饲喂两种中药配方对异育银鲫巨噬细胞免疫机能的影响

配制3种分别含0.03%强普素、0.5%中药组方和1%中药组方的饲料,投喂异育银鲫30 d后分离纯化头肾巨噬细胞,用于测定巨噬细胞吞噬力和巨噬细胞呼吸氧爆发.结果显示,0.5%中药组异育银鲫头肾巨噬细胞吞噬力和呼吸氧爆发最强,其次为1%中药组,与对照组相比差异显著(P＜0.05),而强普素组巨噬细胞吞噬力和呼吸氧爆发与对照组比无显著差异(P＞0.05).研究表明,0.5%和1%中药组方能够显著增强异育银鲫头肾巨噬细胞的吞噬力和呼吸氧爆发强度.对提高异育银鲫的免疫机能有显著作用.     

异育银鲫的卵细胞发生

本文对南京兴卫鱼场二龄异育银鲫的卵母细胞进行了观察,并对其卵细胞中液泡的性质及作用、卵黄积累的形式以及精孔器形态等问题作了描述和探讨。     

Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio

Gibel carp (Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immunofluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males.     

中草药对异育银鲫消化率影响的研究

以山楂、麦芽等中草药为原料,设计配方 和 ,在基础饲料中分别添加0.5%、1%、2%,并投喂异育银鲫(体重2g左右),2个月后,测定消化率.结果表明,异育银鲫的蛋白质消化率、脂肪消化率极显著地提高(P<0.01),碳水化合物消化率显著地提高(P<0.05),其中,中草药添加剂对蛋白质消化率的影响最为明显,脂肪次之,对碳水化合物消化率的影响较小.     

异育银鲫对糖利用性的研究：饲料糖含量对生长,消化吸？…

用糊精含量分别为５％,２５％,５０％的三种饲料饲养异育银鲫,研究异育银鲫对糖利用特点,每个循环过滤水族箱中放养４０尾鱼,每种饲中料喂两箱之鱼,饲养六周。摄食不同饲料的异育银鲫增重率有显著差异,２５％糊精组生长最快,５％糊精生长最慢。异育银鲫对三种饲料中糖的消化吸收率随着饲料糖含量的增加而极显著提高,粗蛋白消化吸收率虽然各组间差异不显著,但也有相同的趋势。     

温度对异育银鲫“中科3号”胚胎发育的影响

通过人工受精的方法获得异育银鲫"中科3号"(Allogynogenetic gibel carp"CASⅢ")受精卵,在水温(20±1)℃的条件下观察了"中科3号"胚胎发育各个阶段的形态特征,并将整个胚胎发育过程划分为受精卵、胚盘隆起、卵裂、囊胚、原肠胚、神经胚、器官发生和出膜等8个连续发育阶段,20个时期.此外,研究了18,20,22,24,26,28,32℃不同温度下异育银鲫"中科3号"受精率、孵化率和畸形率的变化情况.结果显示:异育银鲫"中科3号"胚胎发育的温度系数Q10值为2.05,适宜胚胎发育的温度范围为20~26℃,最佳孵化温度为24℃,温度与受精卵之间的回归方程式:y=19.465x-0.440 5x2-130.64(R2=0.978);温度与孵化率之间的回归方程式:y=34.581x-0.773 2x2-310.63(R2=0.9791);温度与畸形率之间的回归方程式:y=0.792 4x2-35.475x+393.03(R2=0.947);孵化温度与孵化时间呈负相关关系,异育银鲫"中科3号"胚胎发育生物学零度为14.8℃,有效积温为(627.26±6.67)℃·h.