中老年人心血管功能对长期登山锻炼应答的实验研究

采用追踪实验法，对坚持登山锻炼的12名（45～66岁）中老年被试进行六年时间的追踪研究，观察心血管功能对登山锻炼的应答。结果表明：登山锻炼能够增大被试的肺活量，降低血压，减缓心率，促进血脂代谢的改善，提高心血管的适应力。同时发现：除LDL—C外，血脂代谢中的TG、TC和HDL—C水平的变化与个体状况有关，通用理论并不完全适用。     

Importance of NPA motifs in the expression and function of water channel aquaporin-1

The asparagine-proline-alanine sequences (NPA motifs) are highly conserved in aquaporin water channel family. Crystallographic studies of AQP1 structure demonstrated that the two NPA motifs are in the narrow central constriction of the channel, serving to bind water molecules for selective and effi-cient water passage. To investigate the importance of the two NPA motifs in the structure, function and biogenesis of aquaporin water channels, we generated AQP1 mutations with NPA1 deletion, NPA2 de-letion and NPA1,2 double deletion. The coding sequences of the three mutated cDNAs were subcloned into the mammalian expression vector pcDNA3.1 to form expression plasmids. We established stably transfected CHO cell lines expressing these AQP1 mutants. Immunofluorescence indicated that all the three mutated AQP1 proteins are expressed normally on the plasma membrane of stably transfected CHO cells, suggesting that deletion of NPA motifs does not influence the expression and intracellular processing of AQP1. Functional analysis demonstrated that NPA1 or NPA2 deletion reduced AQP1 water permeability by 49.6% and 46.7%, respectively, while NPA1,2 double deletion had little effect on AQP1 water permeability. These results provide evidence that NPA motifs are important for water per-meation but not essential for the expression, intracellular processing and the basic structure of AQP1 water channel.     

Bow-tie topological features of metabolic networks and the functional significance

Exploring the structural topology of genome-based large-scale metabolic network is essential for in- vestigating possible relations between structure and functionality.Visualization would be helpful for obtaining immediate information about structural organization.In this work,metabolic networks of 75 organisms were investigated from a topological point of view.A spread bow-tie model was proposed to give a clear visualization of the bow-tie structure for metabolic networks.The revealed topological pattern helps to design more efficient algorithm specifically for metabolic networks.This coarse- grained graph also visualizes the vulnerable connections in the network,and thus could have important implication for disease studies and drug target identifications.In addition,analysis on the reciprocal links and main cores in the GSC part of bow-tie also reveals that the bow-tie structure of metabolic networks has its own intrinsic and significant features which are significantly different from those of random networks.     

Investigation of adsorption of surfactant at the air-water interface with quantum chemistry method

Density functional theory （DFT） of quantum chemistry was used to optimize the configuration of the anionic surfactant complexes CH3（CH2）7OSO3^- （H2O）n （n=0-6） and calculate their molecular frequencies at the B3LYP/6-311＋G^＊ level. The interaction of CH3（CH2）7OSO3^- with 1 to 6 water molecules was investigated at the air-water interface with DFT. The results revealed that the hydration shell was formed in the form of H-bond between the hydrophilic group of CH3（CH2）7OSO3^- and 6 waters. The strength of H-bonds belongs to medium. Binding free energy revealed that the hydration shell was stable. The increase of the number of water molecules will cause increases of the total charge of hydrophilic group and S10-O9-C8 bond angle, but decreases of the alkyl chain length and the bond lengths of S10-O11, S10-O12 as well as S10-O13, respectively.     

高校图书馆与素质教育的认知解读

论述了高校图书馆在大学生素质教育中的优势,探讨了高校图书馆在实施素质教育过程中应注意的几个问题,指出素质教育给高校图书馆的建设与发展提出了新的研究课题和新的挑战。     

单节锂离子电池保护电路的改进

提出了一种低成本的单节锂电池保护回路系统,采用0.6μm混合信号CMOS工艺和修调技术使芯片具有低功耗、高精度检测电压等特点.通过基准电路和取样电路设计的改进,使保护电路实现了多种保护功能,并且具有很高的检测电压精度.模拟结果表明,该电路在温度为25℃时过充电保护电压的检测精度达到了±25mV,耗电流仅为3.5μA,满足高精度检测电压的要求.     

加快校园文化建设促进构建和谐校园

构建社会主义和谐校园是崭新事业,而校园文化建设作为和谐校园建设的重要组成部分,正越来越发挥着极为重要的促进作用,但现阶段校园文化建设还存在着一些问题,如何正确认识和把握这些问题,是推动和促进校园文化建设,进而带动和谐校园建设的首要条件。     

论城市居住区“以人为本“的绿化理念

“以人为本”是城市人居环境绿化工作的重要理念。应摒弃绿化中的急功近利思想,切实从居民的日常需求出发,努力把“以人为本”的理念贯彻于城市居住区的各类功能绿化中,并通过生态绿化使人本观念得以更深刻地阐释。     

功能性反应函数为x~(1/2)的食饵-捕食者系统的定性分析

研究功能性反应函数为x的食饵-捕食者系统dx/dt=x(a-bx1/2-x)-cyx1/2,dy/dt=y(-d ecx1/2),讨论了该系统的平衡点及极限环的存在性与唯一性及不存在性.     

浅谈英语暗喻的使用及其功能

作为一种修辞,暗喻在英语中的使用是极其普遍的,与其他修辞手段相比,暗喻有其自己的特点和使用方式。本文简要地介绍了英语暗喻的几种不同使用形式极其使用功能。