哈尔滨荒山地区100万年来地理环境分析

本文在实验分析荒山第四纪地质剖面中沉积物的机械组成，石英颗粒表面特征，沉积物中部分化学元素含量变化的基础上，结合前人的研究成果，深入分析了荒山地区１００万年以来各时期的环境征及１００万年来古环境的演变过程。     

外环流气升式反应器发酵生产聚β-羟基丁酸酯

目的　提高Z5 G菌株发酵生产聚β 羟基丁酸酯(PHB)产量。方法　采用改变营养配比、通气条件,研究了Z5 G菌在3L外环流气升式反应器中的最佳发酵工艺条件。结果　表明该菌株在C源N源配比为4∶1,发酵前期通气量为1L/L·min-1,后期为0 7L/L·min-1,发酵时间为36h,PHB发酵产率可提高10 7%。结论　Z5 G菌株在外环流反应器中发酵PHB比机械搅拌反应器产率高,发酵周期短,从而降低了能耗,对工业化发酵生产PHB具有实用意义。     

GCC box in<Emphasis Type="Italic">Arabidopsis PDF1.2</Emphasis> promoter is an essential and sufficient cis-acting element in response to MeJA treatment

The expression of Arabidopsis PDF1.2 gene isregulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression o fPDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDFI.2 gene. Secondly, the upstream -300— -243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the -300— -243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDFI.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box in PDFI.2 is an essential and sufficient element to confer MeJA induction.     

Self-assembled structure of alkyloxy substituted benzoic acid methyl ester on HOPG：An STM study

Self-assembled structures of 3,4,5-tris-dodecy-loxy benzoic acid methyl ester (E12), 3,4,5-tris-tetradecy-loxy-benzoic acid methyl ester (E14) and their mixture (E12/E14) have been studied on HOPG by scanning tunneling microscopy (STM). Dimer-like patterns induced by dipole-dipole interaction are observed in E12 and E14 monolayers. The molecules form close-packed rows and interdigitate with the alkyl chains in adjacent molecules. The structural differences are proposed to be from the different length of substituted alkyl chains. Owing to similar adsorption energy, phase separation is observed in the E12 and E14 mixed adlayer with different domains.     

植物激活剂苯并噻二唑(BTH)

概述了植物激活剂的概念和基本特点,苯并噻二唑(BTH)完全满足这些基本特征,是开发最成功的典型植物激活剂,较为详细的介绍了BTH的基本特性、作用机制、使用方法、应用范围和化学合成方法及其环境行为,并分析了该植物激活剂的应用前景和使用中可能存在的问题．     

Relationship between Earth-Sun-Moon 1000 Ma ago： Evidence from the stromatolites

Stromatolitesareusuallyinterpretedastheorgano-sedimentarystructurescausedbythegrowthandmetabo-lismofmicro-organisms,principallycyanobacteriaandprecipitatedmineralgrainstrappedtothestickylay-ers[1—5].Asthesunlightisstrongindaytime,thealgaefila-mentsshowstrongactivityinphotosynthesisandgrowtowardsunlight.Ontheotherhand,asthesunlightisweakatnight,thealgaefilamentsshowinactivityinphotosyn-thesisandgrowhorizontally.Duringthecourseofgrowth,thealgaewillproducesomeglutinoussecretionwhichcancatchsedim…     

说"情趣"

我国古代文论对"情趣"的标树较晚,明清时期出现将"情"与"趣"纳入到诗歌审美质性要素系统或创作主体要素系统中的立论.但实际上,早从唐代开始,不少文论家就已经将"情趣"视为了"趣"的审美表现形态之一,对"情"与"趣"相互间的关系予以了多方面的考察.我国古代曾出现过很多"趣"的称名,其中,不少都可归入"情趣"这一审美形态中.     

Homoharringtonine induces apoptosis of endothelium and down－regulates VEGF expression of K562 cells

Homoharringtonine (HHT) has currently been used successfully in the treatment of acute and chronic myeloid leukemias and has been shown to induce apoptosis of different types of leukemic cells in vitro. Emerging evidence suggests that angiogenesis may play an important role in hematological malignancies, such as leukemia. However, whether HHT can relieve leukemia by anti-angiogenesis is still unknown. We investigated the anti-angiogenesis potential of HHT with the human umbilical vein endothelial cell line (ECV304) and leukemic cell line (K562) in vitro. Cellular proliferation was determined by MTT assay and apoptosis was analyzed by flow cytometry, The mRNA expression of vascular endothelial growth factor (VEGF) was assessed by RT-PCR and VEGF protein production was detected by Western blot. Inhibition of cell proliferation and induction of apoptosis by HHT were discovered in ECV304 cells, and appeared in a dose- and time-dependent manner, Also, treatment with HHT caused down-regulation of VEGF mRNA expression in K562 cells in similar dose- and time-dependent manner and inhibition of VEGF protein production in K562 cells in response to the enhancing concentration of HHT. The results demonstrated that HHT could also induce apoptosis in endothelium and down-regulate VEGF expression in K562 cells. In conclusion, we believe HHT has anti-angiogenesis potential and speculate that HHT might exert its anti-leukemia effects via reduction of angiogenesis.     

深海沉积物样品中古菌的16S rDNA分析

利用多管采样器,在太平洋西经177°42'20",北纬10°35'06"的位置上,从水深5774 m的海底获得深海沉积物样本,现场提取DNA进行保存.以此DNA为模板,利用古菌16S rDNA特异引物扩增出样品中古菌的16S rDNA,将扩增所得的16S rDNA进行克隆,建立了该样品中古菌的16S rDNA文库.对16S rDNA扩增片段进行了RFLP分析和序列分析,结果表明这些古菌在系统进化树上的位置靠近,属于Crenarchaeota中的Ⅰ类海洋古菌类群.     

一种新的肼基硫代酸苄酯的合成及荧光性质

合成了一个新的化合物,4-(N,N-二乙酰氧乙基氨基)苯甲醛缩肼基硫代酸苄酯,运用FT-IR,1H-NMR,UV-Vis,元素分析等进行了表征分析,测定了该化合物的荧光性质,从理论上分析了该化合物"反常"的双重荧光现象。