Transformation of the salt-tolerant gene of<Emphasis Type="Italic">Avicennia marina</Emphasis> into tobacco plants and cultivation of salt-tolerant lines

Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions.     

Mathematical modeling of salt-gradient ion-exchange simulated moving bed chromatography for protein separations

The salt-gradient operation mode used in ion-exchange simulated moving bed chromatography (SMBC) can improve the efficiency of protein separations. A detailed model that takes into account any kind of adsorption/ion-exchange equilibrium, salt gradient, size exclusion, mass transfer resistance, and port periodic switching mechanism, was developed to simulate the complex dynamics. The model predictions were verified by the experimental data on upward and downward gradients for protein separations reported in the literature. All design and operating parameters (number, configuration, length and diameter of columns, particle size, switching period, flow rates of feed, raffinate, desorbent and extract, protein concentrations in feed, different salt concentrations in desorbent and feed) can be chosen correctly by numerical simulation. This model can facilitate the design, operation, optimization, control and scale-up of salt-gradient ion-exchange SMBC for protein separations.     

融盐包裹法合成球形正极材料LiNi_(0.7)Co_(0.2)Al_(0.1)O_2(英文)

以球形Ni(OH)2为核心原料,Al(NO3)3.9H2O、Co(NO3)2.6H2O和LiNO3为包裹原料,采用融盐包裹法在空气中煅烧合成了单相固溶体LiNi0.7Co0.2Al0.1O2。用XRD研究了合成产物的物相和结构,用SEM研究了合成产物的形貌,用电池性能测试仪研究了合成产物的电化学性能。实验结果表明,合成产物具有α-NaFeO2型层状有序结构、球状形貌和良好的电化学性能。     

盐分胁迫对绿豆幼苗若干生理生化指标的影响

实验采用水培的绿豆幼苗,模拟水分的积盐状况,用不同浓度的NaCl溶液作为渗透胁迫剂,分别用2%,4%,6%NaCl溶液对绿豆幼苗进行处理,观察其生长状况,测定其平均植株高度,脯氨酸含量。结果显示,随着盐分浓度的升高,绿豆幼苗伤害加重,生长状况不断恶化,植株平均株高降低,叶片及根中的脯氨酸含量均呈递增状况。     

一株耐盐芽孢杆菌的分离与鉴定

从校园草坪土壤中分离得到一株能够在20%NaCl的高盐条件下生长的耐盐细菌,经过形态观察、生理特征分析及16SrDNA序列分析,鉴定为一株芽孢杆菌(Bacillussp.),并在GenBank中完成该菌株16SrDNA序列的注册,登录号为EU167738.     

几种不同盐生植物叶的比较解剖研究

对宁夏地区5种盐生植物叶的形态结构特征进行了比较解剖学方面的研究．结果表明,5种盐生植物在叶的外部形态和内部显微结构方面存在着一定的差异．     

盐胁迫下金银花叶片叶绿素含量的测定与分析

金银花,属忍冬科(Caprifoliaceae)忍冬属(Lonicera)多年生半常绿藤本植物.全世界约有200种,在我国也分布甚广.其药用价值及经济价值被人们广泛认可,但耐盐性方面却涉及甚少[1].据了解,在山东禹城盐分含量高达0.5%的盐碱地中种植忍冬,不仅可以正常生长,而且产量也较高.本实验对不同浓度的盐胁迫下金银花叶绿素的变化进行了初步测定和分析,旨在为金银花的耐盐性研究提供些依据.     

盐度和营养限制对盐生隐杆藻生长和胞外多糖产率的影响

盐生隐杆藻在整个生长期内都不断地释放胞外多糖,而且进入静止期后释放量增大,盐生隐杆藻胞外多糖的不断释放,使培养液相对粘度不断增加．０．７５ｍｏｌ／Ｌ ＮａＣｌ盐度是盐生隐杆藻生长和释放胞外多糖的最适盐度,更高或更低的盐度不利于生长和胞外多糖的释放,营养限制对盐生隐杆藻生长的影响从大到小为：ＮＯ＾－３〉Ｍｇ＾２＋〉Ｋ＾＋〉Ｃａ＾２＋〉ＳＯ＾２－４〉ＰＯ＾３－４。从每个藻细胞外多糖的产率看,Ｍｇ＾２＋     

盐胁迫下小麦新品系89122的抗氧化 酶类活性变化的研究

利用受粉后的花粉管通道将高梁DNA导入普通小麦陇春13号,在其后代中出现了广泛变异,从中选育出已稳定遗传的丰产、抗逆性强的新品系89122．经大田和盐池鉴定,89122的耐盐性明显高于陇春13号．用NaCl(0, 0.1, 0.15, 0.2, 0.25　mol/L)处理89122和陇春13号幼苗7　d,测定盐胁迫后其幼苗中抗氧化酶类活性的变化．结果发现 ,转基因小麦89122较其受体陇春13号能维持较高的SOD,CAT及POD活性．并且以上几种酶之间能保持很好的平衡和协调表达．表明具有较高的清除活性氧的酶活力与小麦耐盐性紧密相关,可将其作为转基因小麦耐盐筛选的指标之一．     

植物谷胱甘肽转移酶和盐胁迫

谷胱甘肽转移酶 (GSTs)的种类不一 ,在植物异生代谢和内生代谢中都表现了一定的功能 ,通过转基因技术使之在植物体中过量表达显示它在植物耐盐过程中也起到了一定的作用 .