miRNA与子宫内膜容受性

microRNAs(miRNAs)是一种内源性非编码小RNA,以完全或非完全互补的方式与其靶mRNA相结合,通过影响靶基因转录或翻译来调控细胞发育、分化、增殖、凋亡及疾病的发生发展等.在胚胎着床的子宫内膜容受性形成及蜕膜化过程中,胚胎滋养层细胞对子宫内膜的有控性侵袭是胎儿正常发育的基础.虽然,目前对miRNA与子宫内膜容受性的关系仍不十分清楚,但是已有一些研究表明,在正常妊娠胎盘、绒毛和正常子宫内膜中,一些miRNA与靶基因存在一定的的互作关系.本文简要综述了miRNA与子宫内膜容受性相关的研究进展.     

PTTG基因对人垂体瘤细胞侵袭能力的影响

目的：本研究的目的在于PITG基因过表达及沉默对人垂体瘤细胞侵袭力的影响,探讨其与垂体瘤细胞侵袭性的关系及调控机制。方法：用脂质体转染携带PTTG基因的表达质粒,构建过表达PTTG基因的垂体细胞株,观察细胞形态。另外我们运用脂质体转染PITGsiRNA对垂体瘤中的PITG基因进行干扰;利用Western blot技术检测其基沉默效应;通过Transwell小室法测定垂体瘤细胞侵袭能力的改变。结果：转染PITG siRNA后,垂体瘤细胞中PTIG的mRNA转录和蛋白表达受抑,显著低于未经处理的垂体瘤细胞（P〈0.01）。利用PTTGsiRNA对PTTG进行RNA干扰后,垂体瘤细胞的侵袭能力下降。结论：（1）在正常垂体细胞中过表达PTTG基因也可以引起细胞癌变,增强侵袭能力。（2）设计的PTTG siRNA能有效抑制体外培养垂体瘤细胞中PTTG的mRNA转录和蛋白表达水平,实现其基因沉默效应。PTTG与人垂体瘤的侵袭能力密切相关,其表达水平下降导致细侵袭能力降低。     

A simple and effective method for total RNA isolation of appressoria in Magnaporthe oryzae

Appressorium formation is an important event in establishing a successful interaction between the rice blast fungus, Magnaporthe oryzae, and its host plant, rice. An understanding of molecular events occurring in appressorium differentiation will give new strategies to control rice blast. A quick and reliable method to extract total RNA from appressorium is essential for studying gene expression during appressorium formation and its mechanism. We found that duplicate film is an efficient substratum for appressorium formation, even when inoculated with high density conidia. When inoculated with conidia at 1 × 106 ml^-1, the percentages of conidium germination and appressorium formation were （97.98±0.67）% and （97.88±0.45）%, respectively. We applied Trizol before appressorium collection for total RNA isolation, and as much as 113.6 lag total RNA was isolated from the mature appressoria at 24 h after inoculation. Functional analysis of two genes, MNH6 and MgATG1, isolated from the cDNA subtractive library, revealed that the quantity of RNA was good enough to construct a cDNA （complementary DNA） library or a cDNA subtractive library. This method may be also applicable for the appressorium RNA isolation of other pathogenic fungi in which conidia differentiate into appressoria in the early stages of host infection.     

一类RNA二级结构的计数

应用组合计数技巧研究了一类RNA二级结构的计数问题,给出了序列[1,n]的二级结构数和序列[1,n]上恰有k个基对的二级结构数的精确表达式.     

探讨基于平衡能的禽流感病毒结构分析

该文针对高致病禽流感病毒中的H5N1亚型,利用平衡能的方法,从二级结构的角度,对19株病毒做出功能分类,针对其所包含的八个片段,进行关联分析,对于不同片段对病毒的生物特性的影响做出解释。     

RNA结合蛋白与人类男性不育

本文概括了控制精子正常产生的诸多候选基因在染色体上的定位、演化及其编码的 RNA 结合蛋白与人类男性精子发生之间关系研究方面的最新进展,为人类男性不育症的治疗和计划生育的顺利推行奠定了坚实的理论基础并展示出光明的前景。     

RNA生物信息相关软件概述

随着功能性RNA在分子生物学研究的不断深入,出现了很多实用的RNA分析软件,他们功能各异,各具特色,为功能性RNA分子的结构研究提供了许多帮助。本文依据他们的功能介绍了几款较常用的RNA分析软件。     

Identification and characterization of two novel box HACA snoRNAs from Schizosaccharomyces pombe

Through constructing a specialized cDNA library based on small RNAs isolated from partial purified nuclei of Schizosaccharomyces pombe, two novel noncoding RNAs, termed Sp15-70 and Sp18-61, have been identified. Bioinformatics analysis reveals that both the novel RNAs possess a typical secondary structure of box HACA snoRNA and antisense elements to rRNAs. According to the relationship between the structure and function of box HACA snoRNA, Sp15-70 was predicted to direct pseudouridylation in 25S rRNA at U2401 and U2298; Sp18-61 was predicted to direct pseudouridylation in 18S rRNA at U208 and 25S rRNA at U2341. The four predicted pseudouridylation sites were all verified experimentally by the CMC-primer extension analysis. Both Sp15-70 and Sp18-61 were encoded by single copies which were located in the intergenic regions between the CDS of two protein-coding genes on chromosome Ⅰ and Ⅲ of S. pombe, respectively. Putative TATA-like elements can be found upstream from the 5′ end of these snoRNA genes, suggesting that they could be transcribed from their own promoters. Comparison of the two snoRNAs and their functional homologues in diverse organisms reveals that extensive recombinations among different snoRNAs have occurred during the evolution from their primitive progenitors.