Transformation of the salt-tolerant gene of<Emphasis Type="Italic">Avicennia marina</Emphasis> into tobacco plants and cultivation of salt-tolerant lines

Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions.     

AC2 and AC4 proteins of <Emphasis Type="Italic">Tomato yellow leaf curl China virus</Emphasis> and <Emphasis Type="Italic">Tobacco curly shoot virus</Emphasis> mediate suppression of RNA silencing

Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) alone could systemically infect host plants such as Nicotiana benthamiana without symptoms. In contrast, Tobacco curly shoot virus Y35 isolate (TbCSV-Y35) alone induces leaf curl symptoms in N. benthamiana. When inoculated into transgenic N. benthamiana plants expressing GFP gene (line 16c), TYLCCNV-Y10 neither reverses the established GFP silencing nor blocks the onset of GFP silencing. In contrast, TbCSV-Y35 can partially reverse the established GFP silencing and block the onset of GFP silencing in new leaves. In the patch co-infiltration assays, the AC2 and AC4 proteins of TYLCCNV-Y10 and TbCSV-Y35 could suppress local GFP silencing and delay systemic GFP silencing, suggesting that they are suppressors of RNA silencing. Comparison of the accumulation levels of GFP mRNA in the co-infiltration patches showed that Y10 AC2 and Y35 AC2 proteins had similar efficiency for suppression of RNA silencing. However, Y35 AC4 protein functioned as a stronger suppressor of RNA silencing than Y10 AC4 protein. There-fore, the pathogenicity difference between TbCSV-Y35 and TYLCCNV-Y10 may be related to the functional difference in their AC4 proteins.     

具有AP2结构域的转录因子家族的研究进展

具有AP2结构域的转录因子是一种被认为只存在于高等植物中的转录因子,但根据最新的研究发现在一些低等的生物中同样存在该转录因子。这是一种基因的入侵,具有AP2结构域的转录因子在高等植物中存在有几百个。本文综述了具有AP2结构域的转录因子的研究进展,包括三个方面:1、具有AP2结构域的转录因子是一个从低等生物到高等生物进化上的入侵基因;2、具有AP2结构域的转录因子的分子结构及功能;3、在高等植物中该转录因子的研究进展情况。此外还展望了一下这个基因家族的研究前景。     

植物试管苗移栽过程中气孔开度的变化研究

用分步炼苗法，对烟草，草梅栝楼的试管苗进行了开口光锐、沙培和室外营个步骤的炼苗；     

矿质肥料增效剂在烟草生产中应用初报

通过开展施用矿质肥料增效剂对烟叶产质量及品质影响的初步研究,结果表明,在常规施肥的前提下,增施矿质肥料增效剂,能够促进烟株生长发育,提高烟叶的经济效益,改善烟叶品质。     

一种快速检测植物病毒RNA蓄积量的竞争PCR方法

用Oligo6.6软件在pUC18上设计1对比目的基因多100～200 bp的内参引物,摸索了目的基因和内参共同扩增的竞争PCR反应条件,测定了嘧肽霉素处理对烟草组织中烟草花叶病毒RNA相对含量的影响,同时用间接ELISA方法验证了该方法.试验结果表明:竞争PCR的方法能够快速、相对准确的测定植物组织中病毒RNA蓄积量的变化情况.     

远离烟草，远离肺癌

本文是国际肺癌新闻奖最佳消费者奖的获奖报道,该评选是由一个享有威望的独立小组评出的,这个小组由来自于宣传、临床和媒体界的多学科的重要意见领袖(opinion leader)以及一位肺癌患者组成。这些奖项旨在通过表彰三个不同种类的新闻界杰出表现来鼓励对肺癌进行准确而富有影响力的报道。这三个奖项种类为:最佳消费者奖(Best Consumer Award)、最佳医学奖(Best Medical Award)和最佳广播电视报道奖(Best Broadcast Report)。     

多效烟草抑芽剂MH的合成研究

研究了以水合肼，顺酐，硫酸为原料合成ＭＨ的方法，讨论了原料配比，反应温度等因素对产物收率的影响，由ＩＲ谱，＾１３Ｃ－ＮＭＲ〈Ｈ－ＮＭＲ确证了产物的结构。     

内蒙古烟草小史：兼论民国前期内蒙古的烟税及有关机构

明朝末年，入朝鲜作战的东蒙军队将吸旱烟的嗜好带返本籍，而后，旱烟和鼻烟的吸食便从东北及内蒙古之南的汉地传入内蒙古，同治年间，山西商人开始在内蒙古开烟庄，销售烟草。光绪年间，内蒙古嗜鼻烟的人增多，后形成了交换玛瑙鼻烟壶以表示情谊的习俗。民国年间内蒙古有了完善的烟税规则及征税机构。     

以烟草企业为例论我国行政垄断公司的治理

文章以烟草企业为切入点,讨论了我国行政垄断企业在公司治理方面存在的弊端,并且给出完善我国行政垄断企业公司治理结构的建议。