杂交瘤细胞的无血清低蛋白培养

实现了杂交瘤细胞的体外无血清低蛋白培养。考察了血清浓度、基础培养基和接种密度对细胞生长的影响。在含5%血清培养基、1%血清培养基和无血清低蛋白培养基中适应生长的细胞,μ分别为0.68、0.45、0.44d-1,qGlc分别为13.4×10-9、13.1×10-9、19.2×10-9mmol/(cell·分别为39.4×10-9、44.1×10-9、37.7×10-9mg/(cell·d),YMAb/Xv分别为52.6×10-9、d),qMAb57.8×10-9、54.4×10-9mg/cell,YLac/Glc分别为2.0、1.95、2.0mmol/mmol。无血清培养中葡萄糖代谢途径不变,参与细胞维持代谢的葡萄糖比例增大。血清浓度降低,延迟期变长,最大活细胞密度下降。基础培养基影响细胞的最大活细胞密度,不影响生长速率。无血清培养基中细胞的接种密度选择在0.2×106～0.3×106cells/mL。     

从“适应理论”分析男子中长跑有氧与无氧训练

采用文献资料、对比等方法，对我国男子中长跑现状进行调查分析．从适应理论视角分析，现代中长跑有氧训练应与速度力量、技术训练相结合，提高速度素质．应在大强度训练与频繁赛事之间增大有氧训练比例，以良好竞技状态完成无氧训练，从刺激、恢复两个方面提高训练质量．     

杂草繁殖方式的多样性及其对环境的适应

杂草是在人为和环境双重选择压力下演化而成的一类高度进化的植物类群，其繁殖方式多样，表现在种子产量、萌发、休眠、拟态、生命力、繁殖体传播方式等。作者以举例方式比较详细地论述了杂草多样的繁殖方式，并讨论其对人为干扰环境适应的意义。     

抗虫耐旱高产杂交棉湘文1号选育报告

长期以来,棉铃虫、红铃虫的危害,棉区干旱问题,一直是制约棉花增产的主要因素,因此选育出抗虫耐旱杂交棉新品种是当务之急．湘文1号杂交棉具有高产,铃大,吐絮畅等优点,又具有抗虫耐旱、耐病等重要特性．1选育经过1993年从种植泗棉2号棉花品种的田块中,发现变异单株,经三年的提纯复壮,表现出植株高大,茎杆光滑,叶     

城市化进程中新居民市民化的角色转换与心理适应

从我国城市化进程的现状出发研究新居民市民化,首先涉及的是农民角色和市民角色的界定问题,也涉及到城市化进程中新居民市民化角色转换及其心理适应存在的问题、发展过程以及影响因素,因此要积极促进新居民市民化的角色转变,并采取对策加强心理适应的引导。     

高科技行业员工知识更新应对与适应状况研究

本研究通过访谈、编制问卷以及问卷施测，考察了高科技行业员工如何应对迅速更新的行业知识和技术，以及组织支持、认知评价、应对和员工目前适应状况之间的关系。本研究的现实意义在于：为企业和员工更好地应对迅速更新的知识和技术，提供了有意义的参考信息。     

我国现代节水农业前沿技术研究与发展趋势

现代节水农业前沿技术围绕作物生理需水与用水、精量控制灌溉等领域,对现代节水农业前沿技术开展原创性研究。通过对水-土-植物关系、干旱条件下植物根信号传输和气孔反应的机制、干旱胁迫锻炼对植物超补偿功能的刺激等问题的研究,带来农业节水原理与技术的创新,促进节水农业新     

渗透调节物质在作物干旱逆境中的作用

干旱逆境下参与作物渗透调节的物质主要有无机渗透调节离子K 、Ca2 、Mg2 和有机渗透调节物质可溶性糖、脯氨酸、甜菜碱等.干旱逆境下作物积累渗透调节物质可以维持细胞膨压、促进光合作用、参与作物体内活性氧自由基的清除,提高逆境下作物的收获指数和产量.     

Sr-Nd isotope geochemistry of eolian dust of the arid-semiarid areas in China： Implications for loess provenance and monsoon evolution

Strontium (Sr) is a divalent alkaline earth element. Its ionic radius (0.113 nm) is slightly larger than that of calcium (0.099 nm), and Sr thus substitutes isomor- phously for Ca in many minerals. Neodymium (Nd) is a rare earth element. Its chemical prop…     

Some evidence of drying trend over northern China from 1951 to 2004

The surface wetness index, Palmer drought sererity index and the retrieval of soil moisture over China were calculated using monthly precipitation and monthly mean surface air temperature. Based on the contrast analysis of the variation of the above three indices and precipitation, the dry/wet spatio-temporal pattern of northern China in the last 54 years was revealed, and the evidence of drying trend over northern China was analyzed, especially. The results show the following four facts: (1) The drying trend is the main characteristic of the eastern part of Northwest China and the central part of North China since the 1980s and it was enhanced in the last 15 years mainly due to the precipitation decrease and the temperature increase; (2) During the last 54 years, there was only one dry/wet shift at the interdecadal scale occurring in the eastern part of Northwest China and the central part of North China in the late 1970s, which was related to 1977/1978 global abrupt change, whereas there were three shifts in Northeast China, one was in the mid 1990s and the other two were in 1965 and 1983, respectively; (3) Unlike the variation trend of other subregions of northern China, the western part of Northwest China is currently located in a relatively wetting period, which is weak-ened due to the temperature increase; (4) The extreme drought frequency is obviously increasing in the eastern part of Northwest China, the central part of North China and Northeast China since the 1980s, which is closely related to the precipitation decrease and temperature increase in these subregions.