如何提取用于法医物证检验的生物检材

本文介绍了法医物证检验中通常提取的四种生物检材，并对每种生物检材的提取注意事项作了具体分析。     

The Biocompatibility of Wool Keratin

Keratin is the major structural fibrous protein providing outer covering such as hair, wool, feathers, etc. When being used as a kind of biomaterials, the biocompatibility of wool keratin is one of the most critical questions. By now, there has not been systemic study on the biocompatibiiity of keratin. Therefore, in this article we used the procedures of skin irritation, haemolysis and subcutaneous implantation according to ISO 10993 to study it. Moreover, the Fourier transform-infrared (FTIR) spectroscopy was utilized to analyse the impurity and structure modification of wool keratin film. The part of the animal tests showed that the wool keratin films prepared by authors were biocompatible. But the residual of sodium dodecyi sulfate (SDS) affected the results of other tests. Consequently, the wool keratin membrane is one kind of favourable and promising biomaterial for biomedical and histological utilization. The residual SDS used as an agent should be eliminated from the keratin solution or membrane completely if for biological usage. In conclusion, wool keratin, as a kind of natural protein, prospectively could be applied in biomedical materials and scaffolds of tissue engineering.     

羊毛角蛋白再生技术研究进展

根据废旧羊毛资源现状，羊毛角蛋白再生技术的研究具有良好科学意义和显著应用前景。本文介绍了羊毛角蛋白的结构与化学性质，角蛋白溶液的制备方法、难点问题以及纤维化再生利用的研究现状。     

孕妇头发中七种元素的回归分析

用计算机对不同妊娠期孕妇头发中Ｆｅ、Ｚｎ、Ｃａ、Ｃｕ、Ｍｇ、Ｍｎ和Ｓｅ对胎儿体重、胎儿股骨长、胎儿双顶径生长的影响进行回归分析。结果表明，７种元素与胎儿的和平共处发育有着密切的联系。不同妊娠期，各种元素影响的程度不同，ｐｂ在整个妊娠期无明显变化。     

细胞角蛋白免疫组化染色在乳癌腋淋巴结转移检测中的价值

13例乳癌共取腋淋巴蛄189枚,每例平均14.69±4.42枚。每枚淋巴结分别作连续6张切片H、E染色和1张切片细胞角蛋白免疫组化(CKIHC)染色,光镜下现察。结果H、E染色检查13例中7例淋巴姑转移,转移率53.85％,淋巴结转移数目19枚,转移度10.05％;CKIHC染色检查淋巴结转移8例,转移率61.54％,淋巴结转移数目21枚,转移度11.11％。虽然二种方法检查腋淋巴结转移率和转移度结果无显著性差异(P>0.05),但不仅HE染色发现有转移的淋巴结CKIHC染色皆为阳性,而且HE染色未能发现的微小转移CKIHC染色亦可检出。即CKIHC染色使腋淋巴结癌转移的检出增加。因而可以认为1张切片CKIHC染色可替代连续6张切片HE染色。     

毛发中滥用药物及其代谢产物的检验

本文对毛发中滥用药物检验的重要性及历史和检验技术进行了综述,并对各种检验技术的利弊进行了分析.     

在井岗霉素生产中使用角蛋白水解物作氨基氨源的研究

畜、禽毛羽经水解加工后形成的角蛋白水解物，不仅是一种氨基酸含量丰富的畜、禽动物饲料蛋白营养源，而且可作为微生物发酵时的氨基氮源。为降低成本，扩大井岗霉素的生产量，我们在摇床试验和１７吨位罐的生产性试验基础上，证实每吨发酵基质料中用１．３％量的角蛋白水解物，代替传统的酵母粉加蚕蛹粉作氨基氮源时，井岗霉素的生成量可从原有基础上提高７％（Ｐ＜０．０５），而投料成本可降低近２７％的良好效果。根据角蛋白水解物的氨基酸组成分析，对利用吸水链霉菌发酵生产井岗霉素中所应具备的氨基氮源，提出了分析和探讨．     

毛发水解制取胱氨酸的工艺改进

毛发中胱氨酸含量约为14%,而且工业化大生产中不少厂家的精品收率不到7%.究其原因,除毛发质量与水解效果外,主要在于因工艺设计与操作方法不当,致使一二