家蚕非编码RNA Bm-152功能初探

Bm-152是前期研究中发现的一个在家蚕丝腺中高表达的非编码RNA.采用实时荧光定量PCR技术对Bm-152在家蚕5龄到预蛹期不同发育天数丝腺中的表达谱进行检测.同时,在个体中过表达Bm-152,检测Bm-152可能参与的信号通路.结果显示,Bm-152在家蚕5龄幼虫丝腺发育过程中无明显的变化规律,但在吐丝第1天表达量较高,随后表达量又下降.通过在血淋巴中注射2μg piggyBac[A3-EGFP-A3-Bm-152]过表达载体,48h后发现Bm-152在丝腺组织中无明显过表达,但在非丝腺组织中可实现明显过表达,且表达量上调1.66倍;蜕皮激素信号通路基因Usp在非丝腺组织中上调1.95倍,E75A在非丝腺组织中上调2.26倍,保幼激素信号通路基因Met在非丝腺组织中上调1.79倍.表明非编码RNA Bm-152可能通过蜕皮激素和保幼激素信号通路参与家蚕发育,该结果为进一步探索Bm-152的功能提供了方向.     

后现代语境中的《荒野小站》解析

20世纪后半叶,后现代主义形成一股强大的文化思潮,深刻影响了当代西方作家的文学创作。加拿大短篇小说家艾丽丝·门罗,尽管被很多研究者认为是一个现实主义作家,但其小说中常常出现的含混、不确定显然来自后现代语境。《荒野小站》就是这样一个例子。这篇小说在叙事手法上出现的文类混杂、碎片化和多重不可靠叙述所带来的不确定性,以及对女人的疯狂所做的思考,都较为集中地体现了后现代文学的基本特征。     

管螺纹三轴滚丝加工工艺

本文通过对管路管螺纹三轴滚丝加工工艺的分析,介绍管螺纹滚丝与普通切削管螺纹的不同,及其优势、特点,并介绍了滚丝的工艺改进,以及目前还存在的问题。     

The effects of the non-breaking surface wave-induced vertical mixing on the forecast of tropical cyclone tracks

The Weather Research and Forecasting model(WRF)is configured for the region of(15°–41°N,105°–135°E),which covers the same area with the MASNUM(Key Lab.Marine ScienceNumerical Modeling,State Oceanic Administration)wave-tide-circulation coupled operational forecast system.Three numerical experiments are implemented to investigate the effects of the real-time forecasted sea surface temperature(SST)and the nonbreaking wave-induced vertical mixing(Bv)on the track forecast of all 33 tropical cyclones(TC)in the model domain area during 2008 and 2011.The first experiment employs NCEP FNL(NCEP final analysis)SST as WRF’s bottom condition as the Control run,which is also the default setup of WRF.The second and third experiments use real-time forecasted SST from the MASNUM forecast system with and without Bv,respectively.The forecasted track results are compared with Japan Meteorological Agency’s best track data.For 24-h forecast,the averaged TC position error of Experiment with Bv is reduced by 9%compared to the Control experiment,while the forecasted track error of Experiment without Bv is reduced by only2%compared to the Control experiment.For the 48-h forecast,the averaged track errors are reduced by 10%and6%with Bv and without Bv compared to the Control experiment,respectively.These results suggest that the real-time forecasted SST can improve the performance of WRF in forecasting TC track,and the Bv plays an important role in reducing the forecast error of TC track.Comparatively,Bv can improve more on the track of stronger TC.     

洋流,奏响海洋生物的欢歌

正洋流,一种大规模的海水运动,周而复始,永不停歇。从单胞藻到南极企鹅,从红树林到珊瑚礁,依赖着海洋而生的动植物们,是如何在各股洋流的影响下,一代代地繁衍昌盛,共同演奏出这曲欢腾高亢的生命之歌的呢?     

基于RNA-Seq技术的毕赤酵母基因组重注释及新型强启动子的发现与序列分析

使用RNA-Seq技术,对毕赤酵母进行了转录组测序。基于测序结果,对现有毕赤酵母基因组进行了重注释,修正了基因组序列中的错误位点861处,发现了新转录本249个及可变剪切现象83个,并更正了553个转录本的错误注释。经过表达谱分析,在毕赤酵母中发现了2个新型强启动子,分别命名为P437和P1431,其驱动的转录本的最高转录水平分别为AOX1基因的1.78倍和3.40倍。序列分析显示,P437和P1431序列中存在着多个酵母转录因子的结合位点。     

Simulation of sea surface temperature changes in the Middle Pliocene warm period and comparison with reconstructions

The Middle Pliocene (ca 3.12–2.97 Ma) is a recent warm period in the Earth’s history. In many respects, the warmth of the Middle Pliocene is similar to the probable warm situation of the late 21st century predicted by climate models. Understanding the Middle Pliocene climate is important in predicting the future climate with global warming. Here, we used the latest reconstructions for the Middle Pliocene—Pliocene Research Interpretation and Synoptic Mapping (PRISM) version 3—to simulate the Middle Pliocene ...     

多丝正比计数器的优化设计

由于低水平放射性测量中样品计数率非常低,使用多丝正比计数器进行α放射性测量时,本底主要由探测器内壁材料中的α放射性成分贡献.考虑到测量系统本身的放射性干扰一直是制约测量能力的重要因素,降低测量系统本底是α辐射测量的重点.采取在传统多丝正比室添加屏蔽丝的方法,并通过Garfield程序模拟正比室在不同参数设置下电子漂移过程得到正比室各项参数的最优解.模拟结果表明,优化后的正比室可以对不同入射方向的α粒子进行筛选,有效抑制探测器本底,从而得到最优的正比室结构设计参数.     

Different process parameters on the effects of tobacco quality

通过正交试验探讨了工艺参数与烟丝填充值、整丝率及烟丝经过卷烟机后烟丝填充值、整丝率降低之间的关系,寻找参数间的最佳组合.结果表明:①在试验范围内,各因素对烟丝整丝率降低影响较显著；薄板烘丝机滚筒转速对烟丝填充值影响较显著,对烟丝填充值降低达到显著性影响.各因素对烟丝整丝率影响不显著；松散回潮出口含水率、叶丝增温增湿机(HT)开度对烟丝填充值、烟丝填充值降低影响不显著.②试验因素最佳组合为松散回潮出料烟叶含水率19.5±1.0％、叶丝增温增湿机HT开度74％、薄板烘丝机滚筒转速13 r/min.