直立设备受风诱导振动的计算

文中介绍了对于直立设备受风后的诱导振动产生的原因，工程计算及防振措施。     

中华芦荟愈伤组织的诱导

以中华芦荟幼苗茎段为外植体,以MS为基本培养基,通过添加不同浓度的生长素、细胞分裂素或两者组合,以及添加Vc或活性炭(共350种组合),研究中华芦荟愈伤组织的诱导及生长情况.结果表明, 在单独使用不同种类的生长素时,不同浓度的生长素诱导的愈伤组织频率差异明显,且愈伤组织的生长状态也各异;单独使用细胞分裂素时,愈伤组织的诱导率极低.当组合不同浓度和种类的生长素和细胞分裂素时,愈伤组织诱导频率差异显著;同时发现在减轻愈伤组织褐化的效果上,Vc较活性炭好.通过对愈伤组织诱导结果的比较,MS 2.0 mg/L 6-BA 0.5 mg/L 2,4-D 10 mg/L Vc为中华芦荟愈伤组织诱导的较好培养基,愈伤组织诱导率达到91.4%,且愈伤组织生长较旺盛.     

基于遗传算法多目标非重叠路径诱导算法

依据遗传算法基本原理,提出一种多目标路径诱导算法. 染色体编码采用可变长度节点序列方法表示,以减少染色体编码长度. 设计了相应的多目标适应度函数. 该算法在给定多个目标约束条件下,能够解出多个近优路径. 实验结果证明,该算法能有效解决多目标非重叠路径选择问题,和目前已有其它方法相比,路径相似度更小.     

Effect of cold spray particle conditions and optimal standoff distance on impact velocity

冷喷涂中,粒子撞击基板时的速度对涂层形成起到至关重要的作用,而适当的喷涂距离可以使喷涂粒子获得较高的速度.利用商用CFD软件Fluent模拟了喷嘴外超音速流场的分布情况,分析了粒子自身条件对冲击速度的影响,同时提出了确定粒子与基板最佳距离的方法.分析发现,小尺寸、低密度以及不规则形状的粒子容易受到喷嘴外产生的膨胀波、斜激波和基板前的弓形激波影响.随着能量的耗散或当粒子穿过弓形激波时,这些粒子的速度明显下降.另外,在基板与喷嘴之间存在一个最佳距离,它的确定需要两个必要条件：一是基板处的气流必须保持较低的速度,从而使弓形激波的强度相对较弱;二是粒子须经膨胀波加速2次,即弓形激波前具有2个膨胀波,以确保气体可充分利用膨胀波的加速.     

Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation

Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes, wild and domesticated. Each form differs conspicuously in root phenotype, but can be converted from one type to another by habitat. Here we show that the habitat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′ sites detected by the methylation-sensitive polymorphism (MSAP) marker. The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form. Interestingly, artificially transplanted ginseng plants recreated in both directions the methylation levels (at least in CHG) of their natural counterparts. The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon. Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng.     

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis （Guenee）

The roles of signaling pathways in the production of trypsin proteinase inhibitors (TrypPIs) in rice infested by the leaf folder (LF) Cnaphalocrocis medinalis were studied. Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering, booting and flowering stages but decreased TrypPI levels at the ripening stage; TrypPI levels in rice stems did not increase at any developmental stage. Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems; it also enhanced salicylic acid (SA) levels in leaves and stems, and the ethylene level released from plants. However, LF infestation did not increase JA concentrations. Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage, whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone, suggesting an antagonistic effect of SA and ethylene on TrypPIs induction. The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF; moreover, the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs.     

油茶花药离体培养影响因子研究

以攸县油茶花药为外植体,研究了不同花粉发育时期、低温预处理时间及添加物对愈伤组织诱导的影响,同时分析了不同激素种类和比例对愈伤组织继代增殖的影响。结果表明:花粉四分体时期为油茶花药愈伤组织诱导的最佳时期;花药的低温预处理时间应在10 d以内;培养基添加物以AgNO3效果最优,诱导率最高为8102 ％。继代增殖培养中6-BA与NAA配比有着较好的效果,其中最适增殖培养基配方为:MS+6-BA（200 mg／L)+NAA(005 mg／L)。     

低温-秋水仙素诱导甜瓜多倍体研究

以刚展开子叶的甜瓜植株为材料,采用低温-秋水仙素处理,植株全部成活,通过检测气孔-叶绿体的变化情况,选出4株定植于营养箱内,采收全部植株所结果实,保留种子并测量.采收期过后,用嫩芽叶尖为材料,对3株甜瓜进行染色体计数检测,检出1个四倍体.结果表明:低温-秋水仙素处理可以有效降低秋水仙素的毒害作用,提高植株成活率.植株后期,有由四倍体回复到二倍体的现象,说明在植株生长后期倍性稳定性有所改变.     

酿酒酵母表面展示脂肪酶LipB52的酶学性质

酿酒酵母表面展示脂肪酶重组菌株EBY100-pLHJ026在含半乳糖(20 g/L)的YNB-CAA培养基中进行诱导表达,脂肪酶在诱导48 h时酶活达到最高。以不同碳链长度的对硝基苯酚酯为底物对全细胞酶活进行性质检测,结果表明:对硝基苯酚癸酸酯(C10)为最适反应底物;全细胞脂肪酶在pH 8.0时的最适反应温度为37℃,在60℃保温2 h酶活保持90%,在60℃保温3 h酶活保持55%,表现出较好的热稳定性。在等体积二甲基亚砜中处理3 h后酶活保持28.4%。     

路径诱导系统中综合地图匹配算法的研究

地图匹配算法对于车辆路径诱导和实时轨迹回放具有重要的应用,为了提供连续、精确和可靠的车辆行驶路段位置,利用定位传感器输出车辆运行位置信息,结合高精度空间道路网络数据,提出新的综合地图匹配算法.该算法不受地理环境的约束,能有效地修正传感器的定位误差,提高车辆定位精度.实际道路测试证明,该算法比现有地图算法更高准确度和实时性,尤其是在十字路口有更好匹配效果.