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1.
为了深入研究Kirchhoff方程的性质,讨论了带有Hartree项和临界增长非线性项的Kirchhoff方程极小能量变号解的存在性。利用能量泛函在变号Nehari流形上的下确界C_λ收敛于0,得到空间E紧嵌入L~6(R~3)这一技术性结果。结果表明,利用限制变分方法和定量形变引理获得极小化序列对应的极小值点是该问题的非平凡解。研究方法在理论证明方面得到了良好的结果,对研究其他Kirchhoff方程解的存在性有一定的指导意义。 相似文献
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《科学通报(英文版)》2008,(19)
The purposes of this research were to study the stable expression of exogenous gene encoding therapeutic protein in attenuated Salmonella typhimurium, observe the metabolism of oral gene vaccine carried by attenuated Salmonella typhimurium in BALB/c mouse, and investigate the feasibility of prevention and treatment of tumors by the recombinant bacteria. Recombinant plasmid pcDNA3.1 VEGFR2(n1-7) was transformed into competent attenuated Salmonella typhimurium SL3261 to develop oral DNA vaccine SL3261-pcDNA3.1 VEGFR2(n1-7). To observe whether the exogenous gene can be expressed in the recombinant bacteria, PCR was performed to amplify the CMV promoter of the eukaryotic expression vector as the proof of stable expression of exogenous protein; transmission elec- tron microscopy (TEM) was applied to observe the morphology of the recombinant bacteria to confirm that the exogenous gene has no impact on the growth of the bacteria, and then BALB/c mice were immunized with the gene vaccine. After inoculation of the gene vaccine, the recombinant bacteria SL3261 could be detected in the tissues such as small intestine, colon, liver and spleen. And then, mice in each group were challenged with tumor cells. The results of animal experiment showed that tumor growth of the mice in experimental group was inhibited and survival time of immunized mice was prolonged compared with control groups. A higher lymphocyte infiltration in tumors from animals treated with DNA vaccine was observed. Immunohistochemical analysis of tumor samples revealed an en- hanced accumulation of CD8 cytotoxic T lymphocytes, as well as an increase in CD4 cells in the tumors of animals treated with the oral gene vaccine compared to tumors from control group mice. Ultrastructure of the tumor tissue showed that tumor cells in the samples of the immunized mice were well-differentiated. Our research confirmed that the exogenous gene can be stably expressed in the attenuated Salmonella typhimurium and has no impact on the growth of the recombinant bacteria; the exogenous gene can de delivered to the host by attenuated Salmonella typhimurium to produce anti-tumor effect with no obvious cytotoxity to the host. In this study, it is established that attenuated Salmonella typhimurium could be used as a vector for oral gene vaccine, and our study provided a theoretical basis for the body distribution and the metabolism of the recombinant bacteria. This strategy may provide a simple, safe and effective way for the prevention and treatment of tumors. 相似文献
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试验表明,“926”植物生长调节剂对植物种子活力、根系活力、植物生长发育具有明显的促进作用。用“926”浸稻种和玉米种,可提高发芽势、发芽率,增加次生根数和次生根长。苗期施药,同样可促进幼苗生长发育、促进根系发育、增加分蘖、分枝,提高成穗率。其它生育期施药,可分别提高成稳率、穗粒数、粒重,显著提高作物产量。 相似文献
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上皮生长因子预防心脏术后心包粘连的实验及临床研究 总被引:1,自引:0,他引:1
目的探讨上皮生长因子(EGF)对心脏术后心包粘连的预防作用.方法术中对犬心包内喷洒上皮生长因子3个月后,观察心包粘连情况;选先天性心脏病、房缺及室缺病人40例,术中心包内喷洒上皮生长因子,术后6个月、12个月经CT检查,判定心包粘连、增厚结果.结果在动物实验和临床研究中,实验组粘连程度显著低于对照组(P<0.01).结论 EGF心包内应用有明显预防心脏术后心包粘连的作用. 相似文献
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Droplets of Al-32.7%Cu eutectic alloy were rapidly solidified during containerless processing in a 3 m drop tube. The microstructural evolution of Al-Cu eutectic alloy depends strongly on droplet size. The eutectic growth morphology changes from regular lamellar eutectic to a kind of anomalous eutectic with the decrease in droplet size. This microstructural transition was analyzed within the current theories of eutectic growth. The results indicate that there exists a critical velocity for eutectic growth beyond which anomalous eutectic appears. The coupled zone of the Al-Cu eutectic alloy system has been calculated on the basis of the Trivedi-Magnin-Kurz(TMK) eutectic growth and the Lipton-Kurz-Trivedi(LKT)/Boettinger-Coriell-Trivedi(BCT) dendrite growth models, which provides a further interpretation for the morphological transition. 相似文献
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在形状记忆合金中,存在马氏体穿晶生长现象,它是热弹马氏体相变在小角晶界的行为。 相似文献
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本文讨论了生长曲线模型中多组数据对回归分析的联合影响问题,给出了度量准则及其化简式与统计解释,推广了已有文献中的有关结果. 相似文献