Transformation of the salt-tolerant gene of<Emphasis Type="Italic">Avicennia marina</Emphasis> into tobacco plants and cultivation of salt-tolerant lines

Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions.     

Improving the precision of the keyword-matching pornographic text filtering method using a hybrid model

With the flooding of pornographic information on the Internet, how to keep people away from that offensive information is becoming one of the most important research areas in network information security. Some applications which can block or filter such information are used. Approaches in those systems can be roughly classified into two kinds: metadata based and content based. With the development of distributed technologies, content based filtering technologies will play a more and more important role in filtering systems. Keyword matching is a content based method used widely in harmful text filtering. Experiments to evaluate the recall and precision of the method showed that the precision of the method is not satisfactory, though the recall of the method is rather high. According to the results, a new pornographic text filtering model based on reconfirming is put forward. Experiments showed that the model is practical, has less loss of recall than the single keyword matching method, and has higher precision.     

关于"线性空间”教材处理方法的探讨

通过"概念的引入,定理的证明,例题的讲解,内容的归纳和提炼”,谈关于线性空间教材处理的方法.     

一种新的肼基硫代酸苄酯的合成及荧光性质

合成了一个新的化合物,4-(N,N-二乙酰氧乙基氨基)苯甲醛缩肼基硫代酸苄酯,运用FT-IR,1H-NMR,UV-Vis,元素分析等进行了表征分析,测定了该化合物的荧光性质,从理论上分析了该化合物"反常"的双重荧光现象。     

简便实用的转抗除草剂基因后代植株鉴定方法--种子萌发测定法

种子萌发法是检测外源抗性基因在转基因植物中的表达和分析外源抗性基因在转基因植物中遗传行为的简单而直接的方法,以转ｂａｒ基因燕麦后代Ｒ２种子为材料,用不同体积分数的除草剂Ｃｈａｌｌｅｎｇｅ处理种子,萌发的幼苗经ＰＣＲ和Ｓｏｕｔｈｅｒｎ ｄｏｔ ｂｌｏｔ鉴定,发现在０．０３％的除草剂上萌发的幼苗,部分不含ｂａｒ基因,而在０．０７％和０．０９％的除草剂上萌发的幼苗,均含ｂａｒ基因。用０．０７％的除草剂测     

PC管桩在宁德车站软基处理中的应用

分析与总结了宁德车站中PC管桩的设计与施工中的重点，提出了PC管桩在实际运用中的一些建议。     

基于双尺度连续小波变换的二相编码信号识别

针对信号和噪声小波变换的模极大值在不同尺度上表现出截然不同的性质,提出一种利用粗细定位相结合的思想对二相编码信号奇异点实现精确定位的方法。该方法采用大尺度上的模极值点进行粗略定位奇异点出现的范围,并据此去除小尺度上的伪极值点,用小尺度上的模极值点精确定位奇异点出现的时刻。实验仿真比较了几种不同小波基函数下的算法性能,结果表明,该方法在采用db3小波基下对二相编码信号突变点的识别具有很好的效果。     

基于RBF神经网络的井眼方位角误差建模及补偿

对于陀螺钻井测斜技术而言,由于惯性器件本身误差及井下恶劣条件带来的干扰均具有复杂性,误差补偿是影响测量精度的关键因素。在阐述陀螺测斜原理、分析测斜系统可能产生的各种误差的基础上,进行了测斜仪转台实验,并采用神经网络中的径向基函数理论建立了井眼方位角误差模型,将其建模性能指标与双线形插值建模指标进行综合对比,结果表明RBF网络建模时间短、拟合性能好、预测能力强、补偿后方位角误差得到有效抑制、补偿精度高,各项指标均优于双线形插值方法。     

基于自抗扰控制器的永磁同步电机速度估计

利用自抗扰控制器理论,提出了一种新颖的永磁同步电动机无位置传感器矢量控制系统的转速估计方法。将转速和d轴电流对转矩电流环的耦合作用以及转动惯量的变化看作转矩电流环的扰动量,由自抗扰控制器将其观测出来并加以补偿,从而估计出电机实际转速。仿真表明在0r/min到1500r/min的调速范围内,本方法都能将转速准确地估计出来,并且对负载和转动惯量的变化具有很强的鲁棒性,系统具有良好的动静态性能和抗干扰能力。     

沥青低温指标试验方法及影响

沥青混凝土面层温度开裂是路面破坏的主要形式之一.低温开裂有两种形式:一种是由于气温骤降造成面层温度收缩,在有约束的沥青层内产生的温度应力超过沥青混凝土的抗拉强度时造成的开裂;另一种是温度疲劳裂缝.在我国还普遍存在第三种形式,那就是由于半刚性基层的收缩(温缩和干缩)而引起的面层开裂.在低温条件下沥青混凝土脆化,在荷载作用下容易产生开裂.SHRP研究表明90%的低温抗裂性能都是由沥青来提供的,所以选择低温性能优越的沥青将能很好地控制低温裂缝的产生.