稻作最优理论与方法研究

以作物群体产量密度理论模型及有关产量性状的稻作生物学机制为理论基础,结合稻作控制技术的发展,以尽可能提高群体产量为目的,提出了依分段目标逐级优化而实现稻作整体最优的稻作优化控制设计。该理论与方法体系的建立,奠定了现代稻作诊断与设计的基础。     

两系法杂交稻母本变异的原因及对策探讨

两系法杂交水稻现有母本－光敏雄性核不育系变异不仅比常规品种快,甚至比“三系稻”的母本还要快,阻碍该项技术应有潜力的发挥。介绍“两系稻”现有母本的遗传变异性过强的现象。分析了其成因,并对进一步纯化其光敏性与温敏性的育种策略做了初步探讨。     

模拟污水中氮,磷对水稻劝苗过氧化氢酶和乙醇酸氧化酶…

以水稻为材料，研究了不同深度的模拟污水中Ｎ、Ｐ对幼苗过氧化氢酶（Ｃａｔａｌａｓｅ，ＣＡＴ，ＥＣ１．１１．１．６）、乙醇酸氧化酶（Ｇｌｙｃｏｌａｔｅｏｘｉｄａｓｅ，ＧＯ，ＥＣ１．３．３．１）、过氧化和物酶（Ｐｅｒｏｘｉｄａｓｅ，ＰＯＤ，ＥＣ１．１１．１．７）和叶绿素含量的影响。结果表明：低浓度Ｎ、Ｐ浓度分别超过８．５６ｍｍｏｌ．Ｌ＾－１和０－０．２ｍｍｏｌ．Ｌ＾－１范围）可诱导ＣＡＴ活性增大，而后下     

水稻幼苗对几种无机元素离子吸收的研究

水稻(品种:光辉)幼苗吸收k~+、NH_4~+、NO_3~-、H_2PO_4~-与这些离子在吸收液中的浓度关系是非常简单的。K~+、H_2PO_4~-、No_3~-的吸收与它在吸收液中的浓度具有显著的正相关。NH_4~+的吸收不受吸收液中的NH_4~+和其它离子的显著影响。从整体来看,离子吸收又是平衡的。     

Inducible expression pattern of rice Bowman-Birk inhibitor gene<Emphasis Type="Italic">OsWIP1-2</Emphasis> and its protease inhibitory activity

The WIP1-2 gene was cloned from rice. It be-longs to the Bowman-Birk inhibitor gene family. Northernblot showed that expression of this gene was induced bywounding and jasmonic acid (JA). It indicates that the OsWIPI gene plays an important role in the rice defense sys-tem. The OsWIP1-2 was cloned into pET28a and expressed inE. coli. Its expressed product was purified in the form offusion protein and tested for the inhibitory activities againsttrypsin and chymotrypsin. It was found that the fusion pro-tein could inhibit chymotrypsin, but not trypsin. It was alsofound that the His tag at its C-terminal affected its inhibitoryactivity significantly. The fusion protein with a naturalC-terminal had the inhibitory activity, while no inhibitoryactivity was detected in the fusion protein with a (His)6-tag atits C-terminal. This implies that extra amino acid residues atthe C-terminal of OsWIP1-2 may interfere with its correctfolding. The inhibitory assay indicated that the members ofrice Bowman-Birk inhibitor gene family probably differenti-ated both in their structure and function.     

A CHASE domain containing protein kinase OsCRL4, represents a new AtCRE1-like gene family in rice

AtCRE1 is known to be a cytokinin receptor inArabidopsis. The AtCRE1 protein contains CHASE domain at the N-terminal part, followed by a transmitter (histidine kinase) domain and two receiver domains. The N-terminal CHASE domain of AtCRE1 contains putative recognition sites for cytokinin. Five CHASE domains containing proteins were found in rice, OsCRLla, OsCRLlb, OsCRL2, OsCRL3, and OsCRL4. OsCRL1a, OsCRL1b, OsCRL2 and OsCRL3 contain the four domains existing in CRE1, whereas OsCRL4 only contains the CHASE domain and a putative Ser/Thr protein kinase domain The authors cloned the encoding gene OsCRL4 and found that it represents a new member of the cytokinin receptor protein in rice.