Isolation,purification and characterization of a new organphosphorus hydrolase OPHC2

A bacterium with the capability of degrading organphosphorus, identified as Pseudomonas pseudoaicaligenes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65℃ and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pH stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.     

CCEBC/EEAC 方法的定性分析

CCEBC/EEAC 方法是对电力系统暂态稳定性作量化分析的一个很有效的方法. 对CCEBC/EEAC方法进行了定性分析, 说明从几何观点出发, CCEBC/EEAC 方法中的 CCCOI-RM 可以看作是对系统模型的变量在权向量空间上的投影, 由此得到广义-轨迹. 由于电力系统的暂态过程可以近似地看作分时间段简单Hamilton系统, 为了给出CCEBC/EEAC方法的定性分析, 比较了两机无穷大系统的势能与 CCEBC/EEAC 能量. 计算结果表明两者相似. 弄清这两者之间的定性关系, 对于从数学上论证CCEBC/EEAC方法能够用于判定系统的暂态稳定性将起到重要作用. 通过定性分析, 也能更好地了解 CCEBC/EEAC 方法基于量化分析所揭示的一些重要现象, 如多摆失稳和孤立稳定域.     

Activation of proteinkinase ERK mediates induction of macrophage MMP-12 by OxLDL

The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of maerophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot analysis.Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner.OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12.These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway.     

A new self-adapting admission control algorithm for differential service in Web clusters

A new admission control algorithm considering the network self-similar access characteristics is proposed. Taking advantage of the mathematical model of the network traffic admission control which can effectively overcome the self-similar characteristics of tile network requests, through the scheduling of the differential service queue based on priority while at the same time taking into account various factors including access characteristics of requests, load information, etc. smoothness of the admission control is ensured by the algorlthm proposed in this paper. We design a non-linear self-adapting control algorithm by introducing an exponential admission function, thus overcomes the negative aspects introduced by static threshold parameters. Simulation results show that the scheme proposed in this paper can effectively improve the resource utilization of the clusters, while at the same time protecting the service with high priority. Our simulation resuhs also show that this algorithm can improve system stability and reliability too.     

Sm3+掺杂碱锌硼硅酸盐玻璃的稳定性

为了实现纳米半导体微晶在稀土Sm^3 掺杂碱锌硼硅酸盐基础玻璃中的受控成核、生长,系统研究了基础玻璃的SiO2与B2O3摩尔比以及BaO、ZnO、La2O3、Y2O3、Nb2O5等不同的网络中间体对玻璃稳定性的影响。研究表明：控制SiO2与B2O3摩尔比以及BaO、La2O3、Y2O3、Nb2O5在玻璃中的掺入量均能提高玻璃的形成能力,增加玻璃的稳定性;不同的组分对于玻璃稳定性的改善也具有不同的机制,但是并不影响玻璃的析晶产物。     

大倾角走向长壁开采"R-S-F"动态稳定性实验

通过平面应力走向、倾斜和三维可加载块体模型等手段,较系统地研究了大倾角煤层走向长壁工作面(倾斜布置)开采过程中矿山压力显现规律、围岩变形、破坏与移动特征和工作面支架与设备的下滑机理;分析探讨了顶板破断岩块运动、底板破坏滑移以及主要回采工序对支架或支护系统工作阻力和"R-S-F"(顶板-支架-底板)系统动态稳定性的影响;提出了现场工业性试验时需要注意和重点解决的关键技术问题。     

堤身蚁穴系统的结构及强度与稳定性研究

蚁穴系统是由洞穴通道和生化土体构成的复杂间断系统.由蚁酸与粘粒土拌合而成的蛋白泥胶,不仅具有良好的保温、保湿性能,而且对土体的强度和稳定性具有较大的影响.在干燥或潮湿状态下,泥胶对土体的强度和稳定性具有加强的作用;但在饱和状态下,随着其含量的增加,土体的强度和稳定性将降低,并分别呈反"S"型和"先缓后陡"下降型变化;特别是在水流的冲蚀或潜蚀作用下,易使土体结构崩解破坏,并最终导致地面塌陷积水和边坡失稳滑移.     

仿射非线性一般模型控制的鲁棒稳定性分析

针对基于仿射非线性系统的一般模型控制(GMC)方法进行了鲁棒稳定性分析。首先证明了如果模型存在一个严格Lyapunov函数,当模型与过程的失配在一定范围内时,则过程总是Lyapunov稳定的。由此推广到采用GMC方法的闭环控制系统,在给出其显式控制律的前提下,证明了满足一定前提条件下,采用GMC的闭环控制系统总是Lya-punov稳定的。     

原位聚合法制备聚苯胺/ 聚乙烯醇导电材料的研究

采用原位聚合方法合成了聚苯胺／聚乙烯醇导电复合材料,研究了反应体系中聚苯胺的质量分数和反应时间对复合材料电导率的影响,并且通过红外光谱和热重分析对其结构和稳定性进行了表征和分析．     

Effect of addition of poly（ethylene glycol） on electrical conductivity of poly（3,4-ethylenedioxythiophene）-poly（styrenesulfonate） hybrid

By mixing various concentrations of poly (ethylene glycol), a series of poly(3,4-ethylenedioxythiophene)-poly(styeenesuffonate) composite thin films were prepared.The electrical conductivity of the PEDOT-PSS/PEG thin films was measured by the four-pcobe method. Experimental results showed that the inclusion of poly(ethylene glycol) influenced the electrical conductivity of PEDOT-PSS film significantly. With the increase of PEG concentrations, the electrical conductivity sharply increased to reach a maximum and then slowly decreased down. Furthermore, the PEG molecular weight and environment temperature also played important roles on the electrical conductivity of PEDOT-PSS/PEG thin films. A good linear relationship was found between in σDC and T^-1/2 within the entire temperature range detected.