全文获取类型
收费全文 | 3071篇 |
免费 | 191篇 |
国内免费 | 39篇 |
专业分类
系统科学 | 196篇 |
丛书文集 | 127篇 |
教育与普及 | 10篇 |
理论与方法论 | 19篇 |
现状及发展 | 58篇 |
综合类 | 2891篇 |
出版年
2023年 | 1篇 |
2022年 | 5篇 |
2021年 | 4篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 13篇 |
2014年 | 705篇 |
2013年 | 489篇 |
2012年 | 393篇 |
2011年 | 402篇 |
2010年 | 424篇 |
2009年 | 419篇 |
2008年 | 376篇 |
2007年 | 13篇 |
2006年 | 7篇 |
2005年 | 1篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2002年 | 5篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
排序方式: 共有3301条查询结果,搜索用时 250 毫秒
121.
鱼类应激时主要启动两种生理反应系统,即"交感-嗜铬组织"系统/和/或"下丘脑-垂体-肾间组织轴"(HPI轴)系统.在实际生产操作中,鱼类容易受到浅水暴露应激.为了探讨黄斑蓝子鱼在受到浅水应激后HPI轴是否被激活,本研究克隆了下丘脑中启动HPI轴活性的关键因子—促肾上腺皮质激素释放激素(CRH)的基因,并检测其在应激前和受到浅水应激后不同时间点(0、20、40、60、80 min)的mRNA水平.结果显示,CRH前体的cDNA全长为1025 bp,编码170个氨基酸(aa),分别由一个信号肽(24个aa)、一个功能未知保守区(13个aa)和一个成熟肽(41个aa)组成;该CRH前体和成熟肽与其他鱼类的相应肽分别具有62%-98%和78%-100%的序列相似性.当鱼体受到短暂(4 min)浅水应激后80 min内,脑中CRH mRNA水平在应激前后无显著变化(P>0.05),说明黄斑蓝子鱼在受到急性浅水应激后可能主要启动"交感-嗜铬组织"系统而不是HPI轴. 相似文献
122.
Density functional theory (DFT) was utilized to simulate the reactions occurred in the mixture solution of cysteine and Hg(II) ions with the ratio 〈 2. Simulation result shows that Hg ions will coordinate to cysteine by the thiol groups with the form of S-Hg2 and S-Hg3; and moreover, the content of free Hg ions can only be reduced by these two forms. OH- plays an important role in the growth of β-HgS, because its nucleophilic substitution reaction supplies plenty of Hg-S-Hg radicals, which will be adsorbed onto the surface of liquid mercury ball and form the precursor of 13-HgS(111) plane. Three valent bonds adsorption of Hg-S-Hg radicals onto the surface of Hg ball has more adsorption energy (-32.768 kcal mol-1) than that of two valent bond adsorption (-20.882 kcal - mol-1). Hg balls will stop growing after completely covered with Hg-S-Hg radicals and their size will be limited. The growth direction of β-HgS is parallel to the "repel- ling" force, that is [111] direction in β-HgS lattice. The calculated results are in good agreement with the experimental observations, demonstrating that the DFT method can be taken as a very useful tool to interpret the solution reactions that cannot be solved by conventional methods. 相似文献
123.
124.
本文研究函数空间L~(p1,p2,…,pn)(X1×X2×…×Xn),它是L~P(X)的推广。同时还推广了积分型Minkowski不等式和Holder不等式。 相似文献
125.
PWB(Printed Wre Broad)是电子产品不可或缺的重要组件,PWB感光制作具有制作方便、出板快、成本低等优点.本文基于近百次PV4B感光制作,研究分析了感光制板各阶段操作要领,提出了确保PWB制作精度和成功率的改进措施.实践表明:本文强调的操作要领是决定制作成功与否的关键,提出的改进措施可以提高制板精度,最小线宽、间距可达4.7mil,接近PCB专业厂家的工艺能力. 相似文献
126.
Metabonomic analysis of hepatitis B virus-induced liver failure: identification of potential diagnostic biomarkers by fuzzy support vector machine 下载免费PDF全文
Yong MAO ;Xin HUANG ;Ke YU ;Hai-bin QU ;Chang-xiao LIU ;Yi-yu CHENG 《浙江大学学报(自然科学英文版)》2008,(6):474-481
Hepatitis B virus (HBV)-induced liver failure is an emergent liver disease leading to high mortality. The severity of liver failure may be reflected by the profile of some metabolites. This study assessed the potential of using metabolites as biomarkers for liver failure by identifying metabolites with good discriminative performance for its phenotype. The serum samples from 24 HBV-indueed liver failure patients and 23 healthy volunteers were collected and analyzed by gas chromatography-mass spectrometry (GC-MS) to generate metabolite profiles. The 24 patients were further grouped into two classes according to the severity of liver failure. Twenty-five eommensal peaks in all metabolite profiles were extracted, and the relative area values of these peaks were used as features for each sample. Three algorithms, F-test, k-nearest neighbor (KNN) and fuzzy support vector machine (FSVM) combined with exhaustive search (ES), were employed to identify a subset of metabolites (biomarkers) that best predict liver failure. Based on the achieved experimental dataset, 93.62% predictive accuracy by 6 features was selected with FSVM-ES and three key metabolites, glyeerie acid, cis-aeonitie acid and citric acid, are identified as potential diagnostic biomarkers. 相似文献
127.
Determination of risperidone in human plasma by HPLC-MS/MS and its application to a pharmacokinetic study in Chinese volunteers 下载免费PDF全文
Ming-zhu HUANG Jian-zhong SHENTU Jun-chun CHEN Jian LIU Hui-li ZHOU 《浙江大学学报(自然科学英文版)》2008,(2):114-120
This study presents a rapid, specific and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of risperidone (RIS) in human serum using paroxetine as an internal standard (IS). An Alltima-C18 column (2.1 mm×100 mm, 3 μm) and a mobile phase consisting of 0.1% formic acid-acetonitrile (40:60, v/v) were used for separation. The analysis was performed by selected reaction monitoring (SRM) method, and the peak area of the m/z 411.3→191.1 transition for RIS was measured versus that of the m/z 330.1→192.1 transition for IS to generate the standard curves. The assay linearity of RIS was confirmed over the range 0.25-50.00 ng/ml and the limit of quantitation was 0.05 ng/ml. The linear range corresponds well with the serum concentrations of the analytes obtained in clinical pharmacokinetic studies. Intraday and interday relative standard deviations were 1.85%-9.09% and 1.56%-4.38%, respectively. The recovery of RIS from serum was in the range of 70.20%-84.50%. The method was successfully applied to investigate the bioequivalence between two kinds of tablets (test versus reference products) in 18 healthy male Chinese volunteers. The result suggests that two formulations are bioequivalent. 相似文献
128.
“靓”本是一个古语词和方言词,曾一度消失于汉语普通话中。随着粤语的北上,“靓”由粤方言进入共同语中泛化使用。近十余年来,“靓”的使用在全国尤其是在粤港地区人们日常生活口语中呈现一种滥用的状态,其词义的感情色彩在逐渐发生转移。 相似文献
129.
Jianhua Liu Yong Wei 《系统科学与信息学报》2008,6(1):91-96
This article gives the algorithm of the sector in which the development coefficient is, and the steps to simultaneously calculate the development coefficient and combination coefficient of background value. Unifying the development coefficient and the background value's coefficient of combination to optimize the model together, avoiding the malpractice to separate the two in traditional method, and avoiding the error brought out by matrix multiplication because of matrix's illness and so on in the traditional way, therefore increases the precision of the model. 相似文献
130.