欧洲生物信息研究所Ewan Birney访谈：寻求基因组之龙

当人类基因组计划于2003年完成之际,它给出了30亿个碱基对的DNA序列。之后,研究人员需要将那些序列数据翻译成对编码蛋白质基因有意义的信息,而这些工作是40多年来分子生物学研究一直关注的焦点。研究人员需要弄明白那些处于基因间或者基因中对编码蛋白质没有明显作用的、传统上视为“垃圾”的DNA有什么含义。     

医学分子生物学开放式实验改革初探

探索医学院校医学分子生物学实验教学的新模式。医学分子生物学开放式实验教学是一种创新而卓有成效的学习方式,充分调动了实验室资源,全方位提升了学生的素质,培养了具备扎实理论功底和实际动手能力的医学新人才。     

Molecular characterization and genetic analysis of Gnrh2 and Gthβin different ploidy level fishes

The gonadotropin-releasing hormone (GnRH) and the gonadotropin hormone (GTH) play crucial roles in regulating the gonadal development of the vertebrate. In this study, the Gnrh2, Fshβ and Lhβ cDNAs were cloned and characterized in red crucian carp, triploids and tetraploids, and their phylogenetic relations were comparatively analyzed. All the Gnrh2 cDNAs in different ploidy fishes encoded proteins of 86 amino acids, which consisted of a signal peptide, a GnRH2 decapeptide and a GnRH-associated peptide (GAP) linked by a proteolytic cleavage site (Gly-Lys-Arg). The GnRH2 decapeptide and proteolytic cleavage site were absolutely consistent among the three ploidy fishes, but the differences in signal peptide and GAP between diploids and tetraploids were fewer than those between diploids and triploids. It was presumed that the red crucian carp was the original maternal parent of tetraploids, so they had closer relationship. In addition, the Fshβ and Lhβ cDNAs of these three fishes encoded proteins of 130 and 140 amino acids, respectively. Compared with the molecules of other teleosts, the cysteine residues and potential glycosylation sites of Lhβ in these three fishes are fully conserved. However, most teleosts of Fshβ had 12 cysteine residues, while those of these three fishes were 13, and 12 of which might form six conserved disulfide bridges by utilizing the cleavage sites between the first and the second cysteine residues. Moreover, the lack of the second glycosylation site in Fshβ of these three fishes might influence the special structure and biological activities. On the other hand, the phylogenic tree analyses revealed that Gnrh2, Fshβ and Lhβ had similar phylogeny relationships among the cyprinids, which indicated that they were conserved in molecular structure and function during the evolution.     

达尔文学说问世以来生物进化论的发展概况及其展望

 综述了自达尔文学说诞生以来,生物进化论经历了孟德尔颗粒遗传理论、新拉马克主义、新达尔文主义直到现代综合进化论建立的发展历程。然而,综合论更面临着来自分子生物学新信息和古生物学重大新发现的挑战和发展机遇,由此产生了分子中性遗传漂变假说和三幕式寒武大爆发假说。     

2010年第10期科技人才招聘

中国科学院昆明动物研究所中国科学院昆明动物研究所动物毒素学科组招聘博士后（1名）。要求：40岁以下,具有生物学或药学相关专业博士学位;有较强的专业背景知识（生物化学与分子生物学、微生物学、     

分子生物学实验教学改革初探

分子生物学实验是生物专业本科生的一门专业必修课。根据我校本科生教学课程的情况,就课程内容更新、实验准备、实验时间和内容安排、教学方法改进、学生动手能力和科研创新意识培养以及实验考核方式完善等方面的教学经验进行了探索。     

The development of taste transduction and taste chip technology

Taste is one of important sensations. The primary taste sensations commonly are categorized as sweet, acid, bitter, salty and umami, of which various tastes are composed. The sensation of taste is initiated by the interaction of tas- tants with receptors and ion channels in the apical micro- villi of taste receptor cells (TRCs) when some sapid molecules (tastants) dissolve in saliva. Subsequently, through a cellular signaling pathway (TRC depolarization and Ca2+ release) gustatory signals a…     

转生长激素基因彩鲫(Colorful Curcain carp)的获得及检测

转基因育种技术加快了鱼类育种速度,已成为鱼类分子生物学研究的重点．以往用于转基因鱼研究的基因元件基本上都是来自哺乳动物,从基因安全的角度讲,构建全鱼“元件”的转基因鱼是转基因鱼市场化的前提．作为观赏鱼类的彩鲫,色彩丰富,体形优美,但其生长速度缓慢,通过转基因方法可望生     

实用生物信息科学

本书为《核酸和分子生物学》丛书的第15卷，至今这是唯一一本沟通生物信息学家和分子生物学家，即沟通生物数据分析计算法的开发者和使用者的一本书，本书从蛋白质序列的研究和预测结构的计算法开始，直接利用实验室获得的数据改进功能预测的方法，包括一系列在实验研究中，应用不同类型的生物信息科学法。