Real time observation of the photocleavage of single DNA molecules

A method for real time observation of photo-cleavage of stratched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed.DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide.Then the Process of Photocleavage and relaxation of DNA under radiation of blue light is observed.We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules.The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity,cancer research as well as the interaction between DNA and dyes.     

生物入侵谁的黄昏

人类才是破坏生态体系的源头,是生物入侵的源头,也是破坏生物多样性的源头。 我们每一个人都必须开始反省自己的行为。因为,到了世界只剩下人类的那一天,生物多样性丧失将不再是诸多消亡物种的黄昏。     

生物机制在智能计算中的应用

揭示生物机制在智能计算中的应用。与生物学相关理论相结合 ,论述遗传算法、人工神经网络和DNA计算的生物机制 ,揭示生物学思想在其中的应用 ,说明现在各类学科交叉发展的趋势     

Kinetics of Growth and Nutrient Consumption in the Culture of Trichosanthes kirilowii Hairy Root

IntroductionThe hairy roots produced by Agrobacteriumrhizogenes infection are characterized by highgrowth rate and genetic stability. Thesegenetically transformed roots can produce similarhigher levels of secondary metabolites than intactplants[1] ,so hairy root cultures are a promisingmethod for producing valuable secondarymetabolites of many plants.Recently,two kindsof ribosome- inactivating proteins ( RIPs) [2 ] ,trichosanthin ( TCN ) [3 ] ,and TAP 2 9[4] wereidentified in Trichosanthes…     

生物毒素研究与应用展望

生物毒素是一类具有重要意义的生物源化学物质，已知化学结构的毒素可达数千种。生物毒素表现出生源、化学结构、作用机制等多方面的多样性。生物毒素研究已发展成为与多种学科交叉的新学科——毒素学，其发展对于生命化学、生源合成化学、化学生物学、化学生态学、医学、药物学、环境科学均有重要意义，对药物创新研究更具积极作用。许多生物毒素可作为临床药物或导向化合物，并可为药物分子设计提供有价值的新药效模型和结构构架，更能为发现药物新作用靶位发挥特殊作用。     

改变水分子簇的结构及诱发的生物效应研究

概述了水分子簇理论研究的基本状况，以及改变水分子团簇结构的4种方法，包括外加磁场、外加电场、激光辐射以及直接加热法；最后简要介绍了改变水分子团簇结构所诱发的生物效应。     

生物多样性保护与人类生存的关系

生物多样性是人类生存发展的基础，但社会的发展给生物多样性带来了严重的灾难，如何保护，如何培育，我们应高度重视并采取切实可行的措施。     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

气升式生物反应器内红霉素发酵的动力学分析

研究了气升式生物反应器内红霉素发酵的动力学行为，根据所归纳出的动力学方程，调整发酵过程中的补料策略，得出了比较优化的工艺和工程参数，如发酵液的C／N(碳源／氮源)比、氧摄取速度(OUR)等。根据此动力学模型，调整的优化发酵工艺使得菌体代谢周期缩短了13％，而红霉素发酵水平提高了10％。     

西北工业发展滞后的数量分析及调整对策

运用统计资料，从工业化基础、工业投入、工业内部结构、工业中非国有经济所占比重、工业的外向性等多个方面，分析了西北地区工业自改革开放以来整体下降的原因，说明工业增长滞后、工业效益滑坡是影响西北经济发展水平的重要因素。提出了在新的外部条件下，调整和优化西北地区工业结构的设想。