Glu101对保持中心蛋白正常构象作用的研究——光谱法研究稀土离子与八肋游仆虫中心蛋白作用对蛋白构象的影响

通过位点直接突变法将八肋游仆虫中心蛋白101位保守的Glu突变成Lys,得到突变蛋白E101K.利用疏水性探针TN S研究了突变对Tb^3＋与蛋白作用时蛋白构象变化的影响.结果表明,相对于钙饱和的蛋白,铽的饱和对蛋白构象变化的影响更大.同时,共振光散射研究表明,Glu101对保持蛋白适当的构象变化行为起着至关重要的作用.     

水稻16kDa醇溶蛋白启动子克隆及载体构建

水稻种子16 kDa醇溶蛋白是水稻种子成熟过程中,由16 kDa基因编码,16 kDa醇溶蛋白启动子调控,在胚乳中特异表达的蛋白质.以水稻基因组DNA为模板,通过PCR扩增技术得到16 kDa启动子片段,序列分析结果表明:获得的启动子片段的大小为931 bp,与已报道的该启动子序列相比较,其核苷酸序列同源性为99.9%.该启动子区域含有TATA-box,CAAT-box,GCN4基序,Prolamin-box等胚乳特异表达启动子所必需的正调控元件.利用该启动子构建了植物种子特异表达载体pC16 kDP.     

G蛋白偶联受体研究进展

G蛋白偶联受体(Gprotein-coupled receptors,GPCRs)是具有7个跨膜螺旋的蛋白质受体,是人体内最大的蛋白质家族。按GPCRs一级结构的同源性,主要分为A、B、C三族;按氨基酸序列的相似性以及与配基的结合情况GPCRs可以分为5个亚家族。本文就GPCRs的结构、分类、GPCRs的二聚化以及其固有活性等方面做了一些介绍。     

海洋生物黏附蛋白研究进展

海洋附着生物粘合蛋白的研究对于生物粘合剂的开发及海洋防污行业均具有重要的意义。综述了对贻贝足丝蛋白,藤壶胶蛋白以及管栖毛虫管胶蛋白的研究进展。3种不同生物的黏附蛋白具有某些相似性,同时又各有其结构特点,对其蛋白质结构,功能及粘合机制的研究既具有重要的学术意义也具有广阔的应用前景。     

植物细胞膜H^＋-ATPase对必需矿质养分的适应性

植物细胞膜H^＋-ATPase是初级转运蛋白，在各种离子和代谢产物的跨膜运输中起着重要的作用；介绍了植物细胞H^＋-ATPase的结构、生化特性、生理功能与调控机制；重点综述了细胞膜H^＋-ATPase的活性及其蛋白数量与基因表达对植物必需矿质养分的适应性。     

Comparing the biological coherence of network clusters identified by different detection algorithms

Protein-protein interaction networks serve to carry out basic molecular activity in the cell. Detecting the modular structures from the protein-protein interaction network is important for understanding the organization, function and dynamics of a biological system. In order to identify functional neighbor- hoods based on network topology, many network cluster identification algorithms have been devel- oped. However, each algorithm might dissect a network from a different aspect and may provide dif- ferent insight on the network partition. In order to objectively evaluate the performance of four com- monly used cluster detection algorithms: molecular complex detection (MCODE), NetworkBlast, shortest-distance clustering (SDC) and Girvan-Newman (G-N) algorithm, we compared the biological coherence of the network clusters found by these algorithms through a uniform evaluation framework. Each algorithm was utilized to find network clusters in two different protein-protein interaction net- works with various parameters. Comparison of the resulting network clusters indicates that clusters found by MCODE and SDC are of higher biological coherence than those by NetworkBlast and G-N algorithm.     

Preparation of 3-D porous fibroin scaffolds by freeze drying with treatment of methanol solutions

In this study, silk scaffolds with appropriate porous structures were prepared by adjusting solution concentrations and providing treatment with methanol solutions in the way of freeze drying. The effects of the preparation conditions on the microstructures and properties of the scaffolds were discussed. Fibroin solutions with different concentrations of 4, 6, 8, 10 wt% were used respectively to prepare the scaffolds. The effects of the addition of 20 vol% methanol before or after freeze drying to the 4 wt% fibroin solution were investigated. As demonstrated by Scanning Electron Microscope (SEM), the fib-roin scaffolds prepared without methanol had porous microstructures composed of thin sheets, and the sizes of the pores decreased with the increase of the fibroin solution concentrations, while the scaffolds prepared in the presence of methanol showed porous microstructures formed by fine-particle aggregates. The porosities and mechanical properties of the prepared fibroin scaffolds under different conditions were tested. The crystalline structures and conformations of the fibroin scaffolds were detected by Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD).     

蚕蛹蛋白纤维活性染料染色性能探讨

选用两类活性染料(Cibacron Red LS-2G,Cibacron Blue FN-R)对蚕蛹蛋白纤维长丝染色,讨论了工艺条件(Na2CO3的用量、NaCl的用量、温度)对两类活性染料固色率的影响.实验结果表明,两类活性染料对蚕蛹蛋白纤维均有较高的固色率,LS型活性染料的固色率大于FN型活性染料。NaCl对FN型活性染料的促染效果大于LS型活性染料,LS型活性染料在低盐条件下,也有较高的固色率。活性染料染色后,蚕蛹蛋白纤维长丝的强力损失小于10%.     

散射速率比浊测定高血压患者尿中微量蛋白

蛋白尿是肾脏受损的常见象征,以往因检验手段所限,不能测定出尿中的微量蛋白.而用速率散射比浊法可以测到ng级水平[1].我们经过对26例高血压病人尿液微量白蛋白、转铁蛋白、免疫球蛋白、α1微球蛋白(MA、TRU、IgU、α1M)的定量检测发现用常规方法检测无蛋白尿的高血压病人,尿中微量蛋白的排泄量已增加.为了观察高血压病对肾脏的早期损伤,用速率散射比浊法对高血压病人尿中四种项目进行定量检测结果如下.