拟南芥中与AtGluRS相互作用蛋白质的初步研究

AtGluRS是ABA信号通路的正调节因子,利用酵母双杂交从拟南芥cDNA表达文库中筛选出一个与AtGluRS相互作用的未知蛋白(UGT).为了研究蛋白功能,构建真核过表达和抑制表达载体,用农杆菌介导花序侵染法转化野生型拟南芥,筛选鉴定转基因植株并进行初步的生理性状分析.     

Involvement of NADPH oxidase NtrbohD in the rapid production of H2O2 induced by ABA in cultured tobacco cell line BY-2

The mechanisms for the production of hydrogen peroxide (H₂O₂) induced by abscisic acid (ABA) were investigated in suspension culture cells of tobacco BY-2 cells. The results showed that the immediate generation of H₂O₂, which was mainly derived from superoxide dismutase-catalyzed dismutation of superoxide radical, was significantly induced by ABA. Furthermore, treatment of the cultured tobacco cells with ABA resulted in a time-dependent quick increase in plasma membrane (PM) NADPH oxidase activity, which coincided on time and magnitude with the elevation in ABA-induced accumulation of H₂O₂. Moreover, these enhanced effects were pronouncedly inhibited by two NADPH oxidase inhibitor, diphenylene iodonium and imidazole, suggesting that PM NADPH oxidase is involved in the rapid accumulation of ₂O₂ in cultured tobacco cells. In addition, analysis of the expression level of NtrbohD, a PM NADPH oxidase gene in tobacco, by RT-PCR and protein gel blot revealed that the gene at both mRNA and protein levels was upregulated by ABA, indicating that NtrbohD participates in the ABA-stimulated rapid production of H₂O₂ in tobacco culture cells. Taken together, these findings suggest that ABA induces the rapid accumulation of reactive oxygen species via NADPH oxidase in suspension culture cells of tobacco, and that NADPH oxidase and H₂O₂ appear to be important components in ABA signal transduction pathway in plants.     

不同外源激素处理对长白松种子发芽能力的影响

选用不同浓度吲哚丁酸、萘乙酸及赤霉素浸种1,2 h,对长白松种子进行预处理,以清水浸种2 h为对照,试验分析预处理后长白松种子的萌发状况.结果表明:长白松种子经不同外源激素处理后发芽率、发芽势明显提高.其中,以100 mg/L的IBA浸种2 h长白松种子的发芽效果最好,发芽率为53.66%,发芽势为12.33%,发芽指数为38.15.     

IAA,IBA,NAA和2,4—D对槐树试管苗生根的影响

ＩＡＡ和ＩＢＡ诱导槐树试管苗形成的不定根细而长，直接来源于茎，根系发达．ＮＡＡ诱导试管苗基部先形成愈伤组织，然后由愈伤组织上产生不定根，其根短而粗．ＮＡＡ诱导的生根率最高，但其根生长缓慢，表面常愈伤组织化．２，４－Ｄ促进苗基部产生大量的愈伤组织，对生根有抑制作用     

基于图像型的煤矿早期外因火灾预测及识别方法研究

针对传统煤矿井下火灾预测易受环境影响的不足,文中提出了一种基于图像型的煤矿外因火灾预报方法。文中基于火灾图像特征根据运动检测提取出疑似火灾区域,再根据颜色决策排除掉不具有火焰颜色的物体,最后再利用离散分形布朗增量场进行识别。实验结果表明,该算法对于小样本、非线性的分类问题效果显著,该法能较好地将煤矿井下的火源与干扰源区别开来,有较高的识别率和较强的鲁棒性,可降低误报、漏报。     

NCED3基因雌二醇诱导表达对ABA合成酶基因和代谢酶基因表达的影响

胞内ABA信号系统是否存在正反馈调节,一直存在争论.借助于雌二醇诱导的基因表达体系,对ABA合成关键酶基因NCED3超表达,结果发现,由于NCED3限速酶基因的高表达提高了内源ABA信号水平,进而上调了ABA合成酶基因ZEP、AA03等的表达水平,-gitt~了代谢酶基因CYPT07A3的表达.研究显示内源ABA信号水平存在正负两种反馈调节机制,植物特定生理过程中ABA信号池的高低是两种调节的协同结果.     

Involvement of carbon monoxide produced by heme oxygenase in ABA-induced stomatal closure in Vicia faba and its proposed signal transduction pathway

Carbon monoxide (CO) has recently proven to be an important bioactive or signaling molecule in mammalian cells. Its effects are mainly mediated by nitric oxide (NO) and cyclic GMP (cGMP). In Vicia faba leaves, CO production and heme oxygenase (HO) activity, an important CO synthetic enzyme, are first reported to increase in response to ABA treatment, which could result in stomatal closure. Inter- estingly, ABA-induced stomatal closure in V. faba guard cells is partially blocked when the synthetic CO inhibitor ZnPP, or the CO/NO scavenger Hb is added. Furthermore, we show that, exogenously applied CO donor, hematin, and CO aqueous solution not only result in the enhancement of CO release, but also time-dependently induce stomatal closure, and the latter is mimicked by the application of an NO donor SNP. The above-mentioned stomatal closure effects are differentially reversed by the addition of tungstate, a potent inhibitor of NO synthetic enzyme nitrate reductase (NR), the specific NO scavenger cPTIO, ZnPP, or Hb. During treatment for 4 h, SNP, 0.01% CO aqueous solution or hematin significantly triggers NO synthesis, whereas cPTIO, or tungstate approximately fully inhibits NO fluorescence. Ad- ditionally, application of the GC inhibitor ODQ blocks CO-induced stomatal closure. This inhibition could be reversed when 8-Br-cGMP is added. Thus, the above results suggest that CO produced by HO is involved in ABA-induced stomatal closure, and NO and cGMP may function as downstream interme- diates in the CO signaling responsible for stomatal closure.     

植物生长物质与植物雄性不育研究进展

对植物生长物质与植物雄性不育关系的研究现状进行了综述,已有的研究涉及到雄性不育与各种内源激素水平的关系、外施激素对植物育性的影响、以及植物生长物质在雄性不育中的作用机理等,不同生长物质对育性表达作用不同：GAs和多胺有利于雄性器官的发育,生长素、细胞分裂素、乙烯和脱落酸含量过高往往导致雄性不育,雄性不育既受单种生长物质变化的影响。也受各种生长物质间平衡的影响。     

ABA和H2 O2在NaCl诱导的气孔关闭中的作用

以拟南芥野生型（wt）和ABA合成缺失突变体（aba3）为材料，采用表皮生物分析法研究了NaCl胁迫条件下，ABA和H2O2在气孔关闭中的作用。结果表明：100mmol/L的NaCl可有效诱导wt气孔关闭，而对aba3的气孔运动无明显影响；10^-2mmol/L的H2O2处理或100mmol/L的NaCl与10^-2mmol/L ABA共处理可有效诱导wt与aba3的气孔关闭，且幅度类似；H2O2的性清除剂CAT可部分逆转NaCl处理及NaCl与ABA共处理引起的气孔关闭，因此推测，NaCl胁迫条件下，植物保卫细胞内ABA浓度升高，诱导H2O2的产生，进而诱导了气孔关闭。