Isolation,purification and characterization of a new organphosphorus hydrolase OPHC2

A bacterium with the capability of degrading organphosphorus, identified as Pseudomonas pseudoaicaligenes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65℃ and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pH stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.     

Cloning and roles of goldfish maternal factor β-Catenin cDNA in embryonic development

Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. β-Catenin, an important maternal factor in early embryogenesis, has been analyzed in its expression pattern and functions in this paper. We have cloned goldfish β-Catenin cDNA gene and compared it with zebrafish β-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern of β-Catenin by in situ hybridization and the roles of β-Catenin on embryonic development by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that β-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of β-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposterior axes, and leads embryos to die before larva get hatched.     

对甲苯磺酰氨基酸甲醇或2,2-联吡啶混配Cu(II)配合物的电化学合成及表征

采用金属Cu为“牺牲阳极”,在含配体对甲苯磺酰L-异亮氨基酸(H2L)的甲醇溶液中电解合成了[Cu(L)(2CH3OH)]配合物.当向含配体的甲醇溶液中加入2,2′-联吡啶时,电解合成了[Cu(L)(2,2’-bipy)]配合物.利用元素分析、红外光谱、紫外光谱、热分析对配合物进行了表征.测试了铜电极在溶液中的电化学行为和配合物的电化学性质.     

侧耳蛋白质和氨基酸含量测定与分析

对5种侧耳的子实体和菌丝进行蛋白质和氨基酸含量测定表明,侧耳蛋白质和氨基酸含量丰富,必需氨基酸种类齐全。但不同品种,不同部位差异较大。     

利用支持向量机预测生物膜蛋白类型

利用统计学习理论中的支持向量机(SVM)，基于氨基酸组分含量预测生物膜蛋白类型。使用文献中2059个训练集和2625个检验集膜蛋白序列数据，运用统计预测中的校准检验，留一法交叉检验和独立数据集检验方法进行分类预测。结果表明，SVM对膜蛋白类型预测具有明显的优越性，该算法对当前已有方法起到重要的补充作用。     

鸡蛋豆腐和牛奶豆腐的研制及营养评价

为了增加豆腐中的含硫氨基酸，提高大豆蛋白质的消化率，将牛奶、鸡蛋成分等添加到豆乳中，制成了不同品种的豆腐．营养评价的结果表明，添加鸡蛋或牛奶后，豆腐的蛋白质含量增加了20％—30％，豆腐新产品的营养价值提高．     

日本沼虾感光器Gqα基因的扩增及分析

采用分子生物学方法,提取日本沼虾感光器的总RNA,反转录成c DNA。根据Gq蛋白α亚基的保守序列设计简并引物,通过巢式PCR扩增出日本沼虾感光器Gqα基因的片段,并将该片段克隆到pMD 18-T载体上进行序列测定,结果显示此基因片段由369bp组成。翻译成氨基酸序列（123aa）,Blast搜索结果比较该片段氨基酸序列与美洲鲎、美洲龙虾、冈比亚按蚊等6种动物的Gqα氨基酸保守序列具有高度同源性（83.74％～95％）,其中与冈比亚按蚊的Gqα氨基酸序列同源性最高（95％）。     

人胎盘G—蛋白ab3a cDNA的克隆

为了获取全长的小分子G－蛋白Rab3a，以用于研究Rab3a与其他蛋白相互作用关系，本实验以人胎盘总cDNA为模板，PCR扩增到人Rab3a cDNA全编码区。产物回收后克隆于质粒pYESTrp2的BamHI/Xhol位点，测序结果表明，本实验获得的Rab3a cDNA包含了起始和终止密码子。与PCR引物设计的参照Rab3a比较有5个核苷酸变异，与翻译的氨基酸序列完全一致。由此表明本实验获得的Rab3a cDNA可用于进一步研究。     

黑鲷饲料蛋白源氨基酸平衡的研究

用黑鲷幼鱼肌肉的氨基酸组成作为幼鱼饲料中必需氨基酸需要量的标准模式,通过氨基酸指数（EAAI）和生物饲养试验结果相结合,对黑鲷幼鱼的9种不同蛋白源配比的饲料进行了比较研究,对黑鲷幼鱼配合饲料中氨基酸平衡和蛋白质营养价值进行了评估,筛选出2个高效饲料蛋白源配方;表明EAAI是鱼用饲料的蛋白质营养价值的评估和氨基酸平衡的研究的一种简便有效的研究方法。     

牧草与沼渣混合料栽培榆黄蘑的氨基酸分析与比较

测定并比较3种培养料栽培榆黄蘑的子实体氨基酸含量。结果表明，3种培养料栽培的榆黄蘑子实体含有丰富的必需氨基酸，支链氨基酸，精氨酸，谷氨酸。配方2，配方3培养料栽培的子实体必需氨基酸高于两种食用蛋白和配方1，支链氨基酸高于或接近两种食用蛋白。配方2（牧草沼渣混合料）支链氨基酸与芳香族氨基酸比值高于两种食用蛋白和4种农产品。表6，参5。