关于工程采购模式的改革研究

本文结合《高等数学》的教学实际,给出如何做好课堂教学工作的几点建议。     

指导发现式教学在篮球普修课教学中运用的实验研究

通过实验的方法,将"发现"式的学习理论应用于教学实践。实验表明:实验班的学生在篮球专项技术、操作思维能力、身体素质及运用篮球知识和技术解决问题的能力等方面明显高于对照班。"指导式发现教学"可使初学者尽快地掌握正确的技术动作,缩短技能形成所需要的时间;可使学生在学习技术动作的同时,深入系统地掌握理论知识;可培养学生运用知识进行观察、分析、判断能力,养成主动的学习习惯。     

新立城水库大坝安全管理信息系统

大坝安全监测信息实时查询、实时分析对大坝管理十分重要，本文简要介绍了新立城水库大坝安全管理信息系统的功能及结构，其良好的稳定性和可靠性，提高了水库管理的自动化水平。     

书法教育的功能说

书法是中华民族文化的结晶和象征,荟萃了中华民族文化的精髓,有着博大精深的文化内涵和巨大的文化魅力,书法教育是促进素质教育的必然要求和重要手段,书法教育在提高学生文化素质、智能素质、审美素质以及强化人格力量诸方面具有其它学科无法替代的功能。     

新型二异羟肟酸配合物催化PNPP水解的动力学研究

以3种含中心功能基(-OCH3)的二异羟肟酸开链冠醚过渡金属铜(Ⅱ)、锌(Ⅱ)和钴(Ⅱ)配合物作为水解酶模型,催化羧酸酯(PNPP)水解;研究了水解反应的动力学和机理,提出了水解反应的动力学模型。结果表明:在室温25℃条件下,这类配合物催化PNPP水解速率随着缓冲溶液pH值的增大而提高,表现出高的催化活性。     

毕业设计中软件工程的应用探讨

针对毕业设计过程中存在的问题,结合作者在指导计算机软件专业学生毕业设计过程中的经验和体会,本文主要研究学生在毕业设计时如何综合运用软件工程学的原理、技术和方法来组织和管理软件生产,以保证学生达到学以致用的目的.     

中老年人心血管功能对长期登山锻炼应答的实验研究

采用追踪实验法，对坚持登山锻炼的12名（45～66岁）中老年被试进行六年时间的追踪研究，观察心血管功能对登山锻炼的应答。结果表明：登山锻炼能够增大被试的肺活量，降低血压，减缓心率，促进血脂代谢的改善，提高心血管的适应力。同时发现：除LDL—C外，血脂代谢中的TG、TC和HDL—C水平的变化与个体状况有关，通用理论并不完全适用。     

Importance of NPA motifs in the expression and function of water channel aquaporin-1

The asparagine-proline-alanine sequences (NPA motifs) are highly conserved in aquaporin water channel family. Crystallographic studies of AQP1 structure demonstrated that the two NPA motifs are in the narrow central constriction of the channel, serving to bind water molecules for selective and effi-cient water passage. To investigate the importance of the two NPA motifs in the structure, function and biogenesis of aquaporin water channels, we generated AQP1 mutations with NPA1 deletion, NPA2 de-letion and NPA1,2 double deletion. The coding sequences of the three mutated cDNAs were subcloned into the mammalian expression vector pcDNA3.1 to form expression plasmids. We established stably transfected CHO cell lines expressing these AQP1 mutants. Immunofluorescence indicated that all the three mutated AQP1 proteins are expressed normally on the plasma membrane of stably transfected CHO cells, suggesting that deletion of NPA motifs does not influence the expression and intracellular processing of AQP1. Functional analysis demonstrated that NPA1 or NPA2 deletion reduced AQP1 water permeability by 49.6% and 46.7%, respectively, while NPA1,2 double deletion had little effect on AQP1 water permeability. These results provide evidence that NPA motifs are important for water per-meation but not essential for the expression, intracellular processing and the basic structure of AQP1 water channel.     

Bow-tie topological features of metabolic networks and the functional significance

Exploring the structural topology of genome-based large-scale metabolic network is essential for in- vestigating possible relations between structure and functionality.Visualization would be helpful for obtaining immediate information about structural organization.In this work,metabolic networks of 75 organisms were investigated from a topological point of view.A spread bow-tie model was proposed to give a clear visualization of the bow-tie structure for metabolic networks.The revealed topological pattern helps to design more efficient algorithm specifically for metabolic networks.This coarse- grained graph also visualizes the vulnerable connections in the network,and thus could have important implication for disease studies and drug target identifications.In addition,analysis on the reciprocal links and main cores in the GSC part of bow-tie also reveals that the bow-tie structure of metabolic networks has its own intrinsic and significant features which are significantly different from those of random networks.     

Investigation of adsorption of surfactant at the air-water interface with quantum chemistry method

Density functional theory （DFT） of quantum chemistry was used to optimize the configuration of the anionic surfactant complexes CH3（CH2）7OSO3^- （H2O）n （n=0-6） and calculate their molecular frequencies at the B3LYP/6-311＋G^＊ level. The interaction of CH3（CH2）7OSO3^- with 1 to 6 water molecules was investigated at the air-water interface with DFT. The results revealed that the hydration shell was formed in the form of H-bond between the hydrophilic group of CH3（CH2）7OSO3^- and 6 waters. The strength of H-bonds belongs to medium. Binding free energy revealed that the hydration shell was stable. The increase of the number of water molecules will cause increases of the total charge of hydrophilic group and S10-O9-C8 bond angle, but decreases of the alkyl chain length and the bond lengths of S10-O11, S10-O12 as well as S10-O13, respectively.