有斑百合叶片的组织培养

试验采用红色顶生花有斑百合为试材，取其叶片进行组织培养，在MS＋2．4－D1．0ml／L＋6BA0．5mg／L＋蔗糖30g＋琼脂娄7g的培养基上进行培养，形成愈僵伤组织有鳞茎丛，在MS＋2．4－D1．0mg／L＋蔗糖30g＋琼脂粉7g的培养基上进行培养，仅形成了愈伤组织，在MS＋6BA1.0mg/L IAA0.5mg/L＋蔗糖30g＋琼脂粉7g上进行培养，不经过愈伤组织，直接在外植体上形成了鳞茎丛，经过诱导生根，长成子幼苗，幼苗长至5cm左右，栽到蛭石与沙土的混合基质中培养进一步长成了植株。     

福建省山樱花的组织培养及植株再生

以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽 。在1／4MS＋BA1．0mg/L IBA0.1mg/L的培养基上，其丛生芽诱导率达87．5％；在继代培养中选择MS＋BA1．0mg/L IBA0.1mg/L GA30.3mg/L或KT1.0mg/L NAA0.1mg/L BA30.3mg/L培养基，不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果，当芽伸长在3－4cm时，切下置于生根培养基1／2MS＋NAA1.0mg/L IBA1.0mg/L BA0.75mg/L中，生根率达90％以上，移栽成活率达95％。     

不同分离培养基对检测废水中微生物种群多样性的影响

用 3种不同培养基 (YPG、LB、WW)从焦化废水处理系统中分离培养细菌 ,并用 ERIC-PCR指纹分析的方法 ,对分离物的种群多样性进行了比较研究 .同一悬浮污泥样品在 YPG、LB和 WW培养基上的活菌计数结果分别为 1 .6× 1 0 6 CFU/ ml、7.0× 1 0 5CFU/ ml和 9.8× 1 0 5CFU/ ml.选取最适稀释度 (1 0 - 4) ,分别从 YPG、WW和 LB三种不同培养基平板上分离了 5 6、3 9和 60株菌株 .用 ERIC-PCR指纹图的方法分析这 1 5 5株菌株的多样性 ,结果显示 ,废水培养基 (WW)的选择性最强 ,3 9株分离物只显示 1 2种不同的指纹类型 ;而 YPG培养基的选择性相对较低 ,5 4株分离物中共有 3 0种不同的指纹类型 ,多样性明显 ;LB培养基上因有 2 8%的小菌落无法用 ERIC序列扩增 ,故不能用此方法对其多样性作有效评价 .至此 ,本次实验就分离焦化废水处理系统中的细菌及对分离物进行分子多样性的研究而言 ,选用 YPG培养基最合适 .     

环境及营养条件对阿佛曼菌(Streptomyces avermitilis)产阿维菌素的影响

阿维菌素是一类具有杀虫特性的大环内酯类抗生素。本文系统研究了环境条件包括温度、初始pH和接种量和培养基组成包括淀粉和氮源浓度对阿维菌素产量的影响，在单因素实验的基础上，采用U8（8^5)均匀设计方案，得到各因素的最佳组合。经实验验证，当培养温度为28℃，初始培养基pH为7.2，接种量为3％，淀粉浓度为70g/L，氮源浓度为53g/L，阿维菌素产量高达3089.14μg/mL。     

球形芽孢杆菌产毒条件的研究

球形芽孢杆菌(Baci Llus Sphaericus)在蚊幼孳生地能自然繁殖,我们从盐城郊区的污水塘中分离获得BS—1012,通过对库蚊、伊蚊及按蚊的生物学测定而知,其毒性高于标准株BS—1593,通过血清学试验证明其血清型为H_(5a5b)。本文主要研究氮源,碳源,无机盐,pH,温度、培养方法对BS—1012生长发育及产毒能力的影响。寻找最适合其生长发育的培养基,便于生产及大面积推广应用。     

固态发酵酿造糟渣制备菌体蛋白饲料

用木霉、扣囊拟内孢霉和丝孢酵母固态发酵酱油渣、醋糟制备菌体蛋白饲料，培养料配比为酱油渣６０％，醋糟２０％，麦麸１０％，玉米面１０％，无机营养盐适量。经１２１℃，３０ｍｉｎ或１００℃，６０ｍｉｎ灭菌后，以１０％接种等量混合的固体菌种开放培养４２ｈ后，物料收率８２％左右，蛋白含量２４．６％，粗纤维含量２２．３％，每克干料中的生物量为３．５×１０９。     

几种重金属离子及类金属砷对烟草组织培养的影响

本文以烟草为材料，在培养基中添加不同浓度的三种重金属离子(Hg^2-、Pb^2 、Ag^ )及类金属离子砷(As^5 )，经组织培养，发现经处理的烟草生长发育均受到不同程度的抑制．而且叶绿素和可溶性糖含量均比对照降低，仅蛋白质含量在金属及类金属离子浓度低(5ppm、10ppm)时部分升高．其余均降低，当这些重金属及类金属离子在培养基中的难度达到500ppm以上时，烟草的生长发育全被抑制。     

Improved elastase production by Bacillus sp. EL31410—further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K(2)HPO(4), MgSO(4).7H(2)O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K(2)HPO(4) 0.206 g/100 ml and MgSO(4).7H(2)O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.     

Improved production of spiramycin by mutant Streptomyces ambofaciens

Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant, Streptomyces ambofaciens XC 2-37, produced 9% more spiramycin than the parent strain S. ambofaciens XC 1-29. The effects of soybean oil and propyl alcohol on spiramycin production with S.ambofaciens XC 2-37 were studied. The potency orS. ambofaciens XC 2-37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at 20 hours after incubation in fermentor fermentation The new process with S. ambofaciens XC 2-37 was scaled up for industrial scale production of spiramycin in a 60 m^3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of fermentation were improved by 42.9%.     

在发酵过程中铁离子抑止金霉素生产的原因和去除抑止作用的研究

<正> 在金霉素的发酵过程中,铁离子浓度的增加对金霉素的生产起显著的抑止作用。铁是怎样抑止金霉素的生产的?怎样去消除这种抑止作用?这些都是值得加以研究的问题。