微生物连续培养过程中多态现象和振荡行为分析

对以甘油为底物的微生物连续培养法生产1,3－丙二醇模型进行研究,得到系统存在Hopf分叉,并给出了分叉值,绘出了周期解图,形同时分析了静态分叉点类型,给出了多态现象存在区域,上述分析与实验结果是相符的,从理论上解释了微生物连续培养过程中的多态现象和振荡行为。     

陕北鸡血清酯酶多态现象的初步研究

采用垂直平板聚丙烯酰胺凝胶电泳法(PAGE)检测陕北鸡血清酯酶,结果发现:ES-1区域有两个区带A、B表现为AA、AB、BB三种基因型,ES-1B的基因频率高于ES-1A的基因频率;ES-2域均表现为有带( )型.陕北鸡血清酯酶多态与其他鸡种相比较有其种的特异性;其遗传多样性指数为0.4657,表明该品种有很大的选育潜力.     

F-MSAP： A practical system to detect methylation in chicken genome

By replacing radiation with fluorescent system in the technique of methylation sensitive amplified polymorphism （MSAP） and optimizing reaction conditions, a modified technique to detect DNA methylation called F-MSAP （fluorescent labeled methylation sensitive amplified polymorphism） was developed. In the present study, cytosine methylation patterns of genomic DNA were investigated in two inbred chickens and their F1 hybrids. Three types of methylation patterns were observed in each individual, namely fully methylated, hemi-methylated or not methylated types. The average incidence of methylation was approximately 40%. The percentage that the F1 hybrid individual inherits the methylation for any given sites from either/both parent amounted to 95%, while the percentage of altered methylation patterns in F1 individual was only 5%, including 14 increased and 12 decreased methylation types, demonstrating that F-MSAP was highly efficient for large-scale detection of cytosine methylation in chicken genome. Our technique can be further extended to other animals or plants with complex genome and rich in methylation polymorphism.     

流行我国马牛的三个伊氏锥虫株的研究 ——Ⅱ.伊氏锥虫多态现象

首次报导流行我国马牛的伊氏锥虫株存在多态现象,描述了一种称作变异型(vartantform)的新形态.经过小鼠的连续传代和涂片以及单克隆接种小鼠的观察,证明这种变异型个体在流行我国的伊氏锥虫株中是一种正常的形态.     

我国东部沿海地区黑腹果蝇醇脱氢酶座位的多态性和渐变群研究

应用标准琼脂糖平板电泳技术,对我国东部沿海地区9个地方黑腹果蝇自然群体的醇脱氢酶座位的等位基因频率进行了测定。结果发现,在全部的群体中,该座位皆是多态的,且基因频率表现出纬度渐变群分布。还对这种多态性和渐变群形成的可能机理进行了讨论。