有界域上具有离散核的Cauchy公式和-方程

设D是Cn空间中具有C(1)边界 D的有界域,本文利用D上一个局部有限的可数强拟凸开复盖,定义了D上一个新的局部全纯的σ点有限的单位分解,建立了D上一个更一般的具有离散局部全纯核的Cauchy积分公式并获得D上 方程的具有离散核的解的积分表示.     

Genetic Diversity in Populations of Sepiella maindroni Using 16S rRNA Gene Sequence Analysis

Part of the 16S rRNA gene is amplified with PCR and sequenced for 5 populations of common Chinese cuttlefish Sepiella maindroni:three from the South China Sea,one from East China Sea and one from Japan.The result shows that a total of 5 nucleotide positions are found to have gaps or insertions of base pairs among these individuals,and 13 positions are examined to be variable in all the sequences,which range from 494 to 509 base pairs.All of the individuals are grouped into 7 haplotypes (h1-h7).No marked genetic difference is osberved among those populations.All of the individuals from Nagasaki belong to h1 and the h3 haplotype is found only in the coastal waters of China.A→←G transition in Nucleotide 255 is suggested to be taken as a kind of genetic marker to identify the populations distributed in East-South China Sea and the Nagasaki waters of Japan.     

基因疫苗及影响其免疫效果的因素

基因疫苗被视为“第3代疫苗“,是目前疫苗研究领域的热点.本文回顾了基因疫苗的诞生,阐述了其优越性,重点讨论了增强其免疫效应的策略包括合理的载体设计、细胞因子及共刺激因子与抗原的共表达及适宜的接种途径等.     

Recent progress in polymer-based gene delivery vectors

The gene delivery system is one of the three components of a gene medicine, which is the bottle neck of current gene therapy. Nonviral vectors offer advantages over the viral system of safety, ease of manufacturing, etc. As important nonviral vectors, polymer gene delivery systems have gained increasing attention and have begun to show increasing promising. In this review, the fundamental and recent progress of polymer-based gene delivery vectors is reviewed.     

N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Temperature variations on the Tibetan Plateau over the last two millennia

The paleoclimate data recovered from ice cores,tree rings and lake sediments indicate regional features of cfimatic change on the Tibeta n Plateau (TP) during the last 2000 years. The composite temperature reconstructions in-dicate that several main climatic episodes, such as the “LittleIce Age“ between 1400 and 1900, the “Medieval Warm Pe-riod“ in 1150-1400, a less warm period in 800-1100, and an earlier cold period between the 3rd and 5th centuries,occurred in the TP. In addition, temperature varied from region to region. The period from AD 800 to 1100, which waswarm in northeastern TP, was contemporaneous with cool-ing in the western and southern TP. The southern TP ex-perienced warming between 1150 and 1400. For western TP,the δ^18O records of the Guliya ice core indicate that the pe-Hod 1250-1500 witnessed a clear warming. Large-scaletrends in the temperature history from northeastern TP aremore similar to those in eastern China than are the trendsfrom the Guliya ice cap far to the west and southern TP. The most prominent similarities between the temperature varia-tions of the TP and eastern China are such cold phases as 1100-1150, 1500-1550, 1650-1700 and 1800-1850, andthe latter three cold events match with three widespreadg lacial advances which occurred on the TP during the Little Ice A2e.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

80位并行3—DCT硬件核解决方案

提出一种使用三维离散余弦变换（3D－DCT）对运动图像进行压缩解压的新算法。在此算法的基础上构造了一个80位3D－DCT硬件核，通过并行连乘连加运算加快计算速度、三维转置存储体消除矩阵转置耗时、中间寄存器消除中间计算结果的存取时间等综合手段，达到高速处理目的。FPGA实现的时序模拟表明，采用这种算法可以对现有制式的电视图像进行实时压解处理。     

表达千穗谷Ah—AMP基因的转基因烟草抗病性研突

通过PCR从苋属植物千穗谷(Amarathus hypochondriacus)的总DNA中扩增出苋菜抗菌肽(Ah—AMP)的核基因片段，序列分析结果表明该基因长261bp，编码一个由86个氨基酸组成的Ah—AMP前体多肽。在构建Ah—AMP基因的植物表达载体pBinAH916后，通过根癌土壤杆菌介导方法转化了烟草。转化再生植株和T1代转基因烟草的PCR和Southern blot分析表明，AA—AMP基因已整合到烟草的染色体中，并为单拷贝整合。T1代转基因烟草的Nouthern blot分析结果表明，AA—AMP基因在转基因烟草中至少在mRNA水平上能正常表达。对T0代转基因烟草进行烟草青枯病的抗病性试验，筛选出两株抗性较强的植株。对T1代抗青枯病的统计分析发现，其抗病性比起始品种SRl分别提高了2．24和1．62个级别；其病情指数比起始品种降低49．6％和37．3％。对黑烃病也表现一定的抗性，主要表现在推迟发病时间，减缓发病速度上。这些结果表明AA—AMP基因在植物抗病基因工程研究中可能是一个有潜在应用价值的基因。     

DNA分子标记在果树上的应用

介绍了在果树上常用的DNA分子标记的原理和特点，并对其在果树的分子遗传图谱和指纹图谱的构建，品种鉴别、系谱分析和分类，基因定位与分子标记辅助选择等方面的应用进行了综述。