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131.
132.
嗜盐细菌冷冻干燥保护剂的正交法优化 总被引:2,自引:1,他引:2
报道了利用正交试验设计的原理和方法,筛选冷冻干燥保藏盐生盐杆菌菌株R1的保护剂组成与佳配比的结果,直接分析和方差分析的结果表明,在由NaCl,水解乳蛋白、海藻糖和蔗糖4种成份组成的保护剂系统中,海藻糖对菌株R1冷冻干燥存活率影响最大,为主要因素;其次为蔗糖和NaCl;水解乳蛋白影响最小,为次要因素,保护剂最优的水平组合为NaCl 15%、水解乳蛋白4%、海藻糖6%、蔗糖18%。采用正交法从传统“简 相似文献
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135.
小麦抗盐突变体质膜K+通道K+, Na+通透性的改变 总被引:3,自引:0,他引:3
突变体K^+通道在100mmol/L KCl和NaCl溶液中对Na^+、K^+离子的通透性均显著低子野生裂。突变体K^+通道在KCl和NaCl溶液中开放频率均显著低于野生型,突变体K^+通道Na^+、K^+通透性的降低是与其开放频率下降分不开的,突变体和野生型K^+通道的单通道电导性基本一致,两者的差异主要表现在开放频率上。两者K^+通道对K^+/Na^+通透比所表现出的选择性却无明显差异,分别为 相似文献
136.
盐法分离低核酸酵母蛋白的机理研究 总被引:1,自引:0,他引:1
本文用荧光光谱法研究了不同盐对酵母蛋白质构象的影响,结果表明,盐是降低酵母蛋白分离物的核酸含量的原因之一。通过对盐除核酸过程进行热力学分折,对比离子对水结构和核蛋白稳定性的影响,否定了Damodaran提出的疏水作用机理,证明在维持核蛋白稳定的次级键中,氢键的作用是最主要的;核蛋白的解离并非盐通过改变溶剂的结构而间接作用于核蛋白所致,而应归因于盐与核蛋白的直接作用,特别是盐与核蛋白分子主链的作用。 相似文献
137.
本文采用复分解法,首次合成出Ln_2As_2W(18)O_(62)xH_2O(Ln:La,Ce,Nd,Sm,Gd.)系列杂多酸稀土盐,紫外吸收光谱、红外吸收光谱、x—射线粉末衍射分析都证明所合成产物具有Dawson结构,用差热——热重分析及溶液分光光度等手段,研究了合成产物的热性质和水溶液的酸碱稳定性。 相似文献
138.
WUDexing WANXiuquan BAOXianwen MULin LANJian 《科学通报(英文版)》2004,49(4):363-368
TheBohaiSea(37°07′—41°N,117°35′—121°10′E)isashallowsemi-enclosedsea.SincetheBohaiSeaistheonlyinnerseaofChinaandalsoanimportantstra-tegicsea,itisoneofthemostinvestigatedseasinChina.Alargenumberofstudiesonthetideandthetidalcurrentaswellasnumericalsimulationofcirculationhavebeencarriedout,enhancingourunderstandingoftheBohaiSea.Long-termvariationsoftheseasurfacesalinity(SSS)andseasurfacetemperature(SST)oftheBohaiSeawereanalyzed.Linetal.[1]foundthatthepositivetrendsoftheannualmeanS… 相似文献
139.
QISuwei YANGPingfang JINGYuxiang SHENShihua YANGSusheng 《科学通报(英文版)》2004,49(17):1828-1833
Sinorhizobium fredii RT19, a strain of freeliving bacteria, was subjected to salt shock and its protein expression profiles were analyzed by differential display proteome approaches. The results of separation by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) showed that the number of resolved proteins was 481, 465 and 424, corresponding to salt-free control, 5 and 50 min 1 mol/L salt treatment, respectively. Among the resolved proteins, 82 in total had altered expression in response to salt-shock stress. 26 out of the 82 proteins were induced and 23 were completely inhibited, while 12 were up-regulated and 21 down-regulated in response to salt shock. In addition, the appearance of differentially displayed proteins responding to different salt shock periods is also reported. The identity of the 26 induced proteins was revealed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) followed by database searching. Among them, 20 were assigned to proteins with known functions. Their roles in response to salt shock stress are discussed. 相似文献
140.
ZHOUHantao LINQingtong PANWen GAOYuanyuan CHENPan CHENXu LIUBo 《科学通报(英文版)》2004,49(5):456-461
Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions. 相似文献