Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.     

AC2 and AC4 proteins of <Emphasis Type="Italic">Tomato yellow leaf curl China virus</Emphasis> and <Emphasis Type="Italic">Tobacco curly shoot virus</Emphasis> mediate suppression of RNA silencing

Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) alone could systemically infect host plants such as Nicotiana benthamiana without symptoms. In contrast, Tobacco curly shoot virus Y35 isolate (TbCSV-Y35) alone induces leaf curl symptoms in N. benthamiana. When inoculated into transgenic N. benthamiana plants expressing GFP gene (line 16c), TYLCCNV-Y10 neither reverses the established GFP silencing nor blocks the onset of GFP silencing. In contrast, TbCSV-Y35 can partially reverse the established GFP silencing and block the onset of GFP silencing in new leaves. In the patch co-infiltration assays, the AC2 and AC4 proteins of TYLCCNV-Y10 and TbCSV-Y35 could suppress local GFP silencing and delay systemic GFP silencing, suggesting that they are suppressors of RNA silencing. Comparison of the accumulation levels of GFP mRNA in the co-infiltration patches showed that Y10 AC2 and Y35 AC2 proteins had similar efficiency for suppression of RNA silencing. However, Y35 AC4 protein functioned as a stronger suppressor of RNA silencing than Y10 AC4 protein. There-fore, the pathogenicity difference between TbCSV-Y35 and TYLCCNV-Y10 may be related to the functional difference in their AC4 proteins.     

Enzymes and receptors in the leukotriene cascade

Leukotrienes are a family of paracrine hormones derived from the oxidative metabolism of arachidonic acid. These lipid mediators are recognized as important signal molecules in a variety of inflammatory and allergic conditions affecting the skin, joints, gastrointestinal and respiratory systems, in particular asthma. Such conditions are typified by local pain, tissue edema, hyperemia and functional losses. In the tissues, immunocompetent cells accumulate at the site of injury which contribute to tissue damage and perpetuation of the disease process. Leukotrienes can elicit most, if not all, of these signs and symptoms. Thus, leukotriene B₄ is one of the most powerful chemotactic agents known to date and participates in the recruitment of leukocytes. The cysteinyl leukotrienes, on the other hand, contract smooth muscles, particularly in the peripheral airways and microcirculation. Recently, drugs which block the formation and action of leukotrienes have been introduced as novel antiasthmatic medications. This chapter reviews the biochemistry, molecular biology and cell biology of the key enzymes and cognate receptors in the leukotriene cascade.     

对新型计算机病毒的认识

对传统型计算机病毒与新型计算机病毒的特点、传播途径等进行了总结和比较,重点介绍了3种最新的计算机病毒的特征及其危害性,它们分别是“苏珊”病毒、“笑脸”病毒和“9·11”计算机病毒。     

印度记数法在伊斯兰世界的传播

主要论述印度记数法是在怎样的背景下传入伊斯兰世界,在伊斯兰世界的传播都经历了哪些曲折的过程,最终又是怎样流入西欧的,中世纪穆斯林学为印度记数法的传播做出了重要贡献,中重点介绍印度记数法最早的宣传花拉子米所写的第一本专门介绍印度记数法的作《印度算术书》,并进一步探讨了这部作是根据哪些来源于印度的材料完成的。     

Adsorption and catalytic combustion of ARB on CuO-Fe<Subscript>2</Subscript>O<Subscript>3</Subscript>

CuO-Fe2O3 composite material with strong magnetism and a large surface area is prepared by the co-precipitate method. Its adsorption properties towards Acid Red B (ARB) and the regeneration by catalytic combastion of organic compounds have been studied. The results show that the prepared CuO-Fe2O3 composite is an excellent adsorbent for ARB adsorption at acid condition. The presence of Cl^- has no effect on ARB adsorption. But the SO4^2- can inhibit ARB adsorption. After being recovered by the magnetic separation method, the adsorbent can be regenerated by catalytic oxidation of absorbate at 300℃ in air atmosphere. The combustion reactions of ARB in the presence or absence of CuO-Fe2O3 are studied by in situ diffuse refieclion FTIR. The results indicate that, in the presence of CuO-Fe2O3, the degradation temperature is significantly lowered by the catalysis of CuO-Fe2O3, and ARB can be oxidized completely without volatile organic compound by-product; in comparison, in the absence of CuO-Fe2O3, the temperature needed for oxidation of ARB is higher and the reaction is incomplete with some N-containing harmful compounds produced. The reusability of CuO-Fe2O3 is also studied in successive seven adsorption-regeneration cycles.     

综合航电火控系统模拟系统设计

当前军机广泛的使用指令/响应型数据总线作为其多功能综合航空电子火控系统的数据传输和控制系统,应用实时数据库的数据管理方法,采用面向组件的方法,仿真1553B总线的总线控制器和各远程终端的运行,设计实现了这种类型的专用综合航电模拟系统,并满足了实时性的要求。     

浅析CSL160B系列保护装置中重合闸启动方式

以CSL160B系列数字式保护装置中重合闸启动方式为例,分析了此种启动方式和常规的重合闸启动方式的不同,探讨了存在的问题,并提出了解决办法。     

浅谈校园网络的安全

介绍了搞好校园网络安全的主要措施,包括加强网络监控、注意密码的安全、及时修补漏洞、设置共享时一定要设定访问密码等。     

预退火对Fe73.5Cu1Nb3Si13.5B9纳米晶合金巨磁阻抗效应的影响

用HP4294A型阻抗分析仪测量了经不同温度预退火后再540℃退火的Fe73.5Cu1Nb3S i13.5B9纳米晶合金薄带的巨磁阻抗,并结合XRD衍射图谱和AFM图谱,研究了预退火对纳米晶介观结构的影响.结果发现,200℃、300℃和400℃预退火处理40 m in对随后540℃退火的Fe73.5Cu1Nb3S i13.5B9薄带-αFe(S i)纳米晶介观结构产生了影响,颗粒团聚优势明显减弱,横向各向异性场减小,巨磁阻抗比得到了显著的提高.