Inhibition of DNA restrictive endonucleases and Taq DNA polymerase by trimalonic acid C60

Activities of trimalonic acid fullerene (TMA C_60) on DNA restrictive enzymatic reaction were investigated by using two restrictive endonucleases Hind III and EcoR I and plasmid pEGFP-N1 with single restric-tive site for both enzymes. Meanwhile, TMA C60 was also tested to clarify its effects on polymerase chain reaction (PCR) with the catalyst of Taq DNA polymerase and the template of plasmid pEGFP-N1. The products from restrictive reactions or PCR were detected by agarose gel electrophoresis. It was found that the product amounts from restrictive reactions or PCR decreased significantly with addition of TMA C60. The inhibition by TMA C60 was dose-dependent and IC50 values for reactions of Hind III, EcoR I and PCR were 16.3, 6.0 and 6.0 μmol/L, respectively. Addition of two scavengers of reactive oxygen species (ROS), L-ascorbic acid-2-phosphate ester magnesium and sodium azide at the con-centrations of 2―10 mmol/L did not antagonize the activities of TMA C60 against PCR and two restrictive reactions. However, increase of Taq DNA polymerase amounts in PCR system antagonized the activities of TMA C60. These data implied that TMA C60 was able to inhibit the activities of the three above-mentioned enzymes involved in DNA metabolism, and that this inhibition probably did not correlate to ROS.     

稀土永磁材料RCO5及R2CO17晶场系数的计算

本文系统地计算了RCo_5、R_2Co_(17)(R为稀土元素)的晶场系数,并将所得的二阶项与J.E.Greedan和V.U.S.RAO的结果(他们只计算了二阶项)进行了比较.     

Numerical Modeling of Dendrite Growth in AI Alloys

Dendritic grains are the most often observed microstructure in metals and alloys. In the past decade, more and more attention has been paid to the modeling and simulation of dendritic microstructures.This paper describes a modified diffusion-limited aggregation model to simulate the complex shape of the dendrite grains during metal solidification. The fractal model was used to simulate equiaxed dendrite growth.The fractal dimensions of simulated AI alloy structures range from 1,63-1.88 which compares well with the experimentally-measured fractal dimension of 1.85; therefore, the model accurately predicts not only the dendritic structure morphology, but also the fractal dimension of the dendrite structure formed during solidification,     

老年人医院内下呼吸道感染的调查分析及预防

在我国医院感染的首位感染比例为23.3％-42.0％，在我院老年人下呼吸道医院内感染发病率也居首位，在治疗上较棘手，死亡率高，所以预防和控制下呼吸道感染是降低医院内感染的重要工作，为此，我们对78例老年人下呼吸道医院内感染患者的情况进行了调查与分析。现报告如下：     

大型高炉内解决高向加热均匀性的问题研究

1、采用高低灯头的方法，改善炭化室高向加热均匀性：将燃烧室内相邻两个火道中加热煤气的出口设在距碳化室底不同的高度处，这样可使上下加热情况稍有改善。此种方法的缺点是，由于火焰燃烧比较集中，使正对着燃烧火焰焦点处的炉墙容易产生局部过热。     

Nd:YAlO3双波长调Q脉冲激光腔内倍频的研究

本文构建了晶体双波长调Q激光腔内倍频的速率方程,通过数值求解,分析了不同腔长条件和倍频耦合条件对双波长脉冲激光时间特性和输出光子数的影响,通过仔细调整腔长和倍频耦合几何因子,理论上获得了均衡的红绿双波长调Q脉冲激光输出.     

聚晶金刚石切削齿散热电阻钎焊工艺的研究

采用散热电阻钎焊工艺对聚晶金刚石切削齿进行钎焊，钎缝的抗剪强度达４１７．５ＭＰａ，复合片的磨耗比下降率小于１０％。采用表面测温实时监控技术，使复合片上金刚石层受热的温度低于７００℃用复合片切削岩石，由此测定出复合片的耐磨性，这样增加了测试结果的可靠性，现场应用表明，用本工艺钎焊的聚晶金刚石切削齿制造的钻头质量稳定，基本无掉片现象。     

拓扑空间中集合的外部描述及其应用

文章给出了拓扑空间中集合的外部概念，研究了外部的性质，并用外部刻画了拓扑空间映射的连续性。     

描述裂纹愈合过程的内变量

在连续介质热力学框架内，以热力学第二定律为出发点，推导出裂纹愈合耗散不等式，确定了描述裂纹愈合过程的热力学内变量H的定义形式．裂纹愈合过程内变量的提出，不仅可以将裂纹愈合过程模型化，而且还可以建立裂纹愈合过程的演化方程和本构方程，为裂纹愈合过程的定量分析提供可能性．     

手性液晶的研究——Ⅸ．联苯羧酸酯类

合成了文题所述的十三个手性液晶化合物，经元素分析和红外光谱分析确证了其结构，并进行了相态、相变温度和电滞回线的测定，证实其为铁电液晶。