鹌鹑及与鸡的杂交后代3β-HSD和P-450c17基因的cDNA部分序列克隆

通过RT—PCR后回收测序的方法克隆了鸡、鹌鹑及其杂交后代目的基因部分eDNA序列,结果显示同源性的相似度分别为3β-HSD：鸡与鹌鹑98．06％;鸡与其杂交种96．12％;鹌鹑与其杂交种100％;鸡与NCBI已有报道的参照序列为95．15％;鹌鹑与杂交种和参照序列相比为98．06％。P-450c17：鸡与鹌鹑100％;鸡与其杂交种100％;鹌鹑与其杂交种100％;与参考鸡序列的同源性为99．29％。     

玉米黄色素的提取和分析

使用超临界CO2法从水解玉米蛋白粉中提取玉米黄色素.通过单因素实验和正交实验优化了超临界CO2萃取玉米黄色素的工艺条件,并通过HPLC检测提取物中玉米黄素和叶黄素的含量.结果表明:超临界CO2提取玉米黄色素的最佳条件为:萃取时间2 h,CO2流速35 kg/h,萃取压力30 MPa,萃取温度45℃,夹带剂15%无水乙醇,此时玉米黄色素产量为210.97 μg/g.     

Advances in the understanding of inter-subspecific hybrid sterility and wide-compatibility in rice

Hybrid sterility is a major form of postzygotic reproductive isolation and frequently occurs in hybrids between divergent populations, such as the indica and japonica subspecies of Asian cultivated rice （Oryza sativa L.）. It has been a major barrier for utilization of the strong heterosis expressed in hybrids between indica and japonica. A large number of loci for rice inter-subspecific hybrid sterility have been identified by genetic analysis. Cytological studies revealed that male and female gamete abortions and reduced affinity between the uniting gametes all occurred in indica-japonica hybrids, suggesting the complexity of the causes for inter-subspecific hybrid sterility. Two genes conditioning embryo-sac and pollen sterility respectively in indica-japonica hybrids have been cloned recently, providing opportunities for molecular characterization of the indica-japonica hybrid sterility and wide-compatibility. Future studies should aim at cloning more genes for indica-japonica hybrid sterility, characterizing the underlying molecular mechanism, and utilization of the findings for the development of inter-subspecific hybrids to increase rice productivity.     

Evolutionary analysis of allotetraploid hybrids of red crucian carp × common carp, based on ISSR, AFLP molecular markers and cloning of cyclins genes

The allotetraploid hybrids of red crucian carp x common carp are the first reported artificially cultured polyploid fish with bisexual fertility and stable inheritance in vertebrate. Using ISSR and AFLP markers and the cyclins genes, the genomes and cyclin gene sequence changes were analyzed between the allotetraploid hybrids and their parents. The results indicated that the allotetraploids inherited many genetic characteristics from their parents and the genetic characteristics were stable after 15 generations. However, the allotetraploids had a closer genetic relationship with their original female parents and represented a bias toward the maternal progenitor. DNA fingerprinting analysis showed that the allotetraploids had undergone sequences deletion from their original parents and that the deleted sequences were mostly from the male parent＇s genome. Some non-parental bands were found in the allotetraploid hybrids. Sequences analysis of the cyclin A1 and B1 genes showed nonsynonymous substitutions of single nucleotides in codons that were different from their original parents, leading to non-parental amino acid loci. We speculate that the non-additivity in the allotetraploids, compared with their progenitors, could be an adjustment to the genomic shock from heterozygosity and polyploidy, allowing maintenance of genetic stability.     

一个综合性生物化学实验的设计

根据生物化学课程实验的实际情况,提出以玉米须多糖的分离纯化及其性质测定为主题开设综合设计性实验。此实验的开出不仅可以提高学生在生物化学方面的综合实验技能,将理论与实践有机地结合起来,而且巩固了课堂所学的理论知识,同时还可以培养学生的科研和创新精神。     

玉米大垄垄上机械化行间覆膜栽培技术研究推广

玉米大垄垄上行间覆膜栽培技术是针对黑龙江省春旱、低温气候特点所采取的以地膜为载体,以机械化覆膜为手段,良种良法配套,农艺农机相结合的一种新栽培模式。因该栽培模式具有抗旱、增温、保墒、集雨,提高肥料利用率以及防终霜冻害的作用,所以采用大垄垄上行间覆膜栽培模式可达到增产、提质、增效的目的。     

玉米大豆超高产栽培技术总结

大豆玉米超高产栽培法是以应用优质高产耐密良种、采取大垄双行栽培、增加密度等为主要措施的增产技术。种植方式由原来传统的65公分或70公分垄作玉米,两垄变一垄,垄距为130公分,垄上种双行,大行距90公分,小行距40公分。玉米超高产栽培技术较一般种植技术亩产平均增加160—500公斤,同时降低玉米含水量4—5个百分点,提高了玉米的品质。     

通辽市特种玉米种植气候区划分析

根据玉米自身的特性和不同用途,将玉米分为高油型玉米、高淀粉型玉米、高赖氨酸型玉米和甜玉米四大类型．基于每种玉米品种对气候条件、土壤类型、地形特征等的不同需求,提出了特种玉米的区划指标,并利用GIS技术进行适宜种植区域划分．通辽市大部分农区适宜种植特种玉米,最适宜区、适宜区基本上均位于通辽南部和中部农业区．     

宋同明：中国高油玉米奠基人

宋同明,中国农业大学教授,博士生导师,玉米遗传育种专家,国内研究高油玉米并促进其产业发展的第一人。1981年至1983年在美国伊利诺大学进修,美国7位著名的玉米遗传育种专家,     

头大=聪明？玉米博士请回答

如果你哪天走在路上自自语：“今天会不会下雨啊？”从你身旁经过的老爷爷突然停下：“我认为不会！你看那些云……从气象学来看……根据科学常识来讲……所以下雨的几率很小。”咳咳,恭喜你!你见到了传说中的玉米博士！