兴安柴胡化学成份研究Ⅲ

本文报道在兴安柴胡中分得10种化合物,经光谱分析和化学方法鉴定为柴胡皂甙 a(Ⅰ)、柴胡皂甙 b_2(Ⅱ),α一菠甾醇-3-0-β-D-葡萄糖甙(Ⅲ)单棕榈酸甘油酯(Ⅳ)、单亚油酸甘油酯(Ⅴ)、二亚油酸甘油酯(1,3)(Ⅵ),二亚油酸甘油酯(1,2)(Ⅶ),α-菠甾醇(Ⅶ),β-谷甾醇(Ⅸ).α-D-葡萄糖(X).     

不同频率超声对提取芸香甙成分的影响

用不同频率的超声从槐米中提取芸香甙。与热硷提取－酸沉淀法相比，此法无需加热，只需用频率为２０ｋＨｚ的超声波处理３０ｍｉｎ，提出率可提高４７．５６％，工艺简单，速度快。     

Effects of truncated mutants of the ε subunit of chloroplast ATP synthase on the fast phase of millisecond delayed light emission of chloroplast and its ATP synthesis ability

The ε subunit of the chloroplast ATP synthase and the truncated ε mutants which lack some amino acid residues from the N-terminus or C-terminus were overexpressed in E. coil When the ε subunit or the truncated ε proteins was added to the spinach chloroplast suspension, both the intensity of the fast phase of millisecond delayed light emission (ms-DLE) and the cyclic and noncyclic photophosphorylation activity of chloroplast were enhanced. With an increase in the number of residues deleted from the N-terminus, the enhancement effect of the N-terminal truncated proteins decreased gradually. For the C-terminal truncated proteins, the enhancement effect increased gradually with an increase in the number of residues deleted from the C-terminus. Besides, the ATP synthesis activity of ε-deficient membrane reconstituted with the ε subunit or the truncated ε proteins was compared. The ATP synthesis activity of reconstituted membrane with the N-terminal truncated proteins decreased gradually as the number of residues deleted from the N-terminus increased. For the C-terminal truncated proteins, the ATP synthesis activity of reconstituted membrane increased gradually with an increase in the number of residues deleted from the C-terminus, but was still lower than that of the wild type ε protein. These results suggested that: (a) the N-terminal domain of the ε subunit of the chloroplast ATP synthase could affect the ATP synthesis activity of ATP synthase by regulating the efficiency of blocking proton leakage of ε subunit; and (b) the C-terminal domain of the ε subunit of the chloroplast ATP synthase had a subtle function in modulating the ATP synthesis ability of ATP synthase.     

不同产地牡丹皮中丹皮酚和芍药甙含量的HPLC法测定

采用RP-HPLC方法建立了测定牡丹皮中丹皮酚和芍药甙含量的方法.色谱条件:C18色谱柱;丹皮酚测定所用流动相为甲醇—水(50∶50)为流动相,检测波长为274 nm,;芍药甙测定所用流动相为乙腈-0.1%磷酸溶液(18∶82)为流动相,检测波长为230 nm.结果:丹皮酚和芍药甙的线性范围分别为0.36-2.2μg/L和0.042-1.060μg/L,平均回收率分别为96.30%和98.21%,RSD分别为0.97%和0.64%.本方法简便、快捷、重现性好.用本方法对不同产地的牡丹皮中丹皮酚和芍药甙进行了测定,结果表明,牡丹皮中两种成分明显正相关,其中四川牡丹皮中两种成分含量最高.     

红景天甙对人红细胞膜流动性的影响

利用荧光技术，研究红景天天甙对人红细胞膜流动性的影响。结果表明，红景天甙对膜脂疏水链区流动性无显著影响；但红景天甙浓度增加对脂膜极性基区流动性有增强作用；红景天甙对膜蛋白也有作用。     

金沙江干热河谷蕃麻皂素资源的开发研究

借助已有的酸水解／醇提取的方法，分析了来自四川攀枝花、成都、海南西部、南非中部4个不同生境地域的共10个蕃麻鲜叶片样品，以及来自云南楚雄元谋、保山施甸、丽江永胜、四川攀枝花4个典型的金沙江干热河谷地域的15个蕃麻麻膏（干料，含水量15～20％）样品，得到了其甾体总甙元组分、含量等基础数据资料。并在此基础上，重点开展了对蕃麻皂素的提取、分离、精制工作，建立了合适的工艺技术路线，乙酰蕃麻皂素产率4．7％。结果表明：金沙江干热河谷地域很适合蕃麻生长，而且生源积累优势组分蕃麻皂甙元，利于后加工，很有希望成为我国甾体药业新的原料基地。蕃麻作为一种种植业发展潜力巨大。     

高效薄层双波长扫描法测定葛根芩连胶囊中？…

本采用硅胶Ｇ高效薄层色谱双波长扫描法对葛根芩连胶囊中葛根素和大豆甙元的含量进行了同时测定。此法快速，简便，稳定性和重现性好，板间误差小，方法可靠。葛根素平均回收率为９９．８４％，变异系数为１．３２％；大豆甙元平均回收率为９９．７６％，变异系数为１．２７％。     

小蔬菜大学问常吃7种菜防癌又长寿

茄子:"霜打茄子"是好药中药许多方剂及民间验方中,时常使用"秋后老茄子"、"霜打茄子"。越来越多证据表明,茄子具有抗癌功能。研究人员从茄子中提取的一种无毒物质,用于治疗胃癌、子宫颈癌等收到良效。另外,茄子中含有龙葵碱、葫芦素、水苏碱、胆碱、紫苏甙、茄色甙等多种生物碱物质其中龙葵碱葫芦素被证实     

葡萄糖香草醛的相转移催化合成法

在葡萄糖香草醛结构－活性相关性研究中，进行了葡萄糖香草醛的合成工作。以溴代四乙酰葡萄糖和香草醛为原料，溴化四丁基铵为催化剂，采用相转移催化法进行糖甙化反应以９２％的收率得到四乙酰葡萄糖香草醛，经脱保护基后得到葡萄糖香草醛。产物结构经元素分析、红外光谱、核磁共振谱所证实。     

杜仲翅果主要有效成分提取工艺的研究

研究杜仲翅果所含主要有效成分———杜仲油、杜仲胶和桃叶珊瑚甙的提取工艺,试验结果表明,杜仲油提取最佳工艺条件为:原料水分8.86%、提取时间15min×8次,提取率可达12.59%;杜仲胶最佳提取工艺为:浸提温度65℃、提取时间20min×5次,提取率可达8.94%;桃叶珊瑚甙采用乙醇/乙醚法提取工艺,提取率可达4.03%。