎的发病因素(力平衡失调因素,无菌炎症因素,老年退变合并骨疏松)进行闭合性松解术加解锁法,同时针对不同症状配合必要的减压,使用降钙素等措施,能满意解决老年膝关节骨性关节炎的病变及症状,不同程度地恢复患肢功能,是一种值得推广的治疗方法.     

Involvement of a cytoplasmic glyceraldehyde-3- phosphate dehydrogenase GapC-2 in low-phosphate- induced anthocyanin accumulation in Arabidopsis

Phosphorous is one of the essential mineral elements for plant growth and development.Typically, the shoots of plant seedlings usually turn a dark-brown or purple colour under low-Pi stress. Using protein 2-D gel and peptide mass fingerprinting mapping (PMF) methods, a cytoplasmic glyceralde-hyde-3-phosphate dehydrogenase GapC-2 was identified as a low-Pi responsive protein in Arabidopsisplants. Expression of AtGapC-2 protein was significantly decreased after 4 d of low-Pi stress. Two in-dependent T-DNA insertion lines of GapC-2 gene (At1g13440) showed a hypersensitive phenotype inresponse to low-Pi stress compared with wild type plants, while the transgenic complementation linesof the mutants showed a similar phenotype to the wild type. These results indicate that AtGapC-2 mayplay an important role in Arabidopsis responses to low-Pi stress, possibly by regulation of glycoly-sis-associated "Pi-pool" and accumulation of anthocyanin pigments in plants.     

REE composition in scheelite and scheelite Sm-Nd dating for the Xuebaoding W-Sn-Be deposit in Sichuan

The scheelite with large size and euhedral shape from Xuebaoding, Sichuan Province is an ideal min- eral to study REE patterns and Sm-Nd dating of non-gold deposits. The scheelite has diameters of 1― 10 cm and colors of pale beige to deep orange. Most of these scheelites occur in association with beryl, cassiterite and muscovite. The rare earth elements indicate that scheelite samples from Xuebaoding W-Sn-Be deposits contain high concentrations of rare earth elements with total ΣREE Y contents in the range of 369―1725 μg·g?1 and nearly all of these scheelite samples are enriched in LREE and depleted in HREE and have negative Eu anomalies. The REE patterns of the scheelites are similar to those of A-type granite with obvious tetrad effect, similar to that of type II scheelites. Based on reported fluid inclusion studies, the mineralizing-fluid of the Xuebaoding W-Sn-Be deposit indicates low Na activity. The REE patterns of the scheelite are probably controlled by partition coefficient in hydrothermal fluid. In the 147Sm/144Nd-143Nd/144Nd diagram, the scheelites show a linear array corresponding to an isochron age of 182.0±9.2 Ma (MSWD = 0.52). The Sm-Nd age represents the formation age of the scheelite and can indicate that the W, Sn and Be mineralization at Xuebaoding took place in the Early Yanshanian. The Sm-Nd dating result is important for the study of the multi-metal resources in western Sichuan Province.     

金黄色葡萄球菌sPP2C的克隆、表达及性质研究

从金黄色葡萄球菌基因文库中克隆了一条新的双特异性磷酸酶，命名为sPP2C (protein phosphatase 2C, Staphylococcus aureus). sPP2C基因具有741个碱基，编码的蛋白有247个氨基酸，具有一个蛋白磷酸酶2C的催化结构域.sPP2C的分子量为26.1 kD，等电点为4.95.在E.Coli. Rossetta中表达蛋白sPP2C.高纯度的sPP2C用亲和层析的方法纯化得到.酶学研究结果表明：sPP2C对磷酸酶的通用底物硝基苯磷酸 (p-nitrophenyl　phosphate, pNPP)不起作用，而与pSer/Thr和pTyr的寡肽均有去磷酸化作用.这些实验结果说明sPP2C是一个新的双特异性磷酸酶.     

酚酞与12-钨磷酸复合物的低热固相合成、表征及性质

本文以酚酞和Keggin结构12-钨磷酸(H3PW12O40.7H2O)通过一步低热固相法,合成了一系列无机-有机复合物,用X射线粉末衍射、红外光谱、紫外-可见漫反射光谱进行了表征。结果显示,这些复合物具有几乎相同的晶相结构,更为奇特的是,这些复合物都保持了12-钨磷酸的Keggin结构。性质试验表明,这些化合物都显示出可逆的热致变色性,在极性溶剂中发生分解,无法通过液相反应合成。     

我国科学家在植物表观遗传学方面取得重要进展

植物表观遗传学是当前植物学研究领域的一个热点，在国家杰出青年科学基金和“九七三”等项目的资助下，中国农业大学巩志忠教授领导的研究小组在该方面取得了重要进展．巩志忠教授及其研究小组利用基因沉默抑制因子ROS1突变后导致35S启动子-新霉素磷酸转移酶（Pro35S：NPTⅡ）基因沉默做为筛选标记，筛选并获得解除Pro35S：NPTⅡ基因沉默的了两个等位突变体rorl-1和rorl-2（rosl突变的抑制因子）,图位克隆显示ROR1编码一个与DNA复制蛋白A2（RPA2A）相似的31-kD的蛋白。     

益气活血通络方对糖尿病肾病大鼠肾组织RAGE/NOX4/ROS信号通路及氧化应激的影响

目的:观察益气活血通络方对糖尿病肾病(DKD)大鼠模型晚期糖基化终末产物受体(RAGE)/烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX4)/活性氧(ROS)信号通路以及氧化应激的影响,探讨其治疗DKD的可能作用机制。方法:雄性SD大鼠40只随机分为正常对照组(CON)和造模组。造模组大鼠通过为期6周的高糖高脂饲料饲养,联合链脲佐菌素(每kg大鼠体质量注射35 mg链脲佐菌素)建立DKD大鼠模型,成模大鼠随机分为模型组(MOD)、益气活血通络方低剂量组(DL)、益气活血通络方高剂量组(DH)、厄贝沙坦组(IRB)进行灌胃,给药组分别灌胃给药20周,期间CON组及MOD组予等量生理盐水。给药结束后全自动分析仪检测各组大鼠尿微量白蛋白与尿肌酐比值(UACR),羟胺法检测血清锰超氧化物歧化酶(SOD2)活性,TBA法检测血清丙二醛(MDA)浓度;HE染色法、Masson染色法、PAS染色法观察各组大鼠肾组织病理形态学变化;二氢乙锭(DHE)荧光探针检测大鼠肾组织ROS表达;免疫组化法(IHC)检测大鼠肾组织RAGE、NOX4蛋白表达;Western blot法检测SOD2、RAGE、NOX4蛋白表...     

基于MSCR试验多聚磷酸改性沥青高温性能评价

【目的】探讨多聚磷酸(PPA)改性沥青的高温性能。【方法】对不同多聚磷酸掺量的改性沥青进行多应力蠕变恢复试验,并通过试验分析基质沥青和RTFOT试验后PPA改性沥青在3.2 kPa剪切应力下平均不可恢复蠕变柔量J_nr3.2值、不可恢复蠕变柔量相对差异J_nrdiff和PG高温连续分级温度HT等技术参数。【结果】试验结果表明,随着PPA掺量的增加,PPA改性沥青结合料的3.2 kPa剪切应力下平均不可恢复蠕变柔量J_nr3.2减小、不可恢复蠕变柔量相对差异J_nrdiff增大、PG高温等级提升。【结论】PPA改性沥青结合料的高温性能得到明显改善,沥青黏弹性能对于应力变化的敏感性增强。     

钙调磷酸酶B亚基钙结合位点的突变对B亚基结构和功能的影响

应用远紫外CD光谱、紫外差光谱、内源荧光光谱以及ANS荧光光谱,探究了钙调磷酸酶B亚基钙结合位点的突变对B亚基结构和功能的影响．结果显示:钙结合位点突变体Y105W激活CNA的能力强于CNB;而E110Q活化CNA的能力弱于CNB;Y105W和E110Q与Ca2+亲和性低于CNB;CNB和它的突变体二级结构趋于一致,但三级结构存在明显差异,这种差异可能是它们功能差异的结构基础．