锯缘青蟹抱卵蟹孵化的初步研究

选择性腺发育好的雌蟹 ,经催产后大部分个体在 2～ 14d内即可抱卵 ,抱卵率通常在 6 5 %左右 .抱卵的青蟹在水温 2 3～ 2 9℃ ,盐度 2 5～ 30下 ,经过 10～ 19d即可孵出幼体 .水温高 ,孵化时间短 ;水温低 ,孵化时间长     

大腹圆蛛包卵丝的化学组成与物理机械性能

分析了大腹圆蛛包卵丝的氨基酸组成，包卵丝中极性氨基酸含量较高，小侧基氨基酸含量较少，利用SEM及计算机图像处理技术分析了大腹圆蛛包卵丝的形态结构特征，包卵丝的断面形状基本为圆形，包层包卵丝的细度约为内层包卵丝的2倍，并且外层包卵丝的纤维断面内有许多无规分布的纳米级孔隙，经冷冻干燥后，孔隙率增加了2倍左右，在此基础上，研究了大腹圆蛛包卵丝的拉伸机械性能，光泽和热性能，初步探索了性能的形成机理，大腹圆蛛内层包卵丝具有比其他功能蜘蛛丝和丝素纤维好得多的力学性能，其强度高，伸长率大，韧性好，包卵丝在光泽和热性能方面与蚕丝丝素纤维有一定的差异。     

两卵形弧间的等距对立

本文目的是研究两卵形弧间的等距对应，所得的主要结果是下列两定理：设Ｃ和Ｃ＾＊（是两处正则的卵形线，它们的长度相等，ψ：Ｃ→Ｃ＾＊是等距映射，则在Ｃ上有４个不同的点Ａ１，Ａ２，Ａ３，Ａ４使Ｃ在Ａｉ的曲率等于Ｃ＾８在ψ（Ａｉ）（ｉ＝１，２，３，４）。设Ｃ和Ｃ＾＊是都是以Ａ、Ｂ为端点的卵形弧，它们的长度相等；ψ：Ｃ→Ｃ＾＊是等距映射使ψ（Ａ）＝Ａ；Ｍ以Ｃ上一点；对于ＡＭ上任一点Ｐ有ｋ（Ｐ）≥ｋ（ψ（ｐ）     

卵菌菌丝顶端钙调素的免疫组织化学定位

采用胞壁水解酶（Ｄｒｉｓｅｌａｓｅ２ｍｇ·Ｌ－１）和甲醇（－２０℃），分别对卵菌（Ｓａｐｒｏｌｅｇｎｉａｆｅｒａｘ）菌丝细胞壁及细胞膜进行通透，然后用免疫组织化学法对菌丝钙调素（ＣａＭ）的分布进行定位．结果表明ＣａＭ较密集地分布在菌丝顶端和在菌丝旁侧新形成的侧枝，亚顶端次之，基部菌丝几乎无特异性染色．由此提示，ＣａＭ可能参与卵菌菌丝顶端生长的调控．     

厦门卵索线虫生活史的初步研究

报道厦门岛西南部沿海寄生于蝗虫体内的厦门卵索线虫的生活史，对发育过程和发育各期的形态特征进行了较详细的描述。本种线虫生活周期为一年。     

五种蝗卵的形态记述

记述了网翅蝗科的黑翅竹蝗Ceracris fasciata fasciata,中华雏蝗Chrothippus chinensis,斑翅蝗科的黄翅踵蝗Pteruoscirta calliginosa,剑角蝗科的长角佛蝗Phlaeoba antennata和僧帽佛蝗Ph.infumata等五种蝗虫的卵囊形态和构造,卵粒的形态和显微结构。     

Nitric oxide produced by cumulus cells stimulates maturation of mouse oocytes

The effect of sodium nitroprusside (SNP), a donor of nitric oxide, on meiotic maturation of mouse oocytes was studied by injecting N^w-nitro-L-arginine methyl ester (L-NAME) intra-peritoneal (ip), a nitric oxide synthase inhibitor, or culturing oocytes in the medium supplemented with L-NAME or hypoxanthine (HX) to arrest the spontaneous oocyte maturation in vitro. The results showed that the inhibitory effect of L-NAME by injecting 10 mg/kg ip on extrusion of the first polar body only could be reversed by injecting 2.5 mg/kg SNP with L-NAME simultaneously (P < 0.05). Half an hour later ten mice died when given 10 mg/kg SNP ip. The treatment of some concentrations of SNP (10^–7, 10^–6, 10^–5mol/L) significantly stimulated meiotic maturation to metaphase Ⅱ stages in cumulus enclosed oocytes in the presence of HX. However, other concentrations of SNP (10^–8, 10^–4, 10^–3 mol/L) had no effect on HX-arrested oocyte meiotic maturation. The optimal concentration of SNP on CEOs had no effect on DOs. The dose of 10^–3 mol/LL-NAME demonstrated a significant suppression in formation of PB₁, but not in GVBD. This inhibition was reversed by the addition of SNP. These results indicated that the physiological levels of NO produced by cumulus cells could stimulate meiotic maturation of mouse oocytes both in vivo and in vitro.     

青虾的人工育苗试验

选择产自天然水域的抱卵虾作为亲虾进行室外小水泥池人工育苗试验,两口水泥池共32平方米共获0.8～1.2厘米的虾苗30万尾,平均每平方米水面产虾苗9300尾。