红花银桦的引种栽培试验研究

为了丰富柳州市的观花树种,于2008年2月至2012年12月在柳州市园林科学研究所羊角山苗圃和柳州市河西花卉公园进行红花银桦(Grevillea banksii var.forsteri)的引种栽培试验研究,观察红花银桦在柳州的生长特性。结果表明,红花银桦能适应柳州市气候环境,在柳州市的生长速度比较快,树高年平均生长量124cm,冠幅年平均生长量77cm,地径年平均生长量3.2cm,并且能正常开花,花色鲜艳美丽,花期长达8个月。红花银桦可以作为观花树种在柳州园林绿化中推广种植。     

90°弯道T型丁坝水力特性和数值模拟研究

利用雷诺平均Navier-Stokes(RANS)方程和k-ω涡粘紊流模型,研究在无丁坝、直线型丁坝、T型丁坝三种工况下90°弯道水流中的水力特性.研究结果表明:与直线型丁坝相比,T型丁坝下游形成的回流区长度约缩短15%,宽度约缩窄25%;对水面壅高、横纵比降的影响程度较小,且将横向环流移向河道中心,更加合理的减轻环流对岸边的水毁破坏;河床切应力突增率低于直线型丁坝,强度顺河道向下游衰退更快.综合比较,T型丁坝是一种优于直线型丁坝的坝型.     

粗粒含量对砂卵石土宏细观力学特性影响分析

摘 要: 砂卵石土粗粒含量对基坑及隧道围岩等稳定性影响较大,然而不同粗粒含量砂卵石土宏细观力学特性尚不明确。采用室内大型粗粒土三轴试验与数值三轴试验相结合的方法,对不同粗粒含量砂卵石土宏观及细观力学特性开展研究。研究结果表明：随着粗粒含量增加,砂卵石土的应力应变曲线表现为应变软化性;围压不变时,砂卵石土随着粗粒含量增加,应力峰值增大而达到峰值时的应变逐渐减小;建立了砂卵石土粗粒含量与内摩擦角和粘聚力等力学指标之间函数关系,随着粗粒含量的增加,砂卵石土的内摩擦角与粘聚力呈线性增大;提出了不同粗粒含量砂卵石土的接触模量、颗粒刚度比、摩擦系数、接触粘结强度等颗粒离散元细观参数。研究成果为砂卵石地层工程精细化设计及施工提供理论支撑。     

Distribution of lanthanum among the chloroplast subcomponents of spinach and its biological effects on photosynthesis： location of the lanthanum binding sites in photosystem Ⅱ

The effects of lanthanum at different concentrations on the related photosynthetic activities of Hill reaction, Mg^2＋-ATPase and Ca^2＋-ATPase in spinach chloroplast were studied. Experimental results showed that lanthanum can increase all the activities at suitable concentration （15-30 mg· L^-1）, however, it behaves toxically on them when over used （60 mg. L^-1）. To get an improved understanding of the mechanism of lanthanum effects on the photosynthesis of spinach, the different subcomponents in the chloroplast of the cultured spinach were isolated, and the content of lanthanum in each subcomponent was determined by ICP-MS. The results obtained indicated that among these different subcomponents, about 90% out of the total chloroplast lanthanum was located in photosystem Ⅱ （PS Ⅱ） while there was little lanthanum in photosystem Ⅰ （PS Ⅰ）. Moreover, size exclusion high performance liquid chromatography （SE-HPLC） coupled with online UV and ICP-MS detections was novelly used for locating lanthanum binding sites in PS Ⅱ proteins for the first time. It was found that lanthanum has two binding sites in PS Ⅱ： La associates with chlorophyll together with magnesium in PS Ⅱ by partly replacing magnesium and also shares the common binding sites of PS Ⅱ proteins together with the inorganic cofactors of calcium and manganese, influencing the process of photosynthesis.     

Expression,purification and spectra characterization of neuroglobin

The expression, purification and spectra characterization of recombinant human neuroglobin （NGB） are reported. The pET3a plasmid with the gene of NGB was transformed to E. coli BL21 （DE3） plys cells and expressed in TB culture medium. The results indicated that the expression amount of NGB is about 10 percent of the total protein in cells. The NGB protein was purified by ammonium sulfate precipitation, DEAE-Sepharose anion exchange column, Hiload 16/60 superdex 75 size exclusion chromatography and a Hiprep 16/10 Q FF anion exchange column, and a red soluble protein was obtained which showed a single band in electrophoresis. Electrospray ionization mass spectrometry （ESI-MS） showed that its molecular weight is 16930.0 Da. UV-spectra indicated that the reduced NGB has a strong absorption peak at 425 nm, and two weak peaks at 531 and 559 nm, which can be assigned to γ, β and α bands of porphyrin, respectively, and the oxidized NGB has a strong absorption peak at 413 nm which corresponds to the transition of π electrons in the porphyrin ring. The fluorescence maximal excitation wavelength is at 281 nm and its maximal emission wavelength is at 338 nm. CD spectra indicated that its secondary structure is a typical α helix, and has a positive peak at 410 nm induced by heme, The NGB protein is stable when the pH is higher than 4.     

应用于热光伏电池的一维Si/SiO_2光子晶体滤光器研究

光谱控制方法被广泛应用于热光伏系统以提高系统的热电转换效率.针对锑化镓(GaSb)电池热光伏系统的光谱控制要求,选用Si和SiO2设计了8层一维光子晶体滤光器结构;重点结合滤光器与高温辐射源辐射光谱功率分布和GaSb电池量子效率的匹配,对设计结构加以改进优化得到最佳匹配滤光器结构;采用光学镀膜技术加工了滤光器结构,并测试其光谱特性,根据测试结果计算滤光器的光谱控制效率、系统转化效率和能量密度.最后,测试了滤光器的抗高温性能.     

考虑偏平面屈服特性的三维耗散应力空间

天然沉积土大多处于复杂三维应力状态．建立岩土材料三维本构模型的方法（三维化方法）应满足热力学定律这一基本物理规律．本文通过引入与塑性剪应变相应的迁移应力,使基于耗散能增量函数建立的岩土材料本构模型能合理地描述偏平面的三维屈服特性．同时,给出了耗散应力空间应力张量与真实应力张量之间的关系表达式,使本构模型能在三维耗散应力空间中采用相关联的流动法则计算不同应力方向的塑性应变．其次,在热力学框架下对比分析了常见三维化方法的热力学本质,如直接引入强度准则的方法、g（θ）方法和变换应力方法等．说明了三维耗散应力空间与变换应力空间的等价性,验证了变换应力方法在热力学框架下的合理性,同时指出直接引入强度准则的方法和g（θ）方法的不合理之处．最后,通过模型预测值与试验值的比较,验证了建立的三维耗散应力空间及其等价的变换应力空间的适用性．     

不同植被恢复类型的沙地土壤中溶解性有机质演变特征

溶解性有机质(dissolved organic matter,DOM)是土壤有机碳的重要组成部分,可以有效地评价干旱区沙漠化土壤植被恢复状况.然而,对于不同恢复方式、不同植被类型(如草地、灌木丛、针叶林、落叶林等)沙地土壤中DOM的演变特征与光谱学特性缺乏认知.本研究以毛乌素沙地为例,采集3种土地利用类型(裸沙地、配...